Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,182,120	A→G	V221A (GTG→GCG)	yegW ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,182,120	0	A	G	100.0%	198.9 / NA	56	V221A (GTG→GCG)	yegW	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/29);  total (27/29)

GGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGA  >  NC_000913/2181988‑2182259                                                                                                                                     |                                                                                                                                            gggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAg                                                                                                                             >  1:664969/1‑149 (MQ=255) gggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCa                                                                                                                           <  2:67388/151‑1 (MQ=255)   gTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCAcc                                                                                                                         <  1:48417/151‑1 (MQ=255)      gTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCgg                                                                                                                       <  2:782414/150‑1 (MQ=255)        cGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTa                                                                                                                     <  2:679802/150‑1 (MQ=255)        cGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTAt                                                                                                                    <  2:86816/151‑1 (MQ=255)          ggCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTAttt                                                                                                                  >  1:859877/1‑151 (MQ=255)                          ttGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGaa                                                                                                   <  2:542496/150‑1 (MQ=255)                            gtgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAatat                                                                                                >  1:902579/1‑151 (MQ=255)                            gtgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAatat                                                                                                <  1:688279/151‑1 (MQ=255)                             tgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATAtg                                                                                               >  1:558533/1‑151 (MQ=255)                                  cAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACt                                                                                          <  1:65785/151‑1 (MQ=255)                                    aCGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTg                                                                                         >  1:447553/1‑150 (MQ=255)                                    aCGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGa                                                                                        <  1:78411/151‑1 (MQ=255)                                     cgcgcgTGGACCTTCTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAAGAGGCCGTTGCTGTTGGTCAAGCACCGATTGCTTGAGCACCAGCACCGGTATGTTGCTATCTAACTGAAAATGTTACTg                                                                                         <  2:754174/149‑1 (MQ=255)                                       cgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGaaa                                                                                      <  2:677836/150‑1 (MQ=255)                                         cgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTc                                                                                   <  1:618891/151‑1 (MQ=255)                                         cgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTc                                                                                   >  1:360987/1‑151 (MQ=255)                                          gAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcg                                                                                  >  1:88546/1‑151 (MQ=255)                                           aGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcgg                                                                                 >  2:464020/1‑151 (MQ=255)                                            ggAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggc                                                                                >  2:829584/1‑151 (MQ=255)                                            ggAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggc                                                                                >  2:846834/1‑151 (MQ=255)                                                 tactCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccg                                                                           >  2:467059/1‑151 (MQ=255)                                                 tactCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccg                                                                           >  1:883504/1‑151 (MQ=255)                                                   ctcctcGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccggc                                                                         >  2:604588/1‑151 (MQ=255)                                                     cctcGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccggca                                                                        <  2:277866/150‑1 (MQ=255)                                                       tcGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCggcatccggcatcc                                                                     >  1:322119/1‑151 (MQ=255)                                                        cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGgcatccggcatccg                                                                    >  2:338099/1‑151 (MQ=255)                                                          cacacaAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCggg                                                                  >  2:750838/1‑151 (MQ=255)                                                          cacacaAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCggg                                                                  >  1:380264/1‑151 (MQ=255)                                                           acacaAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGgc                                                                 <  2:422919/151‑1 (MQ=255)                                                              caAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCt                                                              <  1:339637/151‑1 (MQ=255)                                                                aaaaCGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTa                                                             >  1:432812/1‑150 (MQ=255)                                                                   aCGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAAcgc                                                         <  2:239764/151‑1 (MQ=255)                                                                           aTCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAAcgc                                                 <  2:320485/151‑1 (MQ=255)                                                                              gCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGt                                               >  2:219189/1‑150 (MQ=255)                                                                                 gCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACg                                            <  2:806785/150‑1 (MQ=255)                                                                                   gACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTtg                                         >  1:878831/1‑151 (MQ=255)                                                                                         ggCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGt                                    >  1:661142/1‑150 (MQ=255)                                                                                         ggCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTa                                   <  2:774197/151‑1 (MQ=255)                                                                                             aaTGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTaaa                                 <  2:358115/149‑1 (MQ=255)                                                                                             aaTGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAAtg                               <  1:289050/151‑1 (MQ=255)                                                                                              aTGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAAtgt                              >  1:116272/1‑151 (MQ=255)                                                                                               tGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAAtgtg                             >  2:649785/1‑151 (MQ=255)                                                                                               tGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAAtgtg                             >  1:210467/1‑151 (MQ=255)                                                                                                 cTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTgct                           >  2:94868/1‑151 (MQ=255)                                                                                                   gTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTgct                           <  1:323426/149‑1 (MQ=255)                                                                                                     aCTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTgctggct                       <  2:583320/151‑1 (MQ=255)                                                                                                     aCTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTgctggct                       <  2:481237/151‑1 (MQ=255)                                                                                                         aaTAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCgg                   >  2:719836/1‑151 (MQ=255)                                                                                                         aaTAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCgg                   >  1:485306/1‑151 (MQ=255)                                                                                                              gCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATa              <  1:208388/151‑1 (MQ=255)                                                                                                                   tGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCa          <  1:28430/150‑1 (MQ=255)                                                                                                                       gTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATaaa      <  1:164793/150‑1 (MQ=255)                                                                                                                         tGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGt    <  1:78150/150‑1 (MQ=255)                                                                                                                          ggTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGa  <  1:523919/151‑1 (MQ=255)                                                                                                                                     |                                                                                                                                            GGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGA  >  NC_000913/2181988‑2182259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑