Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 308,755 C→T S193N (AGC→AAC)  ecpC ← ECP production outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913308,7550CT95.7% 167.6 / NA 47S193N (AGC→AAC) ecpCECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (34/11);  minor base G (0/2);  total (34/13)
Fisher's exact test for biased strand distribution p-value = 7.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTG  >  NC_000913/308609‑308898
                                                                                                                                                   |                                                                                                                                               
cGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTgg                                                                                                                                              >  1:396913/1‑150 (MQ=255)
    aaaTCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGAtgtg                                                                                                                                         <  2:73017/151‑1 (MQ=255)
      aTCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGtt                                                                                                                                       <  1:301691/151‑1 (MQ=255)
         ggTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCt                                                                                                                                    >  2:341574/1‑151 (MQ=255)
            gACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTccc                                                                                                                                 >  2:182661/1‑151 (MQ=255)
               cGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCccgcc                                                                                                                              >  1:813787/1‑151 (MQ=255)
                  cAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGtt                                                                                                                           >  1:405408/1‑151 (MQ=255)
                   aaaaTCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGtt                                                                                                                           >  2:175775/1‑150 (MQ=255)
                        cgcgTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCa                                                                                                                      >  1:695375/1‑150 (MQ=255)
                          cgTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACt                                                                                                                   >  2:38948/1‑151 (MQ=255)
                             tCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTTAGCGACAGATAGTTGGATGTGTTGCTACCGCCGTTACGCAAATGgt                                                                                                                >  2:685351/1‑151 (MQ=255)
                                 aCATCTTTTTATATAATTCCCTGTCCTGTTGCCCGCTACCGACCCCGTACAGCGGGCCGTCGAGCCCCACATGATGTCCGCGCAGTGCAGTAACTTTATTCAGCGACAGATGGTTGGTTGTTTTGCTCCCGCCGTTACGCAACTGgttgt                                                                                                             <  1:200522/150‑1 (MQ=255)
                                  cATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCTAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGgttgtt                                                                                                            >  2:348433/1‑150 (MQ=255)
                                     cGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTtat                                                                                                          >  2:675637/1‑149 (MQ=255)
                                       cTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGAc                                                                                                      >  1:511962/1‑151 (MQ=255)
                                          tatataATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGcc                                                                                                   >  1:97208/1‑151 (MQ=255)
                                          tatataATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGc                                                                                                    <  1:420788/150‑1 (MQ=255)
                                            tataATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGccc                                                                                                  >  1:850839/1‑150 (MQ=255)
                                              taATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCa                                                                                                 >  1:568802/1‑149 (MQ=255)
                                               aaTTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGt                                                                                              >  2:895290/1‑151 (MQ=255)
                                                   ccctctCCTGTTGCCCGCTACCGACCCCGTACGGCGGCCCGTCAGGCCCCACATGATGTTCGCGCAGTGCAGTACCTTTATTCAGCGACAAATAGTGGGATTTTTTCCTCCCGCCGTTACGCGACTGGTTGTTATAGACGCCCa                                                                                                 <  1:429093/139‑1 (MQ=255)
                                                   cACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTtat                                                                                           >  2:465057/1‑150 (MQ=255)
                                                           tGTTGACCCTTCCCGTCCCCGTACAGCGGGCCGCCGACCCCCATATGTTGTTCGCGCGTGGCAGTAACTTTTTTCGGCGACAATTTGTGGGTTTTTTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGa                                                                                   <  1:114105/150‑1 (MQ=255)
                                                              tccccGCTCCCGACCCCGTACAGCGAGCCGTCGAGCCCCACATGTTGTCCGCGCAGTGCAGTACCTTTATCCAGCGAAAGATAGTTGGTTGTTTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTa                                                                                <  2:58318/147‑1 (MQ=255)
                                                                            cccGTACAGCGCGCCGCCGCGCACCACATGGTGTGCGCGCTGTGCATTAACGTTATCCAGAGAGAGATAGGTGGGTGTGTCGCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGgtg                                                                    <  2:42995/148‑1 (MQ=255)
                                                                             cctaccggcggcccgccgACCACCAAATGTGTTCCGCGCGGTGCAGTAACTTTATTCAGCGAAAGATATTTGGTTGTGTTCCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGgtg                                                                    <  2:642171/135‑1 (MQ=255)
                                                                                 aCAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACt                                                            >  1:402336/1‑151 (MQ=255)
                                                                                      gggCCGGCGAGCACCACTTGTTGTTCGCGCGGTGAAGTAACTTTATTCAGCAAATGATAGTTGTTTTTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGAc                                                        <  2:611873/148‑1 (MQ=255)
                                                                                            tCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCg                                                  >  1:301324/1‑150 (MQ=255)
                                                                                               aGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGt                                              >  1:66009/1‑151 (MQ=255)
                                                                                                  accacATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCtt                                           >  2:592658/1‑151 (MQ=255)
                                                                                                   ccacATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTc                                          >  2:504718/1‑151 (MQ=255)
                                                                                                   ccacATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTc                                          >  2:734018/1‑151 (MQ=255)
                                                                                                       atgatgTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCt                                       >  1:859196/1‑150 (MQ=255)
                                                                                                            gTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTg                                  >  1:288127/1‑150 (MQ=255)
                                                                                                              tccgccCAGTCAAGTAACTTTTTTGAGCGAGAAATTGGTGGTGTTGTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTc                                             <  2:605375/136‑1 (MQ=255)
                                                                                                                      gtgaattAACGTTATCCAGCGAAAGATGGTTGTGTGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCgtag                           <  1:29691/142‑1 (MQ=255)
                                                                                                                        gcagtATCTTTATTCAGCAACATATGGTTGGTTGTTTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACg                       <  1:877779/150‑1 (MQ=255)
                                                                                                                          agtAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTg                    >  1:416056/1‑151 (MQ=255)
                                                                                                                          agtAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTg                    >  2:810230/1‑151 (MQ=255)
                                                                                                                               cGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGccc                 >  2:760773/1‑149 (MQ=255)
                                                                                                                                 ttATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAgcg             >  2:741148/1‑151 (MQ=255)
                                                                                                                                  tATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAgcgc            >  1:686278/1‑151 (MQ=255)
                                                                                                                                   aTTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCt           >  2:636881/1‑151 (MQ=255)
                                                                                                                                    ttCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCtt          >  2:578672/1‑151 (MQ=255)
                                                                                                                                     tCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTc         >  1:193628/1‑151 (MQ=255)
                                                                                                                                             aGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTg  >  2:713481/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTG  >  NC_000913/308609‑308898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: