Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	13,865	C→T	A568V (GCG→GTG)	dnaK →	chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	13,865	0	C	T	100.0%	142.5 / NA	38	A568V (GCG→GTG)	dnaK	chaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/13);  total (25/13)

ACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTT  >  NC_000913/13727‑14011                                                                                                                                           |                                                                                                                                                   aCGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACt                                                                                                                                        >  2:540725/1‑151 (MQ=255)       aaGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAAc                                                                                                                                  <  1:147288/151‑1 (MQ=255)            gACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGc                                                                                                                               <  1:152058/149‑1 (MQ=255)             aCCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGctct                                                                                                                            >  1:340448/1‑151 (MQ=255)               cGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGctcta                                                                                                                           >  1:777587/1‑149 (MQ=255)                  aaGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGaa                                                                                                                         >  1:436431/1‑149 (MQ=255)                               tGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGc                                                                                                          >  1:535208/1‑151 (MQ=255)                                ggTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGcc                                                                                                         <  1:794966/151‑1 (MQ=255)                                   aCAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCt                                                                                                      <  2:417572/151‑1 (MQ=255)                                    cAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCt                                                                                                      >  1:272119/1‑150 (MQ=255)                                    cAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTa                                                                                                     <  2:100752/151‑1 (MQ=255)                                       aCTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCg                                                                                                  >  1:241838/1‑151 (MQ=255)                                          cGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGa                                                                                                 >  2:522090/1‑149 (MQ=255)                                               ccAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGaa                                                                                           >  2:483646/1‑150 (MQ=255)                                                        ccATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGaa                                                                                                                                    <  2:112153/100‑1 (MQ=255)                                                        ccATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGaa                                                                                                                                    >  1:112175/1‑100 (MQ=255)                                                               ctgcACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGcac                                                                          >  2:527653/1‑151 (MQ=255)                                                                  cacaGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAgg                                                                       <  2:814125/151‑1 (MQ=255)                                                                   acaGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGt                                                                      <  1:567150/151‑1 (MQ=255)                                                                                   ggTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATg                                                      >  1:688808/1‑151 (MQ=255)                                                                                     ttGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATgg                                                     >  2:50913/1‑150 (MQ=255)                                                                                      tGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGa                                                    >  2:200001/1‑150 (MQ=255)                                                                                       gaagaaGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGaa                                                   >  2:85778/1‑150 (MQ=255)                                                                                         agaagCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATc                                                <  2:365938/151‑1 (MQ=255)                                                                                            agCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGcc                                             <  2:210207/151‑1 (MQ=255)                                                                                                  cGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCcagcag                                       <  2:189023/151‑1 (MQ=255)                                                                                                  cGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCcagc                                         >  1:728546/1‑149 (MQ=255)                                                                                                   gACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCagcagc                                      >  1:281636/1‑151 (MQ=255)                                                                                                    aCAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAgcagca                                     <  1:448944/151‑1 (MQ=255)                                                                                                            ccGGCTGACGACAAAACTGCTATCGCGTCTGTGCTGACTGCACTGGAAACCGCTCTGAACGGTGAAGACAAAGCCGCTATCGAAGCGAACATGCCGGACCTGGCACAGGTTCCTCAGAAACTGATGGAAATCGCCCAGCAGCAACATGccc                             >  2:859417/1‑151 (MQ=255)                                                                                                                    cgacAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCcagcagc                       >  1:526424/1‑149 (MQ=255)                                                                                                                    cgacAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCcagcagc                       >  1:332085/1‑149 (MQ=255)                                                                                                                      acAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGAc                   >  1:44247/1‑151 (MQ=255)                                                                                                                       cAAAACTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACt                  <  1:266570/151‑1 (MQ=255)                                                                                                                        aaaaCTGCTATCGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTg                 >  1:96513/1‑151 (MQ=255)                                                                                                                                   cGAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGAt      >  2:63299/1‑151 (MQ=255)                                                                                                                                    gAGTCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATg     >  1:304063/1‑151 (MQ=255)                                                                                                                                       tCTGTGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCtt  >  1:39624/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                   ACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTT  >  NC_000913/13727‑14011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑