Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	(G)7→8	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,189	1	.	G	100.0%	145.0 / NA	42	intergenic (‑150/+218)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (23/19);  total (23/19)

TTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTG  >  NC_000913/1351043‑1351333                                                                                                                                                    |                                                                                                                                                 tttAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑gggg                                                                                                                                              >  2:230178/1‑151 (MQ=255)   tAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑gggggg                                                                                                                                            >  2:903971/1‑151 (MQ=255)       ccTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑ggggggggcg                                                                                                                                        <  2:211148/151‑4 (MQ=255)          tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑ggggggggcgct                                                                                                                                      <  1:816661/150‑6 (MQ=255)           aTTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCtt                                                                                                                                      <  2:380457/150‑1 (MQ=255)                 gATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTcccc                                                                                                                                >  1:2012/1‑150 (MQ=255)                     cTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTTCCCTCAcc                                                                                                                           >  1:648534/1‑151 (MQ=255)                       tgttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCAcc                                                                                                                           <  2:553196/149‑1 (MQ=255)                          tgtgCCTGAAAATCAGGCAATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTTCCCTCACCCTaa                                                                                                                       >  2:267476/1‑150 (MQ=255)                            tgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAccc                                                                                                                    >  1:402493/1‑151 (MQ=255)                              ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCctc                                                                                                                  >  2:804819/1‑151 (MQ=255)                                     aTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCACCCCAACCCTcccccccg                                                                                                           >  2:187465/1‑149 (MQ=255)                                       cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCagag                                                                                                         <  1:155291/151‑1 (MQ=255)                                          gCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgggg                                                                                                      <  1:881467/151‑1 (MQ=255)                                           cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc                                                                                                     <  1:10771/151‑1 (MQ=255)                                           cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc                                                                                                     <  1:725654/151‑1 (MQ=255)                                                cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg                                                                                                <  1:486015/151‑1 (MQ=255)                                                cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg                                                                                                >  2:576159/1‑151 (MQ=255)                                                cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCCCCCCAGAGGGGGGTggg                                                                                                >  1:240236/1‑151 (MQ=255)                                                cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCCCCCCAACCCCCCCCCCAGAgggggggggg                                                                                                >  1:461431/1‑151 (MQ=255)                                                 gtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCTACCTGGCTGTGAGTAGCTATAGTTGCCCGGACCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCGAACCCTAACCCCCCTCCCCGGGGGGCGAgggg                                                                                               >  1:87263/1‑151 (MQ=255)                                                   cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAc                                                                                             <  1:294191/151‑1 (MQ=255)                                                           tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGGAtt                                                                                       >  2:799125/1‑149 (MQ=255)                                                            aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                    >  2:307600/1‑151 (MQ=255)                                                            aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                    <  2:2012/151‑1 (MQ=255)                                                              aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCACCCTAAACCTCTCCCCCGAGGGGCGAGGGGACCGTATtgtg                                                                                    >  1:626950/1‑149 (MQ=255)                                                                  tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTGCCCTTACCCTAATCCCCTCCCCAGAGGGGCGAGGGGACCCTATTGTGCAAat                                                                               >  2:894882/1‑150 (MQ=255)                                                                   aCGTACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAata                                                                              <  1:583159/150‑1 (MQ=255)                                                                       aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCCCCCTAAAACTCCCCCCCGAGGGGGGGGGGGGCCCTATtgtggcaaaattttt                                                                         >  1:480830/1‑140 (MQ=255)                                                                        cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCTCCTCACCCTATCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGtt                                                                         <  1:876495/150‑1 (MQ=255)                                                                             aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcccc                                                                    <  2:264840/150‑1 (MQ=255)                                                                                cGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCTCCCCCGAGGGGCGAGGGGGACGTATTGTGCAAATATTTTTAccc                                                                     >  2:733536/1‑146 (MQ=255)                                                                                     attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                           <  1:16419/151‑1 (MQ=255)                                                                                            tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCCCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCACCAAAAAACAGGCTc                                                    >  2:585096/1‑151 (MQ=255)                                                                                                ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCCAACCCCCTCCCCAGAGGGGCGGGGGGACCGCCTTGGGCAAATATTGTTTTCCCAGCAACAAACAAGCTCATa                                                 >  1:359569/1‑150 (MQ=255)                                                                                                      tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTTCCCCCACCCCAACCCCCTCCCCCGAGGGGCGGGGGGACCGTATTTTGCAAATATTTTTACCCCAGCAAAAAAAAGGCCCATACAGcccc                                          >  1:402427/1‑151 (MQ=255)                                                                                                      tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAACCCCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTTTTACCCCAGCAACAAACAGGCTCATACAGcccc                                          >  2:204403/1‑151 (MQ=255)                                                                                                           gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                     <  1:776694/151‑1 (MQ=255)                                                                                                              gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCCAACCCTCTCCCCCGAGGGGCGAGGGGACCGGATTGTGCAAATATTGTTACCCCCCCAACAAACAAGCTCATACAGCCCCCAACCCtt                                  >  2:473318/1‑151 (MQ=255)                                                                                                                   aGTTGCCAGGTCCGACCGGAGCAGGCGGCGGGAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCag                              <  2:668435/150‑2 (MQ=255)                                                                                                                    gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                            <  1:395233/151‑1 (MQ=255)                                                                                                                        ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTTCCCTCCCCCCAACCCCCTCCCCCGAGGGGGGAGGGGGCCGTATTTTGCCAAAATTTTTACCCCAGCAAAAAAAAAGGCCATAAAACCCCCAAACCCTTCATTGCGa                         >  1:760766/1‑150 (MQ=255)                                                                                                                        ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGAt                        <  2:711112/151‑1 (MQ=255)                                                                                                                                  ccGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGTGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACg              <  1:654702/151‑1 (MQ=255)                                                                                                                                              gCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGGCCTTg  <  2:448198/151‑1 (MQ=255)                                                                                                                                               cGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCTCCCCCGAGGGGGGAGGGGGCCGGATTGTGCAAATATTGTTAACCCAGCAACAAACAAGCCCCAACAGCCCCTAACCCTTTCCTTGCGATGGGTGGGACGGGTCAGACCtt   >  2:478583/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTG  >  NC_000913/1351043‑1351333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑