Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913287,1720GC45.6% 50.6 / 21.5 47P128P (CCG→CCC) yagHCP4‑6 prophage; putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (7/18);  new base C (21/0);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 2.24e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

ACGCCGACGGTAAATTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACC  >  NC_000913/287024‑287322
                                                                                                                                                    |                                                                                                                                                      
aCGCCGACGGTAAATTCTGGCTGCTCTACACCTACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTc                                                                                                                                                      <  2:394443/151‑1 (MQ=255)
        ggTAAATTCTGGCCGCTCTACACCGACGAGAAGATTGTCGACTCGACGTGGAAAAACGTCCTCATCTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATTGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCTTGTTcc                                                                                                                                              <  2:631777/151‑1 (MQ=255)
         gTAAATTCTGGCTGCTCTACCCCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCa                                                                                                                                             <  2:488054/151‑1 (MQ=255)
           aaaTTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCac                                                                                                                                            <  2:119058/150‑1 (MQ=255)
             aTTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCacgac                                                                                                                                         <  2:674120/151‑1 (MQ=255)
                  ggCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCTCCCCGTTcc                                                                                                                                              >  1:422255/1‑141 (MQ=255)
                   gctgctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGAt                                                                                                                                   <  2:674630/151‑1 (MQ=255)
                     tgctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCTCCCCGTTTCACCACGACGAAgg                                                                                                                                 >  1:544320/1‑151 (MQ=255)
                       ctctACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCCGTTTCACGACGACGAAGGcc                                                                                                                               >  1:623952/1‑151 (MQ=255)
                           acacCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCa                                                                                                                            <  2:140416/150‑1 (MQ=255)
                               cGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCGCCCCGGTCCACCGCGAAGAAGGGCGCAAATa                                                                                                                        >  2:13737/1‑150 (MQ=255)
                                gACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCTCCCCGTTCCACGACGACGATGGGCGCAAATa                                                                                                                        >  1:156324/1‑149 (MQ=255)
                                     gAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTatat                                                                                                                  <  2:360524/150‑1 (MQ=255)
                                      aaGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGGCCCCTCCCCGTTCCACGACGACGATGGCCGCAAAAACTATATc                                                                                                                 >  2:586792/1‑150 (MQ=255)
                                      aaGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCt                                                                                                                <  1:148713/151‑1 (MQ=255)
                                            gTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCTCCCCGTTTCACCACCACGATGGCCGCCAATACTATATCTACCGcc                                                                                                          >  1:725020/1‑151 (MQ=255)
                                              cGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGCCCCCGTTCCCCCACCACCCTGGCCCCCAATAACTTATCCACCGccc                                                                                                         >  2:284997/1‑150 (MQ=255)
                                                  tCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTTACCCCTCCCTGTTCCACGACGACCATGGCCGCAAAAAATATATCTACCGCCCGTggg                                                                                                    >  1:804494/1‑151 (MQ=255)
                                                       gTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCTCCCCCTTCCACCACCACGACGGCCCCAAAAACTATAACCACCGCCCCgggggg                                                                                                  >  2:312964/1‑148 (MQ=255)
                                                        tGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCCCCCCGTTCCCCCACGCCGACGGCCGCCAAAAATATATTTACCCCCCCCggggg                                                                                                  >  2:538962/1‑147 (MQ=255)
                                                           aaaaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTTACCCCTCCCTGTTCCACGACGACGATGGCCGCAAAAACTATATCTACCCCCCCTGGGGGGCGCGcc                                                                                           >  1:198910/1‑151 (MQ=255)
                                                           aaaaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGTCCCCTCCCCGCTCCCCGACGACGACGGCCGCCAATACTATATCTACCCCCCCCGGGGGGCGCGcc                                                                                           >  2:6297/1‑151 (MQ=255)
                                                              aaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCCTCCCTGTTCCCCGACGACGATGGCCGCCAATACTATATCTACCCCCCGGGGGGGCCGCGccacc                                                                                        >  1:372988/1‑151 (MQ=255)
                                                               aCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTTACCCCCCCCCGCTCCCCCACGACCACGGCCGCAAAAACTATAACTACCCCCCGCGGGGGGCCCGccccc                                                                                        >  2:578451/1‑150 (MQ=255)
                                                                  gCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTTACCCCCCCCTGTTCCCCGACGACGGTGGCCGCCAAAACTATATTTAACGCCCGTGGGGGCCGCGCccccac                                                                                      >  1:709013/1‑149 (MQ=255)
                                                                  gCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAg                                                                                    <  1:571691/151‑1 (MQ=255)
                                                                      cAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGCTTGACCCCTCCCCGTTTCACGACGACGATGGCCCCCAATACTAAATCTACCCCCCCGGGGGGGCGCGCCACCACAGCaa                                                                                 >  1:716878/1‑150 (MQ=255)
                                                                        aCTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAAccc                                                                              <  1:774169/151‑1 (MQ=255)
                                                                         cTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTTACCCCTCCCTCTTCCCCGACGACGAAGGGCGCCAAAACTATATCTACCGCCCCCGGGGGGCGCGCCACCCCAGCAAccc                                                                              >  2:317326/1‑150 (MQ=255)
                                                                          ttCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTTACCCCTCCCTCTTCCCCCACGACGCTGGCCGCAAATACTATATCTACCGCCCGCGGGGGCCGCGCCACCACACCAAccc                                                                              >  2:238086/1‑149 (MQ=255)
                                                                              tCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCCTCCCCGTTCCACGACGACGATGGCCGCCAATACTATATCTACCCCCCCCGGGGGGCGCGCCCCCACAGCAACCCCcac                                                                          >  2:792719/1‑149 (MQ=255)
                                                                                  cACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGCTTGACCCGGCCCCGTTTCACGGCGACGATGGGCGCAAAAACTATAACCACCGCCCCTCGGGGGCGCACCCACCCAGGAAACCGCccaaaa                                                                      >  2:298746/1‑147 (MQ=255)
                                                                                             ccATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTAc                                                          <  2:130900/150‑1 (MQ=255)
                                                                                             ccATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTAc                                                          <  1:317352/150‑1 (MQ=255)
                                                                                               aTCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAgg                                                       <  1:785699/151‑1 (MQ=255)
                                                                                                  gAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGATTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGc                                                      <  1:705662/149‑1 (MQ=255)
                                                                                                    ggggCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGtt                                                   <  1:419853/150‑1 (MQ=255)
                                                                                                      ggCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGGCCCGTCCCCGTTCCACCACGACCATGGCCGCAAATACTATATCTACCGCCCCTGGGGGCCGCGCCACCCCAGCAAACCCCACAACACCATCCTGTTTCAgggggt                                                   >  1:626728/1‑144 (MQ=255)
                                                                                                       gCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGAACCCCCCCTGTTCCACCACCACGATGGCCGCAAAAAATATATCTTCCCCCCCCGGGGGCCGCCCCCCCACAGCAAACCGCCCCACACCACCGTGTTACAAGCGttt                                                  >  1:82159/1‑148 (MQ=255)
                                                                                                          aTGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGAccc                                             <  1:390267/150‑1 (MQ=255)
                                                                                                                cgagcCAATCCCGATGGGCAACGGCGGGTTTTACCCCTCCCCGTTCCCCCACGACGATGGCCGCCAATACTATATCTACCCCCCCTGGGGGCCGCGCCACCACAGCAACCCCCACAACACCATCGTGTTACAGGCGGTTTACCCCCCGAcc                                      >  1:417352/1‑151 (MQ=255)
                                                                                                                 gagcCAATCCCGATGGGCAACGGCGGGGTTGGCCCGTCCCCGGTGCCCCACCACGGTGGCCGCAAAAAATATATTTACCCCCCCCGGGGGGCGCGCCCCCACAGGCAACCGCACAACACCCCCGGGTTACAGGCGGTTGGCCCCCCGAcc                                      >  2:703998/1‑150 (MQ=255)
                                                                                                                            gATGGGCAACGGCGGGTTTTACCCCTCCCCCTTCCACGACGACGATGGCCGCCAAAAATATATCTACCCCCCCCGGGGGGCGCGCCACCCCCGCAACCCGCCCAACACCCCCGTGTTACAGGGGTTTTACCCCCCGACCCGccccccctc                           >  2:699492/1‑142 (MQ=255)
                                                                                                                                          ggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCaaaa             >  1:755644/1‑150 (MQ=255)
                                                                                                                                               gACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGt       >  1:868574/1‑151 (MQ=255)
                                                                                                                                                aCCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGtt      <  1:750606/151‑1 (MQ=255)
                                                                                                                                                 cccTTCCCTGTTCCACGACGCCGATGGCCGCAAATACTATACCTCCCGCCCGT‑GGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTa    <  1:539012/151‑1 (MQ=255)
                                                                                                                                                    gTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTAcc  >  2:499094/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ACGCCGACGGTAAATTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACC  >  NC_000913/287024‑287322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑