| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 535,901 | 0 | T | G | 48.3% | 70.1 / 12.4 | 60 | G64G (GGT→GGG) | hyi | hydroxypyruvate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (8/23); new base G (28/1); total (36/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGG > NC_000913/535758‑536044 | tttCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAGTggg > 2:863606/1‑151 (MQ=255) tctcGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGATGggcg > 2:310757/1‑151 (MQ=255) ctcGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTgggggg > 2:337305/1‑151 (MQ=255) cGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGggcggc < 2:153272/150‑1 (MQ=255) cGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGggcgg > 1:69748/1‑149 (MQ=255) ccGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGGCTGGGGGGCgggg > 2:180779/1‑151 (MQ=255) ttGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAgt < 1:389057/150‑2 (MQ=255) agaAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCgg < 2:895463/151‑1 (MQ=255) agaAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAATGGGCGGCggggggggggg > 2:7924/1‑144 (MQ=255) gaAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGGGGGGGAGGGCgg > 2:325478/1‑150 (MQ=255) aGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTAtt < 1:279669/151‑1 (MQ=255) gCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTggggggggggggggggggg > 2:708036/1‑139 (MQ=255) cTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGGGGCgggggggggggg > 2:707520/1‑138 (MQ=255) cgcAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAATGGGGGGCggggggggggggggttgcc > 1:366389/1‑135 (MQ=255) gcAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGGGGGGGAGGGGGGGATTggc > 2:604999/1‑146 (MQ=255) gtgtGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑tt < 2:396237/151‑1 (MQ=255) ggTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGGGGCGGGGGGGGGCGGGATTGCCTGTA‑t > 2:617930/1‑146 (MQ=255) cgTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTgaagaa < 1:544950/151‑1 (MQ=255) cgTAGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTgaagaa < 1:455886/151‑1 (MQ=255) gTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGaagaag < 2:272948/151‑1 (MQ=255) gTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGCGGGGGGGGGCGGGATTTCCTGTA‑TTTCTGGCCggggag > 1:523345/1‑143 (MQ=255) tGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGCGGGGGGGGGCGGGATTTCCTGTA‑TTGCTGGCCGTGaagaag > 1:701745/1‑149 (MQ=255) tttATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGGGGCGGGGGAGTGCGGGAATGCCCGTT‑TTCCTGGCCGTGaagaag > 1:513742/1‑145 (MQ=255) tATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCg < 2:195097/150‑1 (MQ=255) gTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGAt < 2:70943/151‑1 (MQ=255) gTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGGGGCGGGGGGGGGGGGGATTGCCTGTT‑TTTCTTGCCGGGGAGAAGAGGTTCgggg > 2:824827/1‑149 (MQ=255) tCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGAGTGGGCGGGGGGGGGGAGGGGGATTGCCTGTA‑TTTCTGGCCGGGAGGAAGAGgttt > 2:22628/1‑142 (MQ=255) cTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGGGGGGGGGGGGGGGATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGGTTTGGGGTGGCg > 1:422375/1‑150 (MQ=255) aTGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTagc < 2:320617/151‑1 (MQ=255) gACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTagcag < 2:141951/151‑1 (MQ=255) gACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTagca < 2:471642/150‑1 (MQ=255) cGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGGGGGGGGGGGCGGGATTGCCTGTA‑TTTCTGGCCGGGAAGAAGAGGTTCGGGGTGGCGGagcagca > 1:815093/1‑148 (MQ=255) cGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGGGCGGGATTGCCTGTA‑TTTCTTGCCGGGAAGAAGAGGTTCGGGGTgggg > 1:357081/1‑138 (MQ=255) gaagaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACCGGGGGGGGGGGGGGGGCGGGATTGCCTGTA‑TTTCTGGCCGGGGAGAAGAGGTTCGGGGGGGGGGAGCAGCAGCGATTCggt > 1:121654/1‑149 (MQ=255) gaagaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTGCGGGATTGCGTAGCAGCAGCGATTCggt < 2:819541/151‑3 (MQ=255) aagaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGGGTGCGGGATTGCCTGTA‑TTTCTGGCCGTGAAGAAGAGGTTTGGGATGGCGGAGCAGCAGCGATTCGtt > 1:243191/1‑150 (MQ=255) gaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGGGGCGGGGGGGGGGGGGATTGGCTGTA‑TTTCTGGGCGTGGAGAAGAGGTTCGGGGGGGCGGAGGAGCAGCGGTTTGTTAt > 1:31758/1‑150 (MQ=255) ttAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGGGGCGGGGGGGTGCGGGATTGCCTGTA‑TTTCTGGCCGTGGAGAAGAGTTTTGGGGTGGCGTAGCAGCAGCGATTTGTTATTccc > 1:474034/1‑151 (MQ=255) tAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGCGGGGGGGGGGGGCGGGATTGCCCGTA‑TTTCCGGGCGGGGAGAAGAGGTTTGGGGGGGGGGAGGAGCAGCGATTCGTTATGgcc > 2:756079/1‑150 (MQ=255) aaCATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTgc < 1:459225/151‑1 (MQ=255) aaCATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGGGGCGGGGGGGGGCGGGATTTCCTGTA‑TTTCTGGCCGTGAAGAAGAGGTTCGGGATGGGGGAGCAGCAGCGGTTCGTTATGCCCCTg > 1:497746/1‑150 (MQ=255) cATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGGGGGGGATTTCCTGTA‑TTTCTGGCCGTGGAGAAGAGGTTTGGGGTGGCGGAGCAGCAGCGATTTGTTATTCCCgcg > 1:7136/1‑146 (MQ=255) aTGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGATTTGGGGGCGGGGGGGTGTGGGTATGTCTGTA‑TTTTCCGGCGCGGAGAAGAGGGTCGGGGTGGGGTAGCAGCAGCGGCTCGTTATGcccct > 1:381405/1‑144 (MQ=255) gtgCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTgg < 2:665389/151‑1 (MQ=255) gCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGt < 1:303159/151‑1 (MQ=255) gCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGt < 1:302186/151‑1 (MQ=255) gCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTTGGCGGCGGGGGGGGGGGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGGTTCGGGGTGGCGGAGCCGCAGCGATTCGTTATTCCCGTGCGCCggg > 1:807096/1‑150 (MQ=255) cTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGGGGCGGGGGGGGGCGGGATTGCCTGTA‑TTTCTGGGCGGGGAGAAGGGGTTTGGGGGGGGGGAGGAGCAGCGATTTGGTTTGGCCGTgcggcggg > 2:560869/1‑144 (MQ=255) ggCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGTCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGtaa < 1:636258/150‑1 (MQ=255) gCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGACTGGGCGGCGGGGGGGGGCGGGATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGGTTCGGGGTGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGgaa > 1:530311/1‑149 (MQ=255) aGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGGATTGGGGGGGGGGGGGGGGGGGATTGCCTGTT‑TTCCTGGCCGGGGAGAAGAGGTTTGGGGGGGGGGAGCAGCCGCGAGTTGTTTTGTCCCTGCGCTGGGGGATaaa > 2:1057/1‑150 (MQ=255) aGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGGGGCGGGGGGGGGCGGGAGTTACTGTA‑TTCCTGGGCGGGGAGAAGAGTTTTGGGGTGGGGGAGCAGCAGAGATTCGTGATGCCCGTGCGCGGGGGGATaaa > 2:819716/1‑150 (MQ=255) aGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATaaaa < 1:44787/151‑1 (MQ=255) aGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGAATGGGCGGGGGGGGGAGGGGGGATTTCCTGTATTTCCGGGCCG‑GGAGGAGAGGGTTGGGGTGGCGGTGGAGCAAGGATTCGTTATGACCCTGCGCCGGGGaaaaaa > 1:188157/1‑150 (MQ=255) gTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGCTGGGAGGCGGGGGAGGGGGGGAATGCCTTTA‑TTCCTGGCCGTGGAGAAGAGGTTCGGGGTGGGGGGGCAGCAGCGATTCGTTTTGGCCCGGGGCTGGGGGATaaaa > 2:625459/1‑150 (MQ=255) cATACGCTGCACAATTTACCGGCGGGTGACTGGGGGGCGGGGGGGGGCGGGATTGCCTGTA‑TTTCTGGCCGTGAAGAAGAGTTTCGGGGTGGCGGAGCAGCAGCGATTTGTTTTGCCCGTGCGCTGGGGGATAAAAAAATTAACTGTCTgg > 1:159367/1‑151 (MQ=255) cGCTGCACAATTTACCGGCGGGTGACTTGGCGTCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCCTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGTGTAATAAAAAAATTAACTGTCTGGTCgg < 2:333092/151‑1 (MQ=255) cGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCgg < 1:462744/151‑1 (MQ=255) cTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGTGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGt < 1:841823/150‑1 (MQ=255) ccGGCGGTTGACGGGGCGGCGGGGGAGTGCGGTATGGCCGGTA‑TTCCTGGCCGTGAGAAAGAGTTCCGGAATGCGTTACCAGCAGCGATTCGTTATGCCCGTGGGCTGGGTAAT‑AAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTgg < 2:257477/151‑1 (MQ=255) | TTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTA‑TTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGG > NC_000913/535758‑536044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |