Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,475	T→C	Y257C (TAC→TGC)	ydiU ←	UPF0061 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,475	0	T	C	100.0%	177.5 / NA	50	Y257C (TAC→TGC)	ydiU	UPF0061 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/20);  total (30/20)

ACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCA  >  NC_000913/1790331‑1790621                                                                                                                                                 |                                                                                                                                                   acaaATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCa                                                                                                                                               >  1:426890/1‑150 (MQ=255)   aaaTGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGc                                                                                                                                            >  2:167811/1‑151 (MQ=255)       ggCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCa                                                                                                                                        <  1:43256/151‑1 (MQ=255)         cGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGc                                                                                                                                      >  2:845892/1‑151 (MQ=255)         cGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGc                                                                                                                                      >  1:524929/1‑151 (MQ=255)          gACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGcc                                                                                                                                     <  2:223169/151‑1 (MQ=255)              aTGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCcag                                                                                                                                 <  1:580198/151‑1 (MQ=255)                  cTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCcagc                                                                                                                                >  2:754245/1‑148 (MQ=255)                    gcgcCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCcagcag                                                                                                                              <  1:754295/148‑1 (MQ=255)                         aGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACAtgt                                                                                                                      <  2:753088/151‑1 (MQ=255)                            cGCTGTAAATTCCACAACGAGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGACAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACAtgttg                                                                                                                    >  1:757126/1‑150 (MQ=255)                             gCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTc                                                                                                                  >  2:669757/1‑151 (MQ=255)                                gTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCgg                                                                                                                <  2:808180/150‑1 (MQ=255)                                    aTTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATtc                                                                                                           <  2:42304/151‑1 (MQ=255)                                      tCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATtca                                                                                                          <  1:130769/150‑1 (MQ=255)                                                 aCGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATg                                                                                                >  2:149561/1‑149 (MQ=255)                                                  cGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGc                                                                                             <  2:265786/151‑1 (MQ=255)                                                      aGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCaa                                                                                           >  2:806404/1‑149 (MQ=255)                                                      aGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCaa                                                                                           >  2:298144/1‑149 (MQ=255)                                                          tGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCg                                                                                      <  1:603897/150‑1 (MQ=255)                                                           gATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGa                                                                                     <  2:753045/150‑1 (MQ=255)                                                                 cAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTg                                                                              <  2:1462/151‑1 (MQ=255)                                                                  aaaGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGc                                                                             >  2:269210/1‑151 (MQ=255)                                                                        gTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTgg                                                                       <  1:808921/151‑1 (MQ=255)                                                                             gCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAt                                                                  >  1:706421/1‑151 (MQ=255)                                                                              cccTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAtt                                                                 <  2:46692/151‑1 (MQ=255)                                                                                 ttGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAac                                                              >  1:785005/1‑151 (MQ=255)                                                                                  tgatgaTCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCCGCGACATGTTGTCGTTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATGAac                                                              <  2:357044/150‑1 (MQ=255)                                                                                         ccGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGt                                                      <  2:860790/151‑1 (MQ=255)                                                                                                 ttACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGc                                                >  1:29415/1‑149 (MQ=255)                                                                                                               gggTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAATCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGGCTGCCATTGGGCAATTAACAACGCGGTACGTGCGACAACATCGCTAAAc                                <  2:541317/151‑1 (MQ=255)                                                                                                               gggTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAc                                <  2:280528/151‑1 (MQ=255)                                                                                                                ggTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACACGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAcc                               >  2:554054/1‑151 (MQ=255)                                                                                                                 gTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAcc                               >  2:20282/1‑150 (MQ=255)                                                                                                                  ttCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAcc                               >  1:779245/1‑149 (MQ=255)                                                                                                                    cGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCag                             >  1:751288/1‑149 (MQ=255)                                                                                                                    cGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCag                             >  1:635372/1‑149 (MQ=255)                                                                                                                    cGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCag                             >  1:447791/1‑149 (MQ=255)                                                                                                                     gTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCag                             >  2:463835/1‑148 (MQ=255)                                                                                                                         tcatcaAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGt                      >  2:768364/1‑151 (MQ=255)                                                                                                                           atcaAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAt                    >  1:181175/1‑151 (MQ=255)                                                                                                                           atcaAGAAAACCAAACGGCCCGCAATCAAGAGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTa                     >  1:668199/1‑150 (MQ=255)                                                                                                                             caAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGCCGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAtt                   >  2:809614/1‑150 (MQ=255)                                                                                                                             caAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt                  >  1:525816/1‑151 (MQ=255)                                                                                                                              aaGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTg                 >  1:413719/1‑151 (MQ=255)                                                                                                                                      cAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc         >  1:63452/1‑151 (MQ=255)                                                                                                                                         aCGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCatcatc      >  1:601951/1‑151 (MQ=255)                                                                                                                                          cGGCCTGCAATCAAGCCTCAGCCCCAGCAACGACATGTTGTCGGTATTCATCACCCCATGAGCAAACCCGTCCGCCTGCCATTGGGCAATTAACGACGCGGTTCGTGCGACAACATCGCTAAACCAGGGACGGTATTTGTCCTCatcatc      <  2:248246/150‑1 (MQ=255)                                                                                                                                          cGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCt     <  2:418173/151‑1 (MQ=255)                                                                                                                                              ccGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCa  >  2:42513/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                   ACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCA  >  NC_000913/1790331‑1790621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑