Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,750	T→C	H112R (CAC→CGC)	wbbJ ←	putative lipopolysaccharide biosynthesis O‑acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,750	0	T	C	100.0%	234.1 / NA	66	H112R (CAC→CGC)	wbbJ	putative lipopolysaccharide biosynthesis O‑acetyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/31);  total (35/31)

ACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGT  >  NC_000913/2104612‑2104893                                                                                                                                           |                                                                                                                                                aCGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAAc                                                                                                                                     <  1:618324/151‑1 (MQ=255)    aCTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGt                                                                                                                                  >  1:265277/1‑151 (MQ=255)       ccATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGa                                                                                                                                <  2:265251/150‑1 (MQ=255)       ccATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGAt                                                                                                                               <  1:653946/151‑1 (MQ=255)         aTTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGAtt                                                                                                                              >  1:376334/1‑150 (MQ=255)              cAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATc                                                                                                                        >  2:379728/1‑151 (MQ=255)              cAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATc                                                                                                                        >  1:519683/1‑151 (MQ=255)               aataatTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCg                                                                                                                       >  2:900523/1‑151 (MQ=255)               aataatTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCg                                                                                                                       >  2:17829/1‑151 (MQ=255)               aataatTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCg                                                                                                                       >  1:786070/1‑151 (MQ=255)               aataatTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCg                                                                                                                       >  1:750973/1‑151 (MQ=255)                  aatTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGt                                                                                                                     >  2:615477/1‑150 (MQ=255)                   atTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGta                                                                                                                    >  2:546309/1‑150 (MQ=255)                      gTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGtaata                                                                                                                 >  1:627563/1‑150 (MQ=255)                      gTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGtaata                                                                                                                 >  1:544685/1‑150 (MQ=255)                            ggCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAAtactt                                                                                                          <  1:39167/151‑1 (MQ=255)                              cAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAAtacttta                                                                                                        <  2:396821/151‑1 (MQ=255)                               aaaaCCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAAtactttac                                                                                                       <  1:257092/151‑1 (MQ=255)                                aaaCCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAAtactttact                                                                                                      <  2:662347/151‑1 (MQ=255)                                 aaCCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAAtactttactt                                                                                                     <  1:562565/151‑1 (MQ=255)                                   ccGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGc                                                                                                   <  1:227427/151‑1 (MQ=255)                                    cGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCa                                                                                                  <  2:740680/151‑1 (MQ=255)                                       cacaTTCTCACCCACCCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATa                                                                                               <  2:89614/151‑1 (MQ=255)                                           ttCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCg                                                                                           >  1:244654/1‑151 (MQ=255)                                                aCCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATcc                                                                                      >  1:5427/1‑151 (MQ=255)                                                 cccAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATccc                                                                                     >  1:382731/1‑151 (MQ=255)                                                     accaaACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGAcc                                                                                 <  2:827742/151‑1 (MQ=255)                                                      ccaaACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCt                                                                                >  1:20309/1‑151 (MQ=255)                                                       caaACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTa                                                                               <  2:552270/151‑1 (MQ=255)                                                         aaCCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATc                                                                             <  1:403560/151‑1 (MQ=255)                                                         aaCCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATc                                                                             >  2:111556/1‑151 (MQ=255)                                                          aCCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTCCGCATGTCTGGAGGTATATTTGGCCAACTCATTGGATCAGAGCGCTTAAAGTAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAAGAAGCGTATCCCGACCTACCg                                                                            <  2:167062/151‑1 (MQ=255)                                                           cccTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCg                                                                            <  1:232992/150‑1 (MQ=255)                                                           cccTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGt                                                                           <  1:244009/151‑1 (MQ=255)                                                            ccTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGt                                                                           >  1:324983/1‑150 (MQ=255)                                                             ctctGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTa                                                                          >  1:499205/1‑150 (MQ=255)                                                                 ggCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAAc                                                                        <  1:442892/148‑1 (MQ=255)                                                                   ccAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGctct                                                                   >  1:279372/1‑151 (MQ=255)                                                                    cAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCtctc                                                                  >  1:106912/1‑151 (MQ=255)                                                                     aaTTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCtctc                                                                  >  2:763756/1‑150 (MQ=255)                                                                             caGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGc                                                         >  2:166686/1‑151 (MQ=255)                                                                                   aaGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATg                                                   <  1:162549/151‑1 (MQ=255)                                                                                   aaGATTCCAACGTGCGCATGTCT‑GAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGa                                                  <  1:321354/151‑1 (MQ=255)                                                                                    aGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATg                                                   >  2:797913/1‑150 (MQ=255)                                                                                    aGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATg                                                   >  1:339961/1‑150 (MQ=255)                                                                                      aTTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGaa                                                 <  2:627514/150‑1 (MQ=255)                                                                                        tCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACa                                               <  2:668341/150‑1 (MQ=255)                                                                                        tCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACa                                               <  1:652254/150‑1 (MQ=255)                                                                                         ccAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACAt                                              <  2:422695/150‑1 (MQ=255)                                                                                             cGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCg                                         >  2:883066/1‑151 (MQ=255)                                                                                              gTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGt                                        <  1:546359/151‑1 (MQ=255)                                                                                                 cgcATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTa                                      >  2:74686/1‑150 (MQ=255)                                                                                                   cATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACt                                   >  1:500543/1‑151 (MQ=255)                                                                                                    aTGTCTGGAGGTATATTTGACGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAAGAAGCGTATCCCGACCTGTCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAAc                                    <  1:841919/149‑1 (MQ=255)                                                                                                      gTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGc                                >  1:666152/1‑151 (MQ=255)                                                                                                              gTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGGTTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATc                        <  1:344169/151‑1 (MQ=255)                                                                                                              gTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATc                        >  1:355271/1‑151 (MQ=255)                                                                                                                atatTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCgg                      >  1:403228/1‑151 (MQ=255)                                                                                                                   tttGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGaa                    >  2:319760/1‑150 (MQ=255)                                                                                                                    ttGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGaaa                   <  2:223112/150‑1 (MQ=255)                                                                                                                       gCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGaaaaaa                >  2:446513/1‑150 (MQ=255)                                                                                                                          aaCTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGACGTTAACTTGCACATTATCGGAAAAAAAAAt            <  1:845257/151‑1 (MQ=255)                                                                                                                              cATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACg        <  1:158771/151‑1 (MQ=255)                                                                                                                                 tGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGcc      >  1:650544/1‑150 (MQ=255)                                                                                                                                   gATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACg   <  1:338050/151‑1 (MQ=255)                                                                                                                                    aTCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGt  >  2:35509/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                ACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGT  >  NC_000913/2104612‑2104893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑