Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,908,423	C→T	Q42Q (CAG→CAA)	yebO ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,908,423	0	C	T	100.0%	309.4 / NA	81	Q42Q (CAG→CAA)	yebO	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/34);  total (47/34)

CAGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATCTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGGC  >  NC_000913/1908276‑1908570                                                                                                                                                    |                                                                                                                                                    cAGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGt                                                                                                                                                  <  2:141656/151‑1 (MQ=255) cAGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGt                                                                                                                                                  >  1:330587/1‑151 (MQ=255)  aGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGtt                                                                                                                                                 <  2:818387/151‑1 (MQ=255)  aGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGtt                                                                                                                                                 >  2:760933/1‑151 (MQ=255)  aGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGtt                                                                                                                                                 <  2:508591/151‑1 (MQ=255)  aGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGtt                                                                                                                                                 >  1:354750/1‑151 (MQ=255)       aataatATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGtt                                                                                                                                             >  1:796362/1‑150 (MQ=255)          aataTCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTc                                                                                                                                         >  2:674996/1‑151 (MQ=255)          aataTCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTc                                                                                                                                         >  2:187961/1‑151 (MQ=255)              tCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTg                                                                                                                                     >  2:686232/1‑151 (MQ=255)                ttCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGa                                                                                                                                    >  2:772143/1‑150 (MQ=255)                 tCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGt                                                                                                                                      <  2:194993/147‑1 (MQ=255)                 tCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGaa                                                                                                                                   <  1:321148/150‑1 (MQ=255)                 tCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGa                                                                                                                                    <  1:765625/149‑1 (MQ=255)                       tCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGc                                                                                                                              <  1:109443/149‑1 (MQ=255)                       tCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCa                                                                                                                             <  2:19340/150‑1 (MQ=255)                          aCCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGAt                                                                                                                         <  2:211106/151‑1 (MQ=255)                          aCCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGAt                                                                                                                         >  2:249433/1‑151 (MQ=255)                             gATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTg                                                                                                                       >  1:489264/1‑150 (MQ=255)                               tttttGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGAt                                                                                                                     >  1:200286/1‑150 (MQ=255)                                 tttGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATaa                                                                                                                   <  2:201323/150‑1 (MQ=255)                                   tGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATaaaa                                                                                                                 <  1:474284/150‑1 (MQ=255)                                    gACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATaaaaa                                                                                                                >  1:793234/1‑150 (MQ=255)                                     aCTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAAc                                                                                                              <  2:269417/151‑1 (MQ=255)                                         tcAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACcac                                                                                                           >  1:555125/1‑150 (MQ=255)                                          cAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACcaca                                                                                                          >  1:82676/1‑150 (MQ=255)                                             cGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGAt                                                                                                      <  1:34011/151‑1 (MQ=255)                                                tCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGCCGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTCGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATg                                                                                                     >  2:746315/1‑149 (MQ=255)                                                tCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATg                                                                                                     >  2:236993/1‑149 (MQ=255)                                                tCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATg                                                                                                     >  2:272592/1‑149 (MQ=255)                                                 cTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGc                                                                                                  >  1:663769/1‑151 (MQ=255)                                                   ttttATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGccc                                                                                                >  1:720644/1‑151 (MQ=255)                                                    tttATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGccc                                                                                                <  1:186325/150‑1 (MQ=255)                                                       aTCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGAt                                                                                             >  1:313545/1‑150 (MQ=255)                                                         cTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATa                                                                                            >  1:169527/1‑149 (MQ=255)                                                             tttCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATaaga                                                                                         >  1:209355/1‑148 (MQ=255)                                                                        cGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCg                                                                            <  1:598757/150‑1 (MQ=255)                                                                          tttGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGAt                                                                          >  1:466448/1‑150 (MQ=255)                                                                           ttGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATAc                                                                        >  1:807475/1‑151 (MQ=255)                                                                            tGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATAc                                                                        >  1:642508/1‑150 (MQ=255)                                                                                tCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAAc                                                                     <  1:219399/149‑1 (MQ=255)                                                                                   cacaAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAg                                                                <  2:552289/151‑1 (MQ=255)                                                                                     caAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGAc                                                              <  1:237466/151‑1 (MQ=255)                                                                                      aaaCGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACg                                                             >  1:831244/1‑151 (MQ=255)                                                                                        acgacgTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCa                                                           <  1:856018/151‑1 (MQ=255)                                                                                        acgacgTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCa                                                           <  2:807410/151‑1 (MQ=255)                                                                                         cgacgTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCaa                                                          <  2:119592/151‑1 (MQ=255)                                                                                           acgTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATg                                                        <  2:800913/151‑1 (MQ=255)                                                                                           acgTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATg                                                        >  1:3721/1‑151 (MQ=255)                                                                                                aCAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTtcat                                                   <  1:101211/151‑1 (MQ=255)                                                                                                aCAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTtcat                                                   >  2:100939/1‑151 (MQ=255)                                                                                                aCAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTtcat                                                   >  1:214547/1‑151 (MQ=255)                                                                                                 cAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTtcatc                                                  >  2:225682/1‑151 (MQ=255)                                                                                                     gCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGc                                              >  1:720659/1‑151 (MQ=255)                                                                                                     gCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGc                                              <  1:141459/151‑1 (MQ=255)                                                                                                      cATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGcc                                             >  1:215576/1‑151 (MQ=255)                                                                                                          ttGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGa                                          >  1:738549/1‑150 (MQ=255)                                                                                                          ttGACGTGTTTGCTGATCCAGCAACGCCTCAAGCAGTTCTATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGTCCGATAAGAAGAACCGCCCCCGATACAACCAAAGACGCAATGTTCATCACGCCTGa                                          <  1:234944/150‑1 (MQ=255)                                                                                                           tGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAAt                                        >  2:253172/1‑151 (MQ=255)                                                                                                           tGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAAt                                        >  2:691978/1‑151 (MQ=255)                                                                                                             aCGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAAttt                                      >  2:465365/1‑151 (MQ=255)                                                                                                              cGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAAttt                                      >  2:549473/1‑150 (MQ=255)                                                                                                               gTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAAttt                                      <  2:67226/149‑1 (MQ=255)                                                                                                                  tttGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACaa                                  <  2:363443/150‑1 (MQ=255)                                                                                                                  tttGCTGATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAAc                                 >  2:743516/1‑151 (MQ=255)                                                                                                                        gATCCAGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCg                             <  2:20578/149‑1 (MQ=255)                                                                                                                             aGCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCAttt                      <  2:195439/151‑1 (MQ=255)                                                                                                                              gCAACGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTc                     <  1:566120/151‑1 (MQ=255)                                                                                                                                aaCGCCTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTcac                   >  1:332546/1‑151 (MQ=255)                                                                                                                                aaCGCCTCAAGCAGGTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTca                    >  2:321225/1‑150 (MQ=255)                                                                                                                                    ccTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCaccacc               <  1:451236/151‑1 (MQ=255)                                                                                                                                     cTCAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACct              >  1:23006/1‑151 (MQ=255)                                                                                                                                       cAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACctct            >  2:740897/1‑151 (MQ=255)                                                                                                                                       cAAGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACct              <  2:17966/149‑1 (MQ=255)                                                                                                                                        aaGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCTCGCCTGAATTTACAACTTCGTTCATTTCACCACCTCTg           <  2:77236/151‑1 (MQ=255)                                                                                                                                         aGCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACCTCTg           >  1:68705/1‑150 (MQ=255)                                                                                                                                          gCAGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAg         <  2:259304/151‑1 (MQ=255)                                                                                                                                            aGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATGCAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACctct            >  2:623807/1‑146 (MQ=255)                                                                                                                                            aGTTCAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAACATGGGCGCGATAGGAAGAACTACCCCCGACACAATCAAAGAGGCAATGTTCATCAAGCCTGAATTCACAAGTTCGTTCATTTCACAACCTCTGAg         >  2:602343/1‑149 (MQ=255)                                                                                                                                                cAATTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAgg   >  2:61486/1‑151 (MQ=255)                                                                                                                                                 aaTTTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGgc  >  2:325587/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGACGAATAATATCTTCGTCTTCAACCGATTTTTGACTCTCAACGGTCTTTTTATCTGCTTTCTCTGGTTCGTTTGCTTCGCACAAACGACGTAACAGTGCATTTTGACGTTTTTGCTGATCCAGCAACGCCTCAAGCAGTTCAATCTGTTCGTTAGTCCGTGAACTGGCACGATTGATAAAAAACCACAAGATGAGCCCGATAAGAAGAACCACCACCGATACAACCAAAGACGCAATGTTCATCACGCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGGC  >  NC_000913/1908276‑1908570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑