Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,465	G→A	S28L (TCA→TTA)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,465	0	G	A	100.0%	174.4 / NA	46	S28L (TCA→TTA)	dacD	D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/22);  total (24/22)

AGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTGAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGA  >  NC_000913/2082320‑2082611                                                                                                                                                  |                                                                                                                                                   aGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTTGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTaaaaaa                                                                                                                                               <  2:642115/151‑1 (MQ=255)      gATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAAt                                                                                                                                          <  2:77393/151‑1 (MQ=255)         ccACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGtt                                                                                                                                       >  1:186419/1‑151 (MQ=255)              aCATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGAAGCTCATTACCCGCGGTGAGGATCTGACCGGTGGGGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTcc                                                                                                                                   >  2:130717/1‑150 (MQ=255)                   aCCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGGGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGGAAAAAAGGAATGTTTTccgcc                                                                                                                                >  2:225584/1‑148 (MQ=255)                         catcaGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCa                                                                                                                       <  1:274542/151‑1 (MQ=255)                             aGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACg                                                                                                                   <  1:596208/151‑1 (MQ=255)                                 ttGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATa                                                                                                                <  2:881158/150‑1 (MQ=255)                                     cAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGtt                                                                                                            <  1:105183/150‑1 (MQ=255)                                      aGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTaa                                                                                                          <  1:276761/151‑1 (MQ=255)                                        gCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTaaaa                                                                                                        <  2:158464/151‑1 (MQ=255)                                          tGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAAc                                                                                                      >  1:356799/1‑151 (MQ=255)                                           ggCGGGATTGCGCTGTTGATGCTCAGTACCCGCGGTGAGGATATGAACGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACg                                                                                                     >  2:648173/1‑151 (MQ=255)                                              gggATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGaa                                                                                                   <  2:23474/150‑1 (MQ=255)                                               ggATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACa                                                                                                 <  2:182234/151‑1 (MQ=255)                                                   tGCGCTGATGATGCTCATTACCCGCGGTGAGGATCTGTCCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAAACTCTGGAGGCTGAGGGAAAAAAGGAAATTTTTCCACCGCAAAACCAGACGATCAGTTAAATACGAACaa                                                                                                >  2:754807/1‑148 (MQ=255)                                                          ttGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAagcagc                                                                                       >  1:867466/1‑150 (MQ=255)                                                            gATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCaat                                                                                    <  2:109021/151‑1 (MQ=255)                                                                cTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCaataat                                                                                 >  2:909104/1‑150 (MQ=255)                                                                  cATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAAtaataa                                                                               <  2:69316/150‑1 (MQ=255)                                                                    ttACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGAc                                                                            >  2:396214/1‑151 (MQ=255)                                                                     tACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGAc                                                                            <  2:571994/150‑1 (MQ=255)                                                                     tACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACg                                                                           >  1:568843/1‑151 (MQ=255)                                                                         cgcgGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGt                                                                       >  1:43643/1‑151 (MQ=255)                                                                                  gATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGc                                                              >  2:409466/1‑151 (MQ=255)                                                                                               gtgtAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAg                                                 <  1:224044/151‑1 (MQ=255)                                                                                                    aTCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCa                                            <  1:374535/151‑1 (MQ=255)                                                                                                       cATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAAt                                         <  1:23402/151‑1 (MQ=255)                                                                                                              aCCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGtt                                  >  1:512261/1‑151 (MQ=255)                                                                                                              aCCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGtt                                  >  2:464235/1‑151 (MQ=255)                                                                                                               cccAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTc                                 >  1:645849/1‑151 (MQ=255)                                                                                                                   ggACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCtttt                             >  2:205021/1‑151 (MQ=255)                                                                                                                      cccGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTAc                          >  2:733983/1‑151 (MQ=255)                                                                                                                       ccGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACg                         <  1:625083/151‑1 (MQ=255)                                                                                                                       ccGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACg                         >  1:74469/1‑151 (MQ=255)                                                                                                                         ggCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACgg                        >  1:205656/1‑150 (MQ=255)                                                                                                                          gCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGa                       >  2:157133/1‑150 (MQ=255)                                                                                                                          gCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGa                       >  1:305100/1‑150 (MQ=255)                                                                                                                            aTGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAAt                    >  1:715367/1‑151 (MQ=255)                                                                                                                                   tctGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATg             >  1:622776/1‑151 (MQ=255)                                                                                                                                     tGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGa            >  2:756450/1‑150 (MQ=255)                                                                                                                                      ggAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGa            <  2:344532/149‑1 (MQ=255)                                                                                                                                          gCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAActggc      <  1:671288/151‑1 (MQ=255)                                                                                                                                             gAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAActggctg    <  2:693581/150‑1 (MQ=255)                                                                                                                                             gAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAActggctg    <  1:471207/150‑1 (MQ=255)                                                                                                                                             gAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAActggct     <  2:166745/149‑1 (MQ=255)                                                                                                                                              aGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGa  <  2:105438/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   AGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTGAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGA  >  NC_000913/2082320‑2082611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑