Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	597,758	(G)5→6	coding (280/1224 nt)	cusB →	copper/silver efflux system, membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	597,753	1	.	G	100.0%	197.7 / NA	54	L92R (CTG→CGG)	cusB	copper/silver efflux system, membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/30);  total (24/30)

CTTATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCT‑GGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGGTGTATCCGCTT  >  NC_000913/597604‑597892                                                                                                                                                       |                                                                                                                                            cTTATTCTGGTAAGACACAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGCGCGCCTTGACCCGACTCAGACGCAGAATCT‑g                                                                                                                                            >  2:384015/1‑151 (MQ=255)  ttATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCT‑gg                                                                                                                                           >  2:250235/1‑151 (MQ=255)       cTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCT‑gggggg                                                                                                                                       >  2:586577/1‑149 (MQ=255)             cGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAAc                                                                                                                                 <  2:789163/151‑1 (MQ=255)             cGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAAc                                                                                                                                 <  1:621019/151‑1 (MQ=255)               aCCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGGGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACgg                                                                                                                               >  2:572417/1‑151 (MQ=255)                  cAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCt                                                                                                                             >  1:40459/1‑150 (MQ=255)                  cAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTa                                                                                                                            <  2:174574/151‑1 (MQ=255)                   aaTGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTAc                                                                                                                           <  2:830736/151‑1 (MQ=255)                      gTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCg                                                                                                                         <  1:392726/150‑1 (MQ=255)                      gTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCg                                                                                                                         <  2:167406/150‑1 (MQ=255)                        aTCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTc                                                                                                                       >  1:310738/1‑150 (MQ=255)                            cAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCAcgcg                                                                                                                  >  2:342388/1‑151 (MQ=255)                             aaTACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCAcgcgc                                                                                                                 >  1:101681/1‑151 (MQ=255)                              aTACGCGGTGCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGGCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGCCTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCAcgcgcg                                                                                                                <  2:232508/151‑1 (MQ=255)                               tACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCgg                                                                                                               >  1:319056/1‑151 (MQ=255)                                aCGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATACGGATCTGGTGCCGAAATTTGCTGATTAAGAGAGTTCTGCGTCTGGTGTGCGCAGTGACCCGTCTCAGACGAAGAATCTGGGGGGGGAAAACGGCTACCGTCAcgcgcg                                                                                                                <  2:614744/149‑1 (MQ=255)                                  gcgGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGAcc                                                                                                            <  1:559107/151‑1 (MQ=255)                                     gTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGc                                                                                                          >  2:843236/1‑150 (MQ=255)                                     gTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCt                                                                                                         >  1:461628/1‑151 (MQ=255)                                        cGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGa                                                                                                       <  2:438733/150‑1 (MQ=255)                                         gATAAACCAGGTAAATCGCCTTTTATGGATATGGATCTGGTGCCGAAATAGGCGGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCGGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGGGCGCTGACt                                                                                                     <  2:424194/151‑1 (MQ=255)                                         gATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTGCTGCGTCTGGTGTGCGCATTGCCCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGa                                                                                                       <  2:257782/149‑1 (MQ=255)                                            aaaCCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACtttt                                                                                                  >  1:131251/1‑151 (MQ=255)                                               ccAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGcc                                                                                               <  2:157108/151‑1 (MQ=255)                                                cAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGccc                                                                                              >  2:389122/1‑151 (MQ=255)                                                    tAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCag                                                                                            >  2:765014/1‑149 (MQ=255)                                                     aaaTCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGt                                                                                         >  2:130836/1‑151 (MQ=255)                                                         cGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTcc                                                                                     >  2:556381/1‑151 (MQ=255)                                                         cGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTcc                                                                                     <  1:752680/151‑1 (MQ=255)                                                           ccGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCg                                                                                   <  2:820149/151‑1 (MQ=255)                                                                tatggatatggaTCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGa                                                                               >  2:19493/1‑150 (MQ=255)                                                                 atggatatggatCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAAt                                                                             >  2:14994/1‑151 (MQ=255)                                                                 atggatatggatCTGGCGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAAt                                                                             >  2:373839/1‑151 (MQ=255)                                                                      tatggatCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTc                                                                          >  2:678050/1‑149 (MQ=255)                                                                         ggatCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGtt                                                                      <  2:591653/150‑1 (MQ=255)                                                                               ggTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAAc                                                                 <  1:345876/149‑1 (MQ=255)                                                                                gTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACg                                                                >  1:685074/1‑149 (MQ=255)                                                                                gTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAg                                                              <  2:896312/151‑1 (MQ=255)                                                                                        atatGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTa                                                      <  2:189692/151‑1 (MQ=255)                                                                                                aTGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATtg                                              <  1:313648/151‑1 (MQ=255)                                                                                                  gAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATtgtg                                            >  2:492859/1‑151 (MQ=255)                                                                                                    agagagTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCa                                          <  1:256871/151‑1 (MQ=255)                                                                                                           tCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTcgc                                   >  1:532802/1‑151 (MQ=255)                                                                                                           tCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTcg                                    <  2:310712/150‑1 (MQ=255)                                                                                                               cGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCt                                >  1:462915/1‑150 (MQ=255)                                                                                                                  cTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCg                            >  1:691105/1‑151 (MQ=255)                                                                                                                   tGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCgg                           >  1:563139/1‑151 (MQ=255)                                                                                                                    ggTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCggg                          <  1:60587/151‑1 (MQ=255)                                                                                                                         gcgcATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTa                      <  2:647417/150‑1 (MQ=255)                                                                                                                            cATTGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCg                   <  1:861752/150‑1 (MQ=255)                                                                                                                               tGACCCGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACa                <  1:257690/150‑1 (MQ=255)                                                                                                                                  cccGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAgg             <  2:208346/150‑1 (MQ=255)                                                                                                                                  cccGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGgt            <  1:806421/151‑1 (MQ=255)                                                                                                                                  cccGACTCAGACGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGgt            <  2:255084/151‑1 (MQ=255)                                                                                                                                            aCGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGGTGTATCCGCtt  >  2:777141/1‑151 (MQ=255)                                                                                                                                            aCGCAGAATCTGGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGGTGTATCCGCtt  <  1:859781/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                            CTTATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCT‑GGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGGTGTATCCGCTT  >  NC_000913/597604‑597892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑