Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,711,501	A→G	N225S (AAC→AGC)	rsxE →	SoxR iron‑sulfur cluster reduction factor component; electron transport inner membrane NADH‑quinone reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,711,501	0	A	G	100.0%	182.3 / NA	52	N225S (AAC→AGC)	rsxE	SoxR iron‑sulfur cluster reduction factor component; electron transport inner membrane NADH‑quinone reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

ATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGG  >  NC_000913/1711353‑1711646                                                                                                                                                     |                                                                                                                                                  aTTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCg                                                                                                                                                 <  2:897794/151‑1 (MQ=255)   tttCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGGCTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGt                                                                                                                                               >  2:400547/1‑151 (MQ=255)   tttCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCgg                                                                                                                                                >  1:139951/1‑150 (MQ=255)    ttCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGt                                                                                                                                               <  2:742130/150‑1 (MQ=255)         cacCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAAc                                                                                                                                          >  1:798557/1‑150 (MQ=255)          acCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAg                                                                                                                                        <  1:539072/151‑1 (MQ=255)           ccGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGATGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAgg                                                                                                                                       >  1:811415/1‑151 (MQ=255)             gACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGa                                                                                                                                     <  2:381791/151‑1 (MQ=255)              aCTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGaa                                                                                                                                    <  2:356611/151‑1 (MQ=255)               cTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAAt                                                                                                                                   >  1:676352/1‑151 (MQ=255)                tCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATg                                                                                                                                  >  2:373272/1‑151 (MQ=255)                 ccccTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAAt                                                                                                                                   <  1:423982/149‑1 (MQ=255)                  cccTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTc                                                                                                                                >  2:743025/1‑151 (MQ=255)                                cGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCa                                                                                                                  <  2:168518/151‑1 (MQ=255)                                 gATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCa                                                                                                                  >  2:287297/1‑150 (MQ=255)                                   tgctgcCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCaaa                                                                                                                >  1:108081/1‑150 (MQ=255)                                      tgcCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCTGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACg                                                                                                             <  1:586494/150‑1 (MQ=255)                                                       aTTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTc                                                                                            <  2:350542/150‑1 (MQ=255)                                                       aTTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCg                                                                                           >  1:369882/1‑151 (MQ=255)                                                           gCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTg                                                                                       >  1:826770/1‑151 (MQ=255)                                                              tGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCg                                                                                     >  1:377547/1‑150 (MQ=255)                                                              tGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGt                                                                                    >  2:881660/1‑151 (MQ=255)                                                                    tGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAAc                                                                              >  1:710605/1‑151 (MQ=255)                                                                     gATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGaa                                                                               <  2:345275/149‑1 (MQ=255)                                                                       tGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAAt                                                                           >  1:164891/1‑151 (MQ=255)                                                                           ggCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCt                                                                        <  2:626242/150‑1 (MQ=255)                                                                               ggAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATcc                                                                   <  2:734267/151‑1 (MQ=255)                                                                                 acaaTACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATccc                                                                  >  2:172848/3‑150 (MQ=255)                                                                                          gattgatGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAg                                                         <  1:60662/150‑1 (MQ=255)                                                                                           attgatGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCt                                                       >  1:650507/1‑151 (MQ=255)                                                                                                tgaAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTTATCCCACCACCGAGCTTAAtt                                                  >  2:70624/1‑151 (MQ=255)                                                                                                 gaAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAAtt                                                  <  2:430869/150‑1 (MQ=255)                                                                                                  aaaGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTc                                                >  2:297889/1‑151 (MQ=255)                                                                                                  aaaGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTc                                                <  1:63704/151‑1 (MQ=255)                                                                                                   aaGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTc                                                >  2:430898/1‑150 (MQ=255)                                                                                                   aaGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCa                                               <  1:285031/151‑1 (MQ=255)                                                                                                     gAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGt                                             <  2:51568/151‑1 (MQ=255)                                                                                                      aaTGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGtt                                            <  2:798102/151‑1 (MQ=255)                                                                                                         gAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCGCACCACCGAGCTTAATTTCAGTTCGc                                         >  2:294945/1‑151 (MQ=255)                                                                                                         gAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGc                                         >  2:85945/1‑151 (MQ=255)                                                                                                            aaaGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCtt                                      >  1:24835/1‑151 (MQ=255)                                                                                                                cgcCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg                                   >  1:342876/1‑150 (MQ=255)                                                                                                                cgcCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGa                                  <  2:790149/151‑1 (MQ=255)                                                                                                                 gcCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGaa                                 <  2:574957/151‑1 (MQ=255)                                                                                                                      gCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt                            <  2:220574/151‑1 (MQ=255)                                                                                                                              agcTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCg                    <  1:743075/151‑1 (MQ=255)                                                                                                                               gcTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCg                    <  2:432738/150‑1 (MQ=255)                                                                                                                                      aaCGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCtt            >  2:20317/1‑151 (MQ=255)                                                                                                                                       aCGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCttt           <  2:539863/151‑1 (MQ=255)                                                                                                                                        cGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTc          >  2:596719/1‑151 (MQ=255)                                                                                                                                         gTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTcc         >  1:380624/1‑151 (MQ=255)                                                                                                                                                gCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAgg  <  1:679143/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGG  >  NC_000913/1711353‑1711646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑