Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	48.9%	65.0 / 11.7	47	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/22);  new base G (23/0);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 2.02e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354138‑354437                                                                                                                                                        |                                                                                                                                                        aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGt                                                                                                                                                         <  2:693889/151‑1 (MQ=255) aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGt                                                                                                                                                         <  1:714974/151‑1 (MQ=255)    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTg                                                                                                                                                        <  2:684245/149‑1 (MQ=255)     gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGaaa                                                                                                                                                     <  1:239789/151‑1 (MQ=255)                       cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGC‑T‑GAAAggg                                                                                                                                    >  1:642153/1‑148 (MQ=255)                        ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGC‑T‑GAAAGGGCAgg                                                                                                                                >  1:32253/1‑151 (MQ=255)                          ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGC‑T‑GAAAAGGCAgggg                                                                                                                              >  1:515620/1‑149 (MQ=255)                                cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGGGC‑C‑GAAAGGGCAgggggggggg                                                                                                                        >  2:437988/1‑149 (MQ=255)                                    aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGC‑T‑GAAAGGGCAggtggcggggggg                                                                                                                     >  1:191362/1‑148 (MQ=255)                                    aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGAAGGGC‑C‑GGAAggggcggggggggggggg                                                                                                                     >  2:420391/1‑135 (MQ=255)                                          gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGC‑T‑GGAAGGGCAggtgggggggggggtt                                                                                                                  >  1:115681/1‑142 (MQ=255)                                          gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGCAAGGGCGC‑T‑GAAAGGGGGGGGGGGGGCGGCGgtt                                                                                                                  >  2:775482/1‑147 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTAGGGGGGGGGGAAAGA‑GGGC‑TCGAAAGGCAAGGGGGGGGGGGGGTTTTGCATTc                                                                                                           >  1:205848/1‑150 (MQ=255)                                                      gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGAAAGATGGGC‑T‑GGAAGGGCCGGGGGGGGGGGGGTGTGTCATTTCGCAaga                                                                                                    >  2:523986/1‑149 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGTGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                                <  1:756817/150‑1 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                             <  1:708299/151‑1 (MQ=255)                                                                  gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAAGTGCGCGG‑GAAAGGGC‑GGGGGGGGGGGGGGGTGTCTTTCCGCAAGAGGGGAGCAGTCTgg                                                                                      >  2:448553/1‑151 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                                  <  1:634933/151‑1 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGC‑T‑GAAAGGGCAGGGGGCGGTGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                          >  1:320906/1‑150 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGC‑T‑GGAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGATGTgg                                                                       >  2:860030/1‑149 (MQ=255)                                                                                     ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGC‑GGGTGGGGGGAAAGATGCGC‑C‑GCAAGGGCGGGGGGCGGCGGCGTTTGTCTTTCCGAAAGAGAGGGGCAGGCTGGAAAATCGTGGTGTgggg                                                                     >  2:172324/1‑147 (MQ=255)                                                                                      gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc                                                                  <  2:625024/151‑1 (MQ=255)                                                                                      gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGC‑T‑GGAAGGGGAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGGTGTGGGGCAc                                                                  >  2:375730/1‑151 (MQ=255)                                                                                          cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGC‑T‑GAAAGGGCAGGGGGGGGGGGGGGTTGTCCTTCCGGAAGAGAGCGACAGGCTGGGAGAACGGGATGTGGGGCACTCg                                                               >  2:450959/1‑150 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                             <  2:515570/151‑1 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGc                                                              >  1:652019/1‑150 (MQ=255)                                                                                               gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                         <  1:362469/151‑1 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                        <  1:788531/151‑1 (MQ=255)                                                                                                  tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                       <  1:206882/150‑1 (MQ=255)                                                                                                  tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                       <  2:837812/150‑1 (MQ=255)                                                                                                   gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                     <  2:54137/151‑1 (MQ=255)                                                                                                     agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGC‑T‑GAAAGGGCGGGGGGCGGGGGGGGTTGTTATTTCGAAAGAGAACGACAGTCTGGGAGAGCGGGGAGGGGCGCACTCGCAAGaggagg                                                     >  1:278177/1‑149 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                              <  2:891629/151‑1 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGC‑C‑GGAAGGGGAGGGGGGGGGGGCGTTTTTCATTCCGAAAGAGAGCGACAGGGTGGAAGAACGGGGTGTGGGGCACTCGCAAGAGAAgggggatat                                              >  2:293184/1‑144 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCt                                        <  1:380153/151‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGC‑T‑GAAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGCGCACTCGCCAGAGAAGGCGATTATGgcg                                          >  1:558883/1‑149 (MQ=255)                                                                                                                 cAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGAAGCGC‑T‑GAAAGGGCAGGAGGCGGTGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGGGCCCACTCAAGAGAAGGCGATTATGGGGCtg                                       >  1:616552/1‑151 (MQ=255)                                                                                                                    ccATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGC‑T‑GAAAGGGCAGGGGGGGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCCCTCGCCAGAGAAGGCGGTTATGGCGgtggtg                                    >  2:696248/1‑151 (MQ=255)                                                                                                                     cATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   <  2:22937/151‑1 (MQ=255)                                                                                                                        ccGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                                <  2:297466/151‑1 (MQ=255)                                                                                                                              gTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGAAGCGC‑T‑GAAAGGGGCGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGAAAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGGGGGCAGcc                             >  2:377169/1‑148 (MQ=255)                                                                                                                                 gCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                       <  1:475381/151‑1 (MQ=255)                                                                                                                                    gAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa                    <  1:623957/151‑1 (MQ=255)                                                                                                                                     aaGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                   <  1:383130/151‑1 (MQ=255)                                                                                                                                      aGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGTGGTGGCGTTTGTCATTCCGAAAGAGGGCGACAGTCTGGAAGATCGTGATGTGGCGCGCTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                        <  2:333331/145‑1 (MQ=255)                                                                                                                                            ggt‑ggtTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc            >  1:316879/1‑151 (MQ=255)                                                                                                                                                    ggggTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg      <  1:752407/150‑1 (MQ=255)                                                                                                                                                    ggggTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg      <  1:448600/150‑1 (MQ=255)                                                                                                                                                       gTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  2:890614/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                        ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGC‑T‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354138‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑