Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,882	A→G	P197P (CCT→CCC)	mipA ←	scaffolding protein for murein synthesizing machinery

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,882	0	A	G	100.0%	185.1 / NA	54	P197P (CCT→CCC)	mipA	scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/30);  total (24/30)

TAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTA  >  NC_000913/1865736‑1866028                                                                                                                                                   |                                                                                                                                                   tAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGc                                                                                                                                                >  1:32476/1‑151 (MQ=255)    gTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTcc                                                                                                                                             >  1:908311/1‑151 (MQ=255)         cccGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTg                                                                                                                                        <  2:63447/151‑1 (MQ=255)                gAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCg                                                                                                                                 <  2:523792/151‑1 (MQ=255)                 aaaTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCgg                                                                                                                                <  2:20512/151‑1 (MQ=255)                 aaaTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCgg                                                                                                                                >  1:547602/1‑151 (MQ=255)                  aaTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCggg                                                                                                                               <  2:92146/151‑1 (MQ=255)                     cAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGtt                                                                                                                             >  1:118021/1‑150 (MQ=255)                        gCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAg                                                                                                                         <  2:342602/151‑1 (MQ=255)                           aGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCa                                                                                                                      <  1:690305/151‑1 (MQ=255)                                   aTTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGAcc                                                                                                              <  1:51061/151‑1 (MQ=255)                                       aTCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTg                                                                                                          <  2:80311/151‑1 (MQ=255)                                       aTCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTg                                                                                                          >  1:793966/1‑151 (MQ=255)                                         ccaccaTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCg                                                                                                        >  2:184267/1‑151 (MQ=255)                                          caccaTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCg                                                                                                        >  2:634262/1‑150 (MQ=255)                                          caccaTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGa                                                                                                       <  1:892173/151‑1 (MQ=255)                                          caccaTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGa                                                                                                       <  2:452156/151‑1 (MQ=255)                                            ccaTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGc                                                                                                     >  2:330875/1‑151 (MQ=255)                                                   gCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGActct                                                                                              >  2:189186/1‑151 (MQ=255)                                                     tGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCttt                                                                                            >  2:523264/1‑151 (MQ=255)                                                      gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCttt                                                                                            <  2:117999/150‑1 (MQ=255)                                                      gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCtt                                                                                             <  2:69916/149‑1 (MQ=255)                                                             aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATa                                                                                    <  2:109929/151‑1 (MQ=255)                                                                tcatcaGACAGGCGGGTGTAGCGTGCGGTGCCGTAATCACTCCTGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCACCAGGTAGGGGCTCCAGCTGTCGTTCGTGTTATAGCCGCGCAGACCGCTGCGTGCGGACTCTTTGCGCTATACg                                                                                  <  2:837799/150‑1 (MQ=255)                                                                    cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCata                                                                             <  2:463965/151‑1 (MQ=255)                                                                    cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCat                                                                              <  2:481363/150‑1 (MQ=255)                                                                      gacagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCataat                                                                           <  2:884341/151‑1 (MQ=255)                                                                           acGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTAt                                                                      <  1:867600/151‑1 (MQ=255)                                                                             gggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTc                                                                    >  2:260773/1‑151 (MQ=255)                                                                                tgtAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGtt                                                                 >  1:335370/1‑151 (MQ=255)                                                                                   aGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTg                                                              >  1:112144/1‑151 (MQ=255)                                                                                    gCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTg                                                              <  1:482447/150‑1 (MQ=255)                                                                                      gTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGtt                                                           <  1:848272/151‑1 (MQ=255)                                                                                         cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTc                                                        <  2:822961/151‑1 (MQ=255)                                                                                           gTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGc                                                      >  1:798071/1‑151 (MQ=255)                                                                                               cGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGt                                                   >  1:382735/1‑150 (MQ=255)                                                                                                  aaaCACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCa                                               <  2:725731/151‑1 (MQ=255)                                                                                                  aaaCACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTGCCa                                               <  2:291050/151‑1 (MQ=255)                                                                                                      acTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGTGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGc                                           >  2:408337/1‑151 (MQ=255)                                                                                                              cGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAt                                   >  1:769494/1‑151 (MQ=255)                                                                                                              cGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAt                                   >  1:234084/1‑151 (MQ=255)                                                                                                                    ggAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCg                              <  1:460652/150‑1 (MQ=255)                                                                                                                             aGCTGGCGCTCCGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCTACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGt                    <  1:792167/151‑1 (MQ=255)                                                                                                                             aGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACgg                     <  2:380762/150‑1 (MQ=255)                                                                                                                                 ggCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCa                  <  1:369620/149‑1 (MQ=255)                                                                                                                                 ggCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAgg                >  1:831913/1‑151 (MQ=255)                                                                                                                                    gcTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGCCAGGcc              >  1:499670/1‑150 (MQ=255)                                                                                                                                     cTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCAc            >  1:778500/1‑151 (MQ=255)                                                                                                                                       cAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCAc            <  2:32454/149‑1 (MQ=255)                                                                                                                                         gCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGtt        <  2:204731/151‑1 (MQ=255)                                                                                                                                          cTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTg       >  1:16651/1‑151 (MQ=255)                                                                                                                                           tCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTgg      >  2:349545/1‑151 (MQ=255)                                                                                                                                           tCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTgg      >  2:269517/1‑151 (MQ=255)                                                                                                                                               ggTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTa  <  1:351802/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTA  >  NC_000913/1865736‑1866028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑