Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,7740GA45.8% 46.9 / 20.3 48intergenic (‑388/+160)sufA/ydiHFe‑S cluster assembly protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (19/7);  new base A (0/22);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 7.36e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCATT  >  NC_000913/1764638‑1764924
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aaGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaaa                                                                                                                                          <  2:116207/151‑1 (MQ=255)
 agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAg                                                                                                                                         <  1:789997/151‑1 (MQ=255)
     aTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTaa                                                                                                                                     <  1:490150/151‑1 (MQ=255)
           aaaaaaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc                                                                                                                               <  1:493997/151‑1 (MQ=255)
            aaaaaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgccg                                                                                                                              >  1:759218/1‑151 (MQ=255)
               aaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGttt                                                                                                                           >  2:369957/1‑151 (MQ=255)
                        gttaaaaaaaaaaaaaCCCC‑TTAGGTCTGGCCGACAAGGTCTAAAGGGTTTCCCCCCCACCGGGCGCTTATCCCCGGCCCTCCCAGTGGTTTAGCTTTGGAAGGGGCAATAAAAATAATCTAAAAAAGCTAACCCGCCGTTTTAaca                                                                                                                      <  2:206299/141‑1 (MQ=255)
                           aaaaaaaaaaaaaACCC‑TTAGGTCTGTGCGACACAGGCTAAAGGGTCTCTCCCCCACCCGGCGCTTACCCCCGGCACTCTCAGTGGTTGATCTCTTGAAGGGAAAAAAAAAAAAATCTAAAAAAGCTAACCCGCCGTTTTAACACAAACt                                                                                                                <  2:794244/147‑1 (MQ=255)
                             aaaaaaaaaaaCCCC‑TTAAGTTTGCGCAACACAGTCTAAAGGGTTTCTCCCCCATCCGGCCCTTATCCCCGCCCCTCTCGGGGCTTTGGCTTTTAAGGGGACAATAAAAAAAACCTAAAAAACCTACCCCGCCGTTTTAACACAAAc                                                                                                                 <  2:730992/146‑1 (MQ=255)
                               aaTAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAt                                                                                                           >  2:85264/1‑151 (MQ=255)
                                  aaGAAACCCC‑TAATTTCTGTCCAACACAGGTTTAGGTTTTCCTCCCCCACCCGGCCCTTTTCTCCGCCCCCCTCAGTGGCTTAGTTTTTGAGGGGGCAAAAAGTTTATTCCAATAAATCAAACCCGCCGTTTTAACACAAACTGCGATTa                                                                                                         <  2:863543/150‑1 (MQ=255)
                                   aaaaaaCCCTTTAATTCTGTGCGACACGGGTTTAGGGTTTTCTCCCCCACCCGGCGCTTATCCCCGGCCCTCTCAGTGGTTTAGCTCTTGAAGGGGCAAAAAAAATAATCCCATAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTa                                                                                                         <  1:710094/148‑1 (MQ=255)
                                     aaaaCCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtat                                                                                                     >  2:576540/1‑151 (MQ=255)
                                         ccc‑TTAAGTCTGGGCGACACAGGCTTAGGGTTTTCAACCCCATCGGGCGCTTATCTCCGCCACTCTCGGTGCTTTAGTTTTTGAAGGGGCAATAAGAATAATTTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGtattat                                                                                                  <  2:587440/150‑1 (MQ=255)
                                               aaGTCTGTGCGACACAGGTTTAAGGGTTTCTCCCCCACCCGGCGTTTATCCCCGCCCCTCTCGGTGGTTTAGCTTTTGAAGGGGCAATAAAAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTAtttt                                                                                               <  2:780647/148‑1 (MQ=255)
                                                aGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGa                                                                                            >  2:356744/1‑150 (MQ=255)
                                                      tgCGACACAGGTTTAGGGTTTCCTCCCCCACCCGGCGTTTACCCCCGCCCCTCTCAGGGGCTTAGCTTTTAAAGGGGAAAAAAGAATAATCTCAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACaa                                                                                        <  1:221838/148‑1 (MQ=255)
                                                      tgCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAatat                                                                                     >  1:219550/1‑151 (MQ=255)
                                                        cGACACAGGTTTAGGGTTTTCTCCCCCATCCGGCGTTAATCTCCGGCACTCTCAGTGGTTTAGCTTTTGAAGGGGAGATAAAAATAATTTCAAAAAGTTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCa                                                                                   <  2:665049/151‑1 (MQ=255)
                                                               ggCTTAAGGTTTTCTCCCCCATCCGGCTTTTATCTCCGCCACTCCCAGGGGTTTAGCTTTTGAAGGGGCAAAAAAAATAATCTCAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGt                                                                             <  2:753838/150‑1 (MQ=255)
                                                               ggCTTAAGGTTTTCTACCCAACCCGGCTCTTACCCCCGCCACTCTCGGGGGCTTAGCTGTTGAAGGGGGAATAAAAATAATCTTAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAatat                                                                                     <  2:345743/142‑1 (MQ=255)
                                                               ggCTTAAGGTTTTCCCCCCCATCCGCCCCTATTCCCGGCCATTCTAAGGGGTTAACCTTTGAAAGGGGCAATAAAAATATTCTAAAAAACCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCgg                                                                              <  1:303815/149‑1 (MQ=255)
                                                                gtttAAGGGTTTCTCCCCCATCCGGCGCTAACCCCCGGCCCTCTCAGCGGTTTTGCTTTAGAGGGGGAAAAAAAAATAACCTAATAACGCTCACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGc                                                                                <  2:155158/144‑1 (MQ=255)
                                                                 cTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGt                                                                             >  2:388450/1‑148 (MQ=255)
                                                                   tAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGAt                                                                         >  2:125984/1‑150 (MQ=255)
                                                                      gtttttCTCCCCCACCCGGCGTTTATCCCCGGCACTCTCAGTGGTTTAGCTTTAGAAGGGGAGAAAAAAATAATCTAATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATa                                                                        <  2:736924/145‑1 (MQ=255)
                                                                          ttCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGt                                                                  >  2:152683/1‑150 (MQ=255)
                                                                           tcccccccACCCGGCGATTATCCCCGGCACTCTCGGTGTCTAGTCTTTGAAAGGGGGAAAAAAAATAATCTAAAAAAGATACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGt                                                                  <  2:184677/145‑1 (MQ=255)
                                                                             tACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTAtt                                                               >  2:843234/1‑150 (MQ=255)
                                                                              cccccACCCGGCGCTTATCCCCGCCCCTCTCGGTGGCTTAGCTCTTGAAGGGGCAAAAAAAAAAATCTAAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTAtt                                                               <  1:857752/148‑1 (MQ=255)
                                                                               ccccccccGGCCCTTATCTCCGGCCCTCTCAGTGTTTTAGCTTTTAAGGGGGAAATAAAAATAATCTCAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTAtt                                                               <  2:898973/142‑1 (MQ=255)
                                                                               ccccACCCGGCGCTTATCCCCGGCACTCTCAGTGGCTTAGCTTTTAAAGGGGCAATAAAAATAATCTCATAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTaa                                                             <  2:334002/150‑1 (MQ=255)
                                                                                   acccGGCTCTTATCTCCGGCCCTCTCGGTGGTTTAGCTTTTGAGGGGGAGAAAAAAATAATCTCATAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAgg                                                        <  2:449251/149‑1 (MQ=255)
                                                                                    cccGGCGCTTACCCCCGCCCTTCTTAGTGGCTTAGCTCTTGAAGGGGCGATAAAAAAAATCTAACAAAGAAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGt                                                       <  1:597027/150‑1 (MQ=255)
                                                                                       ggCGTTTATCCCCGGCCCTCTAGGTGGATTAGCTCTTGAAGGGGAAAAAAAAATAATCTCAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCAt                                                    <  1:136547/151‑1 (MQ=255)
                                                                                          gcTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAg                                                                   >  2:873692/1‑133 (MQ=255)
                                                                                            ttATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAAc                                               >  2:512772/1‑151 (MQ=255)
                                                                                                  ccGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGAc                                         >  2:76533/1‑151 (MQ=255)
                                                                                                    gccACTCTAGGTGTTTTAGCTTTTGAGGGGGAGAAAAAAATAATCTAAAAAAGATAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTg                                            <  2:555185/144‑1 (MQ=255)
                                                                                                      cACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACgg                                       >  1:630328/1‑149 (MQ=255)
                                                                                                           tcAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTa                                 >  2:24843/1‑150 (MQ=255)
                                                                                                               tGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATcc                             >  2:373215/1‑150 (MQ=255)
                                                                                                                     aGCTTTTGAAGGGGCAAAAAAAATAATCTCATAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTc                        <  1:400048/149‑1 (MQ=255)
                                                                                                                     aGCTCTTGAAGGGGCGATAAGAATACTCTCATAAAGCTAAGCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCg                       >  2:825869/1‑150 (MQ=255)
                                                                                                                             aaGGGGCGAAAAAAATAATCACAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGc                <  1:615778/149‑1 (MQ=255)
                                                                                                                             aaGGGGCAAAAAAAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTg                  <  2:843257/147‑1 (MQ=255)
                                                                                                                              aGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGa              >  2:567636/1‑150 (MQ=255)
                                                                                                                                        aGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCAt   >  2:202010/1‑151 (MQ=255)
                                                                                                                                         gAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCAtt  >  1:219028/1‑151 (MQ=255)
                                                                                                                                         |                                                                                                                                                      
AAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCATT  >  NC_000913/1764638‑1764924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑