| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,678,039 | 0 | T | G | 47.7% | 42.9 / 19.6 | 44 | N108T (AAC→ACC) | yphE | putative sugar ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (5/18); new base G (21/0); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC > NC_000913/2677891‑2678189 | caGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑ACCGCGGCAGGGGgtt > 2:732873/1‑151 (MQ=255)caGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGttt < 2:890064/151‑1 (MQ=255) gctgctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGgtt > 2:270942/1‑149 (MQ=255) ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGGTTTTCGcc > 1:893314/1‑151 (MQ=255) ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCCAGGCAGAGGGTTTTCGcc > 2:505835/1‑151 (MQ=255) cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGc < 2:805575/151‑1 (MQ=255) cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTTAg > 1:794565/1‑150 (MQ=255) cGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑AACGAGGCAGAGGGTTTCCGCCACTGTCCGCCCTTccacc > 1:373873/1‑151 (MQ=255) gTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTccccc > 1:773799/1‑150 (MQ=255) tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCG‑ACCGAGGCCGAGGGTTTTCGCCACTCTCAGCCCCTccccc > 2:422668/1‑148 (MQ=255) aaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGAc < 2:485300/149‑1 (MQ=255) aCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTa < 1:820331/151‑1 (MQ=255) aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑ACCCACGCAGAGGGTTTTCCCCCCTTCCACCCCTTCCCCCCGCCtt > 2:397494/1‑147 (MQ=255) cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCGT‑ACCGGGGGAGAGGGTTTCCTCCACCGTCAGCCCCTCCTCCATACATaaaccctcat > 1:781563/1‑142 (MQ=255) cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATa < 1:726145/151‑1 (MQ=255) gACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTG‑ACCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCACCACACCTAACTCCTGATaaa > 2:244543/1‑150 (MQ=255) tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCG‑ACCCGCGCAGGGGGTTTCCCCCCCCGTCACCCCCTCCCCCCCACTTAACTCCTTGTAAAcc > 2:750957/1‑149 (MQ=255) aCGTCACCGCCAAGCGCCGGTAAGCAACTTTGGGCATCCTGCGCCTTTTGCAGGTAATCAATCATGCCGTTGCGGCGGGACCACTG‑ACCGAGGCAGAGGTTTTCCGCCTCTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAAcc < 1:665548/147‑1 (MQ=255) aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTT‑ACCGCGGCAGAGGTTTTCCGCCCCCGTCAGCCCTTCCACCCGACTTAACTCCTGATAAAACCCGCGCAcc > 1:807708/1‑151 (MQ=255) cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCG‑ACCCAGGCAGAGGGTTTTCGCCCCCGTCCGCCCTTCCACCCGACTTTACTCCCGATAAAACGCGCGCccccccc > 2:732165/1‑150 (MQ=255) cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCa < 1:772758/151‑1 (MQ=255) cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCa < 2:503902/151‑1 (MQ=255) aGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑AACCAGGGAGAGGGTTTTCGCCCCCGTCCGCCCCTCCCCCCCACCTAACTCCCGATTAAACCCGCGCCCCCCCCGtt > 2:204411/1‑151 (MQ=255) ccTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGCCCCGCGCCGGGGGTTTTCC‑CCCCCGTTACCCCTTCCCCCAGCCTTTAATCCTGCTTAAACGCCCGCGCCCCCAGCTcc > 2:251157/1‑148 (MQ=255) gTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCgg < 2:231719/150‑1 (MQ=255) tAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCT‑ACCGAGGCAGAGGGTTTTCTCCCCCTTCAGCCCTTCCCCCAGACTTTACTCCTGATAAAACCCGCGCCCCCCCCGTTCCGCgg > 1:700495/1‑149 (MQ=255) gCACCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACg < 2:876130/150‑1 (MQ=255) aaCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTG‑ACCCAGGCAGAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCAAACCTTAATCCTGATAAAAAGCGCGCACCCCCCCGTCAGCGGCAc > 2:360914/1‑147 (MQ=255) ggCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCa < 2:578282/149‑1 (MQ=255) aTCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTa < 2:19147/151‑1 (MQ=255) cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCCGACTTAACTCCCGATAAACCGCGCGCACCCCCCGTTCAGCGGCACGGCGGGGCCGCGTAGc > 1:403381/1‑150 (MQ=255) gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCt < 1:885318/149‑1 (MQ=255) gcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCG‑ACCGGGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCACACTTAACTCCTGATAAAACCCGCGCACCCCCCCTTCAGCGGCACGGCGAGGCAGCGCAGCTTCg > 2:833632/1‑150 (MQ=255) ttGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTG‑ACCGAGGGAGAGGGTTTTCGCCCCTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAAACCCGCGCACCCCCAGTTCAGCGGCACGGCGAGGCAGCGTAGCTTCGTCAcc > 1:147000/1‑150 (MQ=255) ttGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑ACCCAGGCAGAGGGTTTTCCCCACCTTCAGCCCCTCCACCCCACTTAAATCCCGATAAAACCCCCCCCCCCCCCCCTCCCCGGCCCGGCGAGGCCGCCGAGCCTCGTccccc > 2:657062/1‑150 (MQ=255) cAGGTAATCAATCATGCCGTTGCGGCGGGGGCGCTT‑ACCGCGGCAGAGGGTTTTCCCCCCCGTCCGCCCTTCCACCACACTTTACTCCCCGTGAACCCCGCCCCCCCCCCGCTCAGCGGCCCCGCGAATGAGCGCAGCCCCGTCCCCATcc > 2:250043/1‑151 (MQ=255) cAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCG‑ACCGAGGGAGAGGGTTTTCCCCACTGTTAGGCCCTCCCCCAGACTTTACTCCTGTTAAAACGCGAGCACCCCCCGTTCAGCGGGACGGCGGGTCAGCGGAGCTTTGTCAcccttc > 1:338324/1‑147 (MQ=255) ggggaagcaaccaTGCCGTTGCGGCGGGGCCACGG‑CCCGAGGCAGAGGTTTGCCGCCACTGTCAGCCCTGCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCTAGTCAGCGTAGCTGCGTCGCCTTCCa < 2:573471/144‑1 (MQ=255) gTAATCAATCATGACGTTGAGGCGGAGACACTG‑AACGAGGCGGAGGTTTTTCCCCCCAGTCGGGCCTTCCACGAGAATTAATCCCTGATAAACCGCGCGCACCCCCCCGTCCGCGGCACGGAGGGGCGGCGGAGCTTCTTCaacct > 2:629055/1‑142 (MQ=255) aatcaatcATGCCGTTGCGGCGGGGCCCCTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTCCGCCCCTCCACCAGACCTTACTCCTGGTAAACCGCCCGCACCCCCCCTTCAGCGGCACCGCGGGGCAGCGGAGCCTCTCCACCCTcc > 1:262009/1‑146 (MQ=255) tGCCGTTGTGGGGGGGCCAATA‑AAGGAGGCAGAGATTTTCCGCGATTGTCAGCACTTCCGCGAGACATAATGCCTGGTAAACCGCGCGCACCACCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTc < 2:605313/141‑1 (MQ=255) gCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGcc < 2:403355/150‑1 (MQ=255) g‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTAt < 2:515977/150‑1 (MQ=255) aCCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATcc > 1:365245/1‑151 (MQ=255) ccGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCg > 1:335683/1‑151 (MQ=255) gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc < 2:32851/150‑1 (MQ=255) gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCg < 2:848306/151‑1 (MQ=255) ttttCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGc > 2:302052/1‑151 (MQ=255) | CAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC > NC_000913/2677891‑2678189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |