Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,240	T→C	E162G (GAA→GGA)	ydhW ←	FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,240	0	T	C	100.0%	204.1 / NA	57	E162G (GAA→GGA)	ydhW	FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/25);  total (32/25)

CTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCCGCGT  >  NC_000913/1751101‑1751387                                                                                                                                            |                                                                                                                                                    cTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCt                                                                                                                                          >  2:600673/1‑151 (MQ=255)   cGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCt                                                                                                                                          <  1:862576/149‑1 (MQ=255)    gAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTcc                                                                                                                                       >  1:202461/1‑151 (MQ=255)    gAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTcc                                                                                                                                       >  1:595502/1‑151 (MQ=255)       ccATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTg                                                                                                                                     <  1:448287/150‑1 (MQ=255)         aTTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGa                                                                                                                                   <  1:725940/150‑1 (MQ=255)         aTTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGa                                                                                                                                   >  1:516452/1‑150 (MQ=255)          ttCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGaa                                                                                                                                  <  2:183001/150‑1 (MQ=255)            cGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGt                                                                                                                                >  1:657249/1‑150 (MQ=255)                  agccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATgg                                                                                                                         >  1:429575/1‑151 (MQ=255)                        aagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTg                                                                                                                   >  2:347973/1‑151 (MQ=255)                           ccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCAt                                                                                                                >  2:300999/1‑151 (MQ=255)                                ccATAGGTCATAAAACGCTCGCTAAACAAGTACAACCCCGCCGTCAATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAg                                                                                                             <  1:704230/149‑1 (MQ=255)                                  aaaggcaaaaaaTCTCTCGCTAAACAAGAACAACACCGCCGTCGATGCCTCCGCCTGCCGTATATCGGCATATTCAGGTGCAACATCCCTCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAAGATGGCGCTTGCATCAGCAt                                                                                                          <  2:201106/141‑1 (MQ=255)                                  aTAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATg                                                                                                         <  1:127254/151‑1 (MQ=255)                                  aTAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATg                                                                                                         >  1:812691/1‑151 (MQ=255)                                           aaaTCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTg                                                                                                <  1:851313/151‑1 (MQ=255)                                            aaTCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGGATATCGGCATATTCAGGAGCAACATCCATCGCGGCGATCGCGTCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGt                                                                                               >  1:378540/1‑151 (MQ=255)                                                 ctcgctAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAgg                                                                                           <  1:76411/150‑1 (MQ=255)                                                   cgctAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGa                                                                                          <  1:168750/149‑1 (MQ=255)                                                        aaCAAGTACAACACCGCCGTCGATGACTCCACCTGCAGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTACTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGAt                                                                                   >  2:292920/1‑151 (MQ=255)                                                                 acccccgccgTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTg                                                                            <  2:3711/145‑1 (MQ=255)                                                                       gccgTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCa                                                                     >  1:673440/1‑150 (MQ=255)                                                                       gccgTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCa                                                                     >  2:831917/1‑150 (MQ=255)                                                                         cgTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCaaa                                                                   >  1:320884/1‑150 (MQ=255)                                                                            cGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacg                                                                >  2:670928/1‑150 (MQ=255)                                                                                 aCTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGc                                                          >  1:557992/1‑151 (MQ=255)                                                                                   tCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCg                                                         >  2:529158/1‑150 (MQ=255)                                                                                      aCCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGtgtg                                                     >  1:635912/1‑151 (MQ=255)                                                                                       ccTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGtgtg                                                     <  2:466660/150‑1 (MQ=255)                                                                                        cTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGc                                                    >  1:853230/1‑150 (MQ=255)                                                                                          gCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAAt                                                 >  2:396272/1‑151 (MQ=255)                                                                                            cGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATgg                                               <  2:557942/151‑1 (MQ=255)                                                                                                 atCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGcgcg                                           >  2:854595/1‑150 (MQ=255)                                                                                                   cGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGAc                                         >  2:227343/1‑150 (MQ=255)                                                                                                    ggCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACa                                        >  2:214809/1‑150 (MQ=255)                                                                                                      cATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAAt                                     <  2:105736/151‑1 (MQ=255)                                                                                                             aGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGc                              <  1:626477/151‑1 (MQ=255)                                                                                                                   aaCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAAt                                                 <  1:549867/126‑1 (MQ=255)                                                                                                                   aaCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAAt                                                 >  2:549817/1‑126 (MQ=255)                                                                                                                     catccatcGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCTCaa                       >  2:727663/1‑150 (MQ=255)                                                                                                                     catccatcGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAAc                      <  2:661454/151‑1 (MQ=255)                                                                                                                         catcGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGc                  >  2:203180/1‑151 (MQ=255)                                                                                                                          atcGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGc                  >  1:639907/1‑150 (MQ=255)                                                                                                                            cgcgGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCaaa               <  2:154114/151‑1 (MQ=255)                                                                                                                            cgcgGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCaa                <  2:312889/150‑1 (MQ=255)                                                                                                                            cgcgGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCaa                <  2:56646/150‑1 (MQ=255)                                                                                                                               ggcgATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGAc            >  1:240548/1‑151 (MQ=255)                                                                                                                                  gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc         <  2:331706/151‑1 (MQ=255)                                                                                                                                  gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc         >  1:580103/1‑151 (MQ=255)                                                                                                                                  gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc         <  2:52482/151‑1 (MQ=255)                                                                                                                                  gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc         >  1:856987/1‑151 (MQ=255)                                                                                                                                   aTCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATcg        <  1:302755/151‑1 (MQ=255)                                                                                                                                     cgcgGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATcgcc      <  1:361144/151‑1 (MQ=255)                                                                                                                                        ggCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCcgcg   <  2:467595/151‑1 (MQ=255)                                                                                                                                        ggCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCcgcg   >  2:342354/1‑151 (MQ=255)                                                                                                                                         gCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCCGCGt  >  2:890654/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                    CTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCCGCGT  >  NC_000913/1751101‑1751387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑