Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,859,148	A→G	F361S (TTC→TCC)	ydjK ←	putative MFS sugar transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,859,148	0	A	G	100.0%	173.5 / NA	46	F361S (TTC→TCC)	ydjK	putative MFS sugar transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

CAACGGCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGAAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGGC  >  NC_000913/1859002‑1859294                                                                                                                                                   |                                                                                                                                                   cAACGGCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGggaga                                                                                                                                                 >  2:192274/1‑150 (MQ=255)    cGGCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAAcc                                                                                                                                             >  1:109445/1‑151 (MQ=255)     ggCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCa                                                                                                                                            <  2:856504/151‑1 (MQ=255)        aTAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATc                                                                                                                                         >  2:873725/1‑151 (MQ=255)               gCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTa                                                                                                                                  <  1:234111/151‑1 (MQ=255)                         cACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACAt                                                                                                                        <  1:664037/151‑1 (MQ=255)                                tCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAAACAATCAACGTTATGAGCAACATACTGGt                                                                                                                  <  2:860642/150‑1 (MQ=255)                                 cGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGtt                                                                                                                 <  1:367714/150‑1 (MQ=255)                                    ccTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGc                                                                                                              >  1:339073/1‑150 (MQ=255)                                        ccGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTg                                                                                                          >  2:466104/1‑150 (MQ=255)                                              tCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGCAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAAt                                                                                                       >  2:520406/1‑147 (MQ=255)                                              tCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAAt                                                                                                       >  2:473220/1‑147 (MQ=255)                                               cGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGata                                                                                                  <  1:159339/151‑1 (MQ=255)                                                      ccGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGa                                                                                           <  1:330388/151‑1 (MQ=255)                                                        ggAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGc                                                                                         >  2:842145/1‑151 (MQ=255)                                                           gCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCAc                                                                                       <  1:678712/150‑1 (MQ=255)                                                               cGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCa                                                                                   >  1:269537/1‑150 (MQ=255)                                                                ggAGTTTGGCCTCCGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAAt                                                                                 <  1:623843/151‑1 (MQ=255)                                                                  aGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCa                                                                               <  1:368557/151‑1 (MQ=255)                                                                                 ggCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCGACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGcccc                                                                 >  1:478930/1‑150 (MQ=255)                                                                                  gCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCcac                                                               <  2:107601/151‑1 (MQ=255)                                                                                   ccAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCcac                                                               >  2:603323/1‑150 (MQ=255)                                                                                       aTTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCa                                                          <  2:903703/151‑1 (MQ=255)                                                                                       aTTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCa                                                          <  1:86736/151‑1 (MQ=255)                                                                                        tttCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCAt                                                         >  2:598937/1‑151 (MQ=255)                                                                                         ttCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCAt                                                         <  1:334661/150‑1 (MQ=255)                                                                                               gACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGtttt                                                   >  1:304259/1‑150 (MQ=255)                                                                                                aCATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTAc                                                 <  1:118032/151‑1 (MQ=255)                                                                                                    acacTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCgg                                             <  2:131400/151‑1 (MQ=255)                                                                                                    acacTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCgg                                             <  1:620821/151‑1 (MQ=255)                                                                                                        tGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAAtt                                         >  1:649796/1‑151 (MQ=255)                                                                                                                cATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCAt                                 >  1:513018/1‑151 (MQ=255)                                                                                                                cATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCAt                                 >  1:231301/1‑151 (MQ=255)                                                                                                                  tAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATc                                >  1:580284/1‑150 (MQ=255)                                                                                                                   aGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCa                               <  1:922/150‑1 (MQ=255)                                                                                                                      aaaCGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAg                           <  2:903870/151‑1 (MQ=255)                                                                                                                       aaCGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGc                          >  2:453169/1‑151 (MQ=255)                                                                                                                         cGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCAt                        >  2:356818/1‑151 (MQ=255)                                                                                                                              atatAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCa                    >  2:380669/1‑150 (MQ=255)                                                                                                                              atatAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCa                    >  1:484960/1‑150 (MQ=255)                                                                                                                                  aGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATaa                <  2:269511/150‑1 (MQ=255)                                                                                                                                  aGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATaa                >  1:726517/1‑150 (MQ=255)                                                                                                                                     cGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAAt            <  2:787922/151‑1 (MQ=255)                                                                                                                                      gAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATa           <  1:824698/151‑1 (MQ=255)                                                                                                                                         aGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCa        <  1:689681/151‑1 (MQ=255)                                                                                                                                            aaTCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAAt     <  2:663272/151‑1 (MQ=255)                                                                                                                                               cAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGgc  <  1:749158/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAACGGCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGAAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGGC  >  NC_000913/1859002‑1859294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑