Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,063,636	T→C	M278V (ATG→GTG)	cobT ←	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,063,636	0	T	C	100.0%	190.6 / NA	50	M278V (ATG→GTG)	cobT	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/27);  total (23/27)

CGCCAGAGCTGCGCCACTCCCCTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGG  >  NC_000913/2063493‑2063783                                                                                                                                                |                                                                                                                                                    cGCCAGAGCTGCGCCACTCCCCTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCa                                                                                                                                               <  2:367684/150‑1 (MQ=255)           gcgcCACTCCCCTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAgcg                                                                                                                                    <  2:102456/151‑1 (MQ=255)                   ccccTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCgcagcagc                                                                                                                             >  1:65308/1‑150 (MQ=255)                    cccTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCAt                                                                                                                           <  2:665582/151‑1 (MQ=255)                     ccTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCAt                                                                                                                           >  1:291223/1‑150 (MQ=255)                     ccTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCAt                                                                                                                           >  1:814684/1‑150 (MQ=255)                     ccTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATa                                                                                                                          <  2:777267/151‑1 (MQ=255)                     ccTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATa                                                                                                                          <  2:511584/151‑1 (MQ=255)                     ccTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATa                                                                                                                          <  2:146747/151‑1 (MQ=255)                             aaaCGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAagaaagaa                                                                                                                  <  1:368726/151‑1 (MQ=255)                              aaCGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAgaaagaaa                                                                                                                 <  2:826008/151‑1 (MQ=255)                                cGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAatc                                                                                                               <  1:473016/151‑1 (MQ=255)                                               agaTAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACgg                                                                                                >  2:517845/1‑151 (MQ=255)                                               agaTAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACg                                                                                                 <  2:259712/150‑1 (MQ=255)                                                gaTAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACggg                                                                                               <  2:42867/151‑1 (MQ=255)                                                          cAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGc                                                                                       >  1:358304/1‑149 (MQ=255)                                                                cAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAagca                                                                               <  1:69828/151‑1 (MQ=255)                                                                cAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAagca                                                                               <  2:107230/151‑1 (MQ=255)                                                                      cGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCt                                                                         >  1:390920/1‑151 (MQ=255)                                                                       gagaGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCt                                                                         >  2:95395/1‑150 (MQ=255)                                                                        agagCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTa                                                                        >  2:196000/1‑150 (MQ=255)                                                                          agCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACa                                                                     <  2:653836/151‑1 (MQ=255)                                                                            cgcTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATc                                                                   >  2:429127/1‑151 (MQ=255)                                                                                ataCGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTc                                                               <  1:289051/151‑1 (MQ=255)                                                                                     cgcgcCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCt                                                          <  2:713822/151‑1 (MQ=255)                                                                                      gcgcCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCt                                                          >  1:781765/1‑150 (MQ=255)                                                                                       cgcCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTAt                                                        >  1:830693/1‑151 (MQ=255)                                                                                             tttCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGAc                                                  >  2:755127/1‑151 (MQ=255)                                                                                               tCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGAcc                                                 >  2:52458/1‑150 (MQ=255)                                                                                                  ggtgACAGGTTAGAAGGAATGAGATACGTTTGGATCGGAGTAGACTCCTTCTACGTGGCGAGGGTAGCAGCATAAGTGAGAAATCCATCCAGCAACACGGGTAATCCGCAGGAAGAAGCACCGATCATCACTCCAGCTTTTCCGGCcagcc                                             <  2:516401/148‑4 (MQ=255)                                                                                                  gCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACcaaat                                             <  1:651257/151‑1 (MQ=255)                                                                                                   ccGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACcaaatc                                            <  1:117337/151‑1 (MQ=255)                                                                                                   ccGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACcaaatc                                            >  1:254823/1‑151 (MQ=255)                                                                                                          gTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATcc                                      >  2:71386/1‑150 (MQ=255)                                                                                                                aGGAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACt                               <  2:578860/151‑1 (MQ=255)                                                                                                                  gAATGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACttt                             <  2:565228/151‑1 (MQ=255)                                                                                                                    aTGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTg                           >  2:511581/1‑151 (MQ=255)                                                                                                                     tGAGATACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGc                          >  2:870892/1‑151 (MQ=255)                                                                                                                         aTACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAgg                      <  1:13488/151‑1 (MQ=255)                                                                                                                          tACGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAATTCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAgg                      >  2:477826/1‑150 (MQ=255)                                                                                                                            cGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGAc                    >  2:730345/1‑150 (MQ=255)                                                                                                                            cGGTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGAc                    >  1:36557/1‑150 (MQ=255)                                                                                                                             ggTTTGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACAt                  >  2:40244/1‑151 (MQ=255)                                                                                                                                ttGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCa                <  2:169635/150‑1 (MQ=255)                                                                                                                                 tGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCaa               <  2:188256/150‑1 (MQ=255)                                                                                                                                 tGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAac              >  1:540260/1‑151 (MQ=255)                                                                                                                                 tGATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAac              >  1:499380/1‑151 (MQ=255)                                                                                                                                  gATCGCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAaca             <  2:178134/151‑1 (MQ=255)                                                                                                                                      gCAGGAGACACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCAt         <  1:11334/151‑1 (MQ=255)                                                                                                                                          gagaCACCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTg     <  2:830653/151‑1 (MQ=255)                                                                                                                                             acacCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAgg  <  1:580973/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    CGCCAGAGCTGCGCCACTCCCCTCACCTAAACGCATCTCCATATTGAGATAAGGCTCCAGCCCCAAATGCGAGAGCGCTATACGCGCGCCTTTTTCTGCCGACAGGTGAGAAGGAATGAGATACGGTTTGATCGCAGGAGACATCTGGCAGGCTGCGAGCGCAGCAGCATAAGAAAGAAATCCATCCAGCAACACGGGTAAACCGCAGGAAGCAGCACCTAACATCACTCCAGCTATTCCGACCAAATCAAATCCACCCACTTTTGCCAGGACATCAACACCATCCTGAGG  >  NC_000913/2063493‑2063783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑