| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,556,769 | (T)9→10 | intergenic (‑449/‑224) | yjiC ← / → iraD | uncharacterized protein/RpoS stabilzer after DNA damage, anti‑RssB factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,556,760 | 1 | . | T | 98.0% | 189.6 / ‑2.9 | 50 | intergenic (‑440/‑233) | yjiC/iraD | uncharacterized protein/RpoS stabilzer after DNA damage, anti‑RssB factor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (25/24); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
ATCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGG > NC_000913/4556612‑4556905 | aTCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑t < 1:758139/150‑1 (MQ=255) ttATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑ttttttt < 2:338346/150‑1 (MQ=255) ttATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑ttttttt < 2:376397/150‑1 (MQ=255) atatTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑tttttttttt < 2:704908/151‑2 (MQ=255) attTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑tttttttttttgagt < 1:177573/151‑7 (MQ=255) ttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGttt < 1:525851/150‑1 (MQ=255) ttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTtg > 1:449610/1‑151 (MQ=255) aaCTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTgatgat > 1:325279/1‑151 (MQ=255) gTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCa < 1:757777/151‑1 (MQ=255) tCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTTCCACCGTAATTAAGTAGCAGCGGTGACAAGTATTTTAATCTACGTAACTATTCGGAATATTTAGAATATACTAGCGGT‑TTTTTTTTTTAGTTTGATGATTTTTTTCaaa > 2:700767/1‑150 (MQ=255) aataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATTTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGa < 2:194456/150‑1 (MQ=255) aataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGa < 2:539728/150‑1 (MQ=255) ataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACt > 2:375693/1‑151 (MQ=255) taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtt > 2:404379/1‑151 (MQ=255) tGGGTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtttttt > 1:84790/1‑151 (MQ=255) tGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtttttt > 2:184573/1‑151 (MQ=255) gCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTcc < 1:18844/151‑1 (MQ=255) gATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCt < 2:838070/149‑1 (MQ=255) gATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTg > 2:840473/1‑150 (MQ=255) tATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaaa < 2:884955/150‑1 (MQ=255) gCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGt > 1:475244/1‑150 (MQ=255) cGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc > 2:298199/1‑150 (MQ=255) ggAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc > 1:218768/1‑149 (MQ=255) gAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGt < 1:401589/151‑1 (MQ=255) aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat < 1:185693/151‑1 (MQ=255) aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat < 1:886423/150‑1 (MQ=255) aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGatat < 2:900184/151‑1 (MQ=255) aaataaTTTCCCACCGGAATTAAGTAGCAGCCGTGGAAAGTATTTTCATCTACGTAACTAATCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGata < 2:155370/151‑1 (MQ=255) cACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCaa < 2:16246/151‑1 (MQ=255) cACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCaa < 1:473208/151‑1 (MQ=255) cGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGt > 1:494253/1‑150 (MQ=255) gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAg < 1:22559/151‑1 (MQ=255) aaGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGGGTTTGATGATTTTTATAAAATAATAAATTTTTTCCTGAAAAGTAGGTAGTTATTCAAAGGAAAGTAAGGttt > 1:417917/1‑150 (MQ=255) gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat < 2:449060/151‑1 (MQ=255) agcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACttt < 1:758278/151‑1 (MQ=255) aTTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTTCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGAtt > 2:314283/1‑150 (MQ=255) aTTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTc < 1:720574/151‑1 (MQ=255) ttttTATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTTTGTGTTTTATGGTTTTTTTCAAATCATGCCTTTTTTCCTTAAAAGTCAGTAGATATTCATAGGCAAGAAAGGCTTTATACTTTTCTGACAGGAtt > 2:545623/1‑150 (MQ=255) ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAg < 1:559152/150‑1 (MQ=255) tCTACGTAACTATTCAGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTg > 2:385852/1‑151 (MQ=255) cTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGt > 2:882792/1‑151 (MQ=255) aCGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct > 1:461764/1‑151 (MQ=255) aCGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtc > 1:270363/1‑150 (MQ=255) aCGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGt < 2:494204/149‑1 (MQ=255) cGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtctc < 1:679738/151‑1 (MQ=255) gTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa < 2:690021/151‑1 (MQ=255) tAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATATGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa < 2:482860/150‑1 (MQ=255) tAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGCTTGATGATTTTTATCAAATAATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATCGGCAAGTAAGGGTTTATACTTTGCTGACAGGATTCGGGCATGtacc > 1:218996/1‑146 (MQ=255) atTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGt < 1:370438/151‑1 (MQ=255) cAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAAt > 1:823527/1‑151 (MQ=255) aaTATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGa > 1:218285/1‑150 (MQ=255) atatACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAAc < 2:420068/151‑1 (MQ=255) taCTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACaa > 2:775075/1‑150 (MQ=255) tAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTTAAAAGTCATTCGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACACAAgg > 2:741187/1‑151 (MQ=255) aGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAggg > 1:59980/1‑151 (MQ=255) | ATCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGG > NC_000913/4556612‑4556905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |