Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,009,257	T→C	G327G (GGT→GGC)	yedE →	UPF0394 family sulphur transport domain‑containing inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,009,257	0	T	C	100.0%	145.0 / NA	44	G327G (GGT→GGC)	yedE	UPF0394 family sulphur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/25);  total (19/25)

TAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGTCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCTGTTGTTTGCT  >  NC_000913/2009109‑2009404                                                                                                                                                     |                                                                                                                                                    tAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCt                                                                                                                                                   >  2:674953/1‑151 (MQ=255)  aGGCGTTGAACCCAAAATCATGTGGGCGTGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCt                                                                                                                                                   <  1:91974/150‑1 (MQ=255)  aGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCt                                                                                                                                                   <  2:832187/150‑1 (MQ=255)        tGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCaa                                                                                                                                             <  2:160083/150‑1 (MQ=255)        tGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAAt                                                                                                                                            >  2:290730/1‑151 (MQ=255)                       gtgGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAAc                                                                                                                              <  1:196871/150‑1 (MQ=255)                          ggCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGAtt                                                                                                                          >  2:711757/1‑151 (MQ=255)                             gggACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTg                                                                                                                       <  2:639287/151‑1 (MQ=255)                                    aaCGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTAtt                                                                                                                >  2:467923/1‑151 (MQ=255)                                       gcgGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACt                                                                                                             >  2:189433/1‑151 (MQ=255)                                       gcgGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACt                                                                                                             >  1:817706/1‑151 (MQ=255)                                       gcgGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACt                                                                                                             >  1:43375/1‑151 (MQ=255)                                        cgGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTg                                                                                                            >  1:748928/1‑151 (MQ=255)                                        cgGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTg                                                                                                            <  1:252507/151‑1 (MQ=255)                                         ggTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTgg                                                                                                           >  2:451060/1‑151 (MQ=255)                                          gTAATTGGTGGTTTACTGTTTGGTTTTGGCCGCGTGCTGGCTGGCGGCTGCGAATCCGGCTGGATGTAGCGCGCGGTAAAAGGCCAGGTGTACTACTGGTGTGTCGGCCTGGTCAATGTGATCGGCTCAACGATTCTGGCGTATTACTggg                                                                                                          <  2:338763/151‑1 (MQ=255)                                                 gtggtTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGAttt                                                                                                   >  1:397966/1‑151 (MQ=255)                                                            tttGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGgcgc                                                                                        >  1:324632/1‑151 (MQ=255)                                                                    tGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCAcc                                                                                >  1:170826/1‑151 (MQ=255)                                                                      gCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCTCTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCg                                                                               <  2:47802/150‑1 (MQ=255)                                                                       cATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGAc                                                                             >  2:310706/1‑151 (MQ=255)                                                                        aTCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACt                                                                            <  1:681656/151‑1 (MQ=255)                                                                          cGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTgg                                                                          <  1:347051/151‑1 (MQ=255)                                                                                  ctggcGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAAt                                                                  >  1:46592/1‑151 (MQ=255)                                                                                    ggcggcTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAAt                                                                  <  1:242099/149‑1 (MQ=255)                                                                                       ggcTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGAGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAAcc                                                             >  2:365706/1‑151 (MQ=255)                                                                                           gCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACctg                                                           <  2:421535/149‑1 (MQ=255)                                                                                                       ggATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTgg                                             <  1:567638/151‑1 (MQ=255)                                                                                                           gTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGCCTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCg                                         <  2:689258/151‑1 (MQ=255)                                                                                                            tACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCg                                         >  2:52510/1‑150 (MQ=255)                                                                                                             aCCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCAGGCGCTGGCCACCGACTGGGGCAAAAGCAACCTGCTGAAAACCTTTGGTcggc                                        <  2:502354/150‑4 (MQ=255)                                                                                                                 cgcgGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGt                                   <  1:850336/151‑1 (MQ=255)                                                                                                                   cgGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTgg                                 >  2:78399/1‑151 (MQ=255)                                                                                                                        gAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATgg                                     >  2:41751/1‑142 (MQ=255)                                                                                                                        gAAGGCCAGGTGCACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATgg                                     <  1:41773/142‑1 (MQ=255)                                                                                                                                    cACTACTGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATAtt                <  2:245980/151‑1 (MQ=255)                                                                                                                                      ctactGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTg              >  1:717325/1‑151 (MQ=255)                                                                                                                                        actGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTg              <  2:516255/149‑1 (MQ=255)                                                                                                                                        actGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTg              <  2:79040/149‑1 (MQ=255)                                                                                                                                         ctGGTGGGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCt            <  2:478244/150‑1 (MQ=255)                                                                                                                                           ggtggGTCGGCCTGTGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCtg           <  1:450355/149‑1 (MQ=255)                                                                                                                                           ggtggGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCtgt          <  1:38019/150‑1 (MQ=255)                                                                                                                                           ggtggGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCtgt          <  2:642147/150‑1 (MQ=255)                                                                                                                                            gtggGTCGGCCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCtgttg        <  1:498100/151‑1 (MQ=255)                                                                                                                                                 tcggccTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCTGTTGTTtgc   >  1:369311/1‑151 (MQ=255)                                                                                                                                                  cggccTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCTGTTGTTtgct  >  1:865852/1‑151 (MQ=255)                                                                                                                                                  cggccTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCTGTTGTTtgct  >  2:847463/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGTCTGGGCAATGTGATCGGCTCAACGATTCTGGCGTATTACTGGGATGATTTCGCTCCGGCGCTGGCCACCGACTGGGACAAAATCAACCTGCTGAAAACCTTTGGTCCGATGGGTGGCCTGCTGGTGACATATTTGCTGTTGTTTGCT  >  NC_000913/2009109‑2009404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑