Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,821,936	A→T	D107E (GAT→GAA)	osmE ←	osmotically‑inducible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,821,936	0	A	T	100.0%	238.0 / NA	63	D107E (GAT→GAA)	osmE	osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (43/20);  total (43/20)

TGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTC  >  NC_000913/1821791‑1822080                                                                                                                                                  |                                                                                                                                                 tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAg                                                                                                                                              <  2:831203/150‑1 (MQ=255)    ccTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTc                                                                                                                                          >  2:196041/1‑151 (MQ=255)      tgtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGt                                                                                                                                        >  1:646112/1‑151 (MQ=255)      tgtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGt                                                                                                                                        >  1:180272/1‑151 (MQ=255)         ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTAtt                                                                                                                                     >  1:645514/1‑151 (MQ=255)               gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGcac                                                                                                                               <  1:514896/151‑1 (MQ=255)                   ctctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGt                                                                                                                           >  2:149892/1‑151 (MQ=255)                       tCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTg                                                                                                                        <  2:329516/150‑1 (MQ=255)                       tCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTg                                                                                                                        >  1:604530/1‑150 (MQ=255)                         acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGAt                                                                                                                      >  1:236841/1‑150 (MQ=255)                            tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAAcc                                                                                                                  >  1:359689/1‑151 (MQ=255)                            tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAAcc                                                                                                                  >  1:903175/1‑151 (MQ=255)                               cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCgg                                                                                                                <  1:517737/150‑1 (MQ=255)                                    aaaTAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtga                                                                                                          <  2:599255/151‑1 (MQ=255)                                     aataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgat                                                                                                         <  2:43121/151‑1 (MQ=255)                                        aatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                                                                                                      >  1:861096/1‑151 (MQ=255)                                         atTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACa                                                                                                     <  2:559393/151‑1 (MQ=255)                                          ttCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCTGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACa                                                                                                     <  2:421301/150‑1 (MQ=255)                                             gcgAAGATAATTAAGAACTCAGGCCAAAAAAAACCAGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc                                                                                                 >  2:152081/1‑151 (MQ=255)                                              cgAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTcc                                                                                                >  2:544282/1‑151 (MQ=255)                                                  gATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGt                                                                                             >  1:118867/1‑150 (MQ=255)                                                    tAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatc                                                                                          >  2:179440/1‑151 (MQ=255)                                                        tAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcatc                                                                                       <  2:630983/150‑1 (MQ=255)                                                         aaGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCt                                                                                      >  1:654484/1‑150 (MQ=255)                                                         aaGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCt                                                                                      >  2:264038/1‑150 (MQ=255)                                                         aaGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCt                                                                                      >  1:765563/1‑150 (MQ=255)                                                           gAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAc                                                                                   >  2:728830/1‑151 (MQ=255)                                                           gAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAc                                                                                   >  2:361736/1‑151 (MQ=255)                                                            aaCTCAGGCCAATAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACGTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAATCGGAGTTGATGACATGTCCGGTATCATCTAAc                                                                                   <  2:647016/150‑1 (MQ=255)                                                            aaCTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAcg                                                                                  >  1:475055/1‑151 (MQ=255)                                                              cTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAcgcg                                                                                >  1:109542/1‑151 (MQ=255)                                                               tCAGGCTAAAAAAAACCGGCGCAATGGCCGGTTTCCGTGGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAcgcg                                                                                >  2:152135/1‑150 (MQ=255)                                                               tCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAcgcg                                                                                >  2:299682/1‑150 (MQ=255)                                                                cAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGa                                                                               <  1:813490/150‑1 (MQ=255)                                                                   gCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACaaa                                                                           <  1:429388/151‑1 (MQ=255)                                                                    ccAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAg                                                                          <  2:763584/151‑1 (MQ=255)                                                                             aCCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCt                                                                 <  2:155697/151‑1 (MQ=255)                                                                                    cAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTAc                                                          >  1:160997/1‑151 (MQ=255)                                                                                        ggCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATc                                                      >  1:362940/1‑151 (MQ=255)                                                                                             gTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGt                                                  >  1:720824/1‑150 (MQ=255)                                                                                                     tgttACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAgg                                         >  1:617661/1‑151 (MQ=255)                                                                                                      gttACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAgg                                         >  1:767858/1‑150 (MQ=255)                                                                                                      gttACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGa                                        <  2:16034/151‑1 (MQ=255)                                                                                                        tACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATg                                      >  1:318778/1‑151 (MQ=255)                                                                                                         aCTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGt                                     >  2:847556/1‑151 (MQ=255)                                                                                                            cAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAgg                                  >  2:442274/1‑151 (MQ=255)                                                                                                            cAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAgg                                  >  1:571618/1‑151 (MQ=255)                                                                                                               gCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCt                               >  1:796109/1‑151 (MQ=255)                                                                                                               gCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCt                               <  2:744367/151‑1 (MQ=255)                                                                                                                 tttCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTgg                             >  2:74276/1‑151 (MQ=255)                                                                                                                   tCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGc                            >  2:53169/1‑150 (MQ=255)                                                                                                                    cAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCa                           >  1:284921/1‑150 (MQ=255)                                                                                                                     aGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAg                         <  2:733312/151‑1 (MQ=255)                                                                                                                         gAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTAcc                     <  2:825845/151‑1 (MQ=255)                                                                                                                           aTTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACcgc                   >  1:367648/1‑151 (MQ=255)                                                                                                                               aTTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGa                 <  2:152254/149‑1 (MQ=255)                                                                                                                                ttACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGc               >  1:352628/1‑150 (MQ=255)                                                                                                                                     tCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATgg           <  2:693972/149‑1 (MQ=255)                                                                                                                                     tCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt         >  2:248939/1‑151 (MQ=255)                                                                                                                                      cGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATc        >  2:862567/1‑151 (MQ=255)                                                                                                                                        cAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCAt      >  1:196154/1‑151 (MQ=255)                                                                                                                                            cTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc  >  1:807665/1‑151 (MQ=255)                                                                                                                                             tgtgGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc  >  2:180801/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTC  >  NC_000913/1821791‑1822080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑