Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,678,0430CT42.2% 31.5 / 26.1 45E107K (GAA→AAA) yphEputative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (8/18);  new base T (19/0);  total (27/18)
Fisher's exact test for biased strand distribution p-value = 1.10e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC  >  NC_000913/2677897‑2678189
                                                                                                                                                   |                                                                                                                                                  
ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGGTTTTCGcc                                                                                                                                                >  1:893314/1‑151 (MQ=255)
ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCCAGGCAGAGGGTTTTCGcc                                                                                                                                                >  2:505835/1‑151 (MQ=255)
         cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGc                                                                                                                                       <  2:805575/151‑1 (MQ=255)
         cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTTAg                                                                                                                                        >  1:794565/1‑150 (MQ=255)
                  cGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑AACGAGGCAGAGGGTTTCCGCCACTGTCCGCCCTTccacc                                                                                                                              >  1:373873/1‑151 (MQ=255)
                   gTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTccccc                                                                                                                              >  1:773799/1‑150 (MQ=255)
                     tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCG‑ACCGAGGCCGAGGGTTTTCGCCACTCTCAGCCCCTccccc                                                                                                                              >  2:422668/1‑148 (MQ=255)
                        aaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGAc                                                                                                                          <  2:485300/149‑1 (MQ=255)
                         aCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTa                                                                                                                       <  1:820331/151‑1 (MQ=255)
                            aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑ACCCACGCAGAGGGTTTTCCCCCCTTCCACCCCTTCCCCCCGCCtt                                                                                                                        >  2:397494/1‑147 (MQ=255)
                                   cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCGT‑ACCGGGGGAGAGGGTTTCCTCCACCGTCAGCCCCTCCTCCATACATaaaccctcat                                                                                                              >  1:781563/1‑142 (MQ=255)
                                   cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATa                                                                                                             <  1:726145/151‑1 (MQ=255)
                                      gACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTG‑ACCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCACCACACCTAACTCCTGATaaa                                                                                                           >  2:244543/1‑150 (MQ=255)
                                         tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCG‑ACCCGCGCAGGGGGTTTCCCCCCCCGTCACCCCCTCCCCCCCACTTAACTCCTTGTAAAcc                                                                                                         >  2:750957/1‑149 (MQ=255)
                                           aCGTCACCGCCAAGCGCCGGTAAGCAACTTTGGGCATCCTGCGCCTTTTGCAGGTAATCAATCATGCCGTTGCGGCGGGACCACTG‑ACCGAGGCAGAGGTTTTCCGCCTCTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAAcc                                                                                                         <  1:665548/147‑1 (MQ=255)
                                                aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTT‑ACCGCGGCAGAGGTTTTCCGCCCCCGTCAGCCCTTCCACCCGACTTAACTCCTGATAAAACCCGCGCAcc                                                                                                >  1:807708/1‑151 (MQ=255)
                                                    cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCG‑ACCCAGGCAGAGGGTTTTCGCCCCCGTCCGCCCTTCCACCCGACTTTACTCCCGATAAAACGCGCGCccccccc                                                                                            >  2:732165/1‑150 (MQ=255)
                                                    cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCa                                                                                            <  1:772758/151‑1 (MQ=255)
                                                    cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCa                                                                                            <  2:503902/151‑1 (MQ=255)
                                                       aGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑AACCAGGGAGAGGGTTTTCGCCCCCGTCCGCCCCTCCCCCCCACCTAACTCCCGATTAAACCCGCGCCCCCCCCGtt                                                                                         >  2:204411/1‑151 (MQ=255)
                                                           ccTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGCCCCGCGCCGGGGGTTTTCC‑CCCCCGTTACCCCTTCCCCCAGCCTTTAATCCTGCTTAAACGCCCGCGCCCCCAGCTcc                                                                                       >  2:251157/1‑148 (MQ=255)
                                                              gTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCgg                                                                                   <  2:231719/150‑1 (MQ=255)
                                                               tAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCT‑ACCGAGGCAGAGGGTTTTCTCCCCCTTCAGCCCTTCCCCCAGACTTTACTCCTGATAAAACCCGCGCCCCCCCCGTTCCGCgg                                                                                   >  1:700495/1‑149 (MQ=255)
                                                                  gCACCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACg                                                                               <  2:876130/150‑1 (MQ=255)
                                                                    aaCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTG‑ACCCAGGCAGAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCAAACCTTAATCCTGATAAAAAGCGCGCACCCCCCCGTCAGCGGCAc                                                                                >  2:360914/1‑147 (MQ=255)
                                                                           ggCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCa                                                                       <  2:578282/149‑1 (MQ=255)
                                                                              aTCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTa                                                                  <  2:19147/151‑1 (MQ=255)
                                                                                 cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCCGACTTAACTCCCGATAAACCGCGCGCACCCCCCGTTCAGCGGCACGGCGGGGCCGCGTAGc                                                                >  1:403381/1‑150 (MQ=255)
                                                                                   gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCt                                                               <  1:885318/149‑1 (MQ=255)
                                                                                     gcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCG‑ACCGGGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCACACTTAACTCCTGATAAAACCCGCGCACCCCCCCTTCAGCGGCACGGCGAGGCAGCGCAGCTTCg                                                            >  2:833632/1‑150 (MQ=255)
                                                                                          ttGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTG‑ACCGAGGGAGAGGGTTTTCGCCCCTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAAACCCGCGCACCCCCAGTTCAGCGGCACGGCGAGGCAGCGTAGCTTCGTCAcc                                                       >  1:147000/1‑150 (MQ=255)
                                                                                          ttGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCG‑ACCCAGGCAGAGGGTTTTCCCCACCTTCAGCCCCTCCACCCCACTTAAATCCCGATAAAACCCCCCCCCCCCCCCCTCCCCGGCCCGGCGAGGCCGCCGAGCCTCGTccccc                                                      >  2:657062/1‑150 (MQ=255)
                                                                                             cAGGTAATCAATCATGCCGTTGCGGCGGGGGCGCTT‑ACCGCGGCAGAGGGTTTTCCCCCCCGTCCGCCCTTCCACCACACTTTACTCCCCGTGAACCCCGCCCCCCCCCCGCTCAGCGGCCCCGCGAATGAGCGCAGCCCCGTCCCCATcc                                                   >  2:250043/1‑151 (MQ=255)
                                                                                             cAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCG‑ACCGAGGGAGAGGGTTTTCCCCACTGTTAGGCCCTCCCCCAGACTTTACTCCTGTTAAAACGCGAGCACCCCCCGTTCAGCGGGACGGCGGGTCAGCGGAGCTTTGTCAcccttc                                                   >  1:338324/1‑147 (MQ=255)
                                                                                              ggggaagcaaccaTGCCGTTGCGGCGGGGCCACGG‑CCCGAGGCAGAGGTTTGCCGCCACTGTCAGCCCTGCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCTAGTCAGCGTAGCTGCGTCGCCTTCCa                                                  <  2:573471/144‑1 (MQ=255)
                                                                                                gTAATCAATCATGACGTTGAGGCGGAGACACTG‑AACGAGGCGGAGGTTTTTCCCCCCAGTCGGGCCTTCCACGAGAATTAATCCCTGATAAACCGCGCGCACCCCCCCGTCCGCGGCACGGAGGGGCGGCGGAGCTTCTTCaacct                                                     >  2:629055/1‑142 (MQ=255)
                                                                                                  aatcaatcATGCCGTTGCGGCGGGGCCCCTG‑ACCGAGGCAGAGGGTTTTCGCCCCTGTCCGCCCCTCCACCAGACCTTACTCCTGGTAAACCGCCCGCACCCCCCCTTCAGCGGCACCGCGGGGCAGCGGAGCCTCTCCACCCTcc                                                   >  1:262009/1‑146 (MQ=255)
                                                                                                           tGCCGTTGTGGGGGGGCCAATA‑AAGGAGGCAGAGATTTTCCGCGATTGTCAGCACTTCCGCGAGACATAATGCCTGGTAAACCGCGCGCACCACCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTc                                               <  2:605313/141‑1 (MQ=255)
                                                                                                            gCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGcc                                     <  2:403355/150‑1 (MQ=255)
                                                                                                                                g‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTAt                 <  2:515977/150‑1 (MQ=255)
                                                                                                                                  aCCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATcc               >  1:365245/1‑151 (MQ=255)
                                                                                                                                   ccGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCg              >  1:335683/1‑151 (MQ=255)
                                                                                                                                           gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc       <  2:32851/150‑1 (MQ=255)
                                                                                                                                           gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCg      <  2:848306/151‑1 (MQ=255)
                                                                                                                                               ttttCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGc  >  2:302052/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTG‑ACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC  >  NC_000913/2677897‑2678189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑