Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,236,037	G→A	G258D (GGT→GAT)	preA →	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,236,037	0	G	A	100.0%	244.7 / NA	64	G258D (GGT→GAT)	preA	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/24);  total (40/24)

CGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGATC  >  NC_000913/2235891‑2236183                                                                                                                                                   |                                                                                                                                                   cGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGatat                                                                                                                                                 <  2:631093/150‑1 (MQ=255)   aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcgg                                                                                                                                              >  2:134008/1‑151 (MQ=255)    tCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcggc                                                                                                                                             >  1:319128/1‑151 (MQ=255)      ttAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcggcgg                                                                                                                                           >  1:613922/1‑151 (MQ=255)       tAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcggcgg                                                                                                                                           <  1:13258/150‑1 (MQ=255)       tAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATc                                                                                                                                                <  2:300242/145‑1 (MQ=255)       tAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCggcggc                                                                                                                                          >  2:892923/1‑151 (MQ=255)         aTCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCAt                                                                                                                                        >  1:79419/1‑151 (MQ=255)           cAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTg                                                                                                                                      <  1:179510/151‑1 (MQ=255)             gAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGa                                                                                                                                     >  1:317703/1‑150 (MQ=255)              aaaaTCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGaa                                                                                                                                    >  2:442303/1‑150 (MQ=255)               aaaTCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAAc                                                                                                                                  <  1:533257/151‑1 (MQ=255)                    ggTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTggg                                                                                                                             <  2:325286/151‑1 (MQ=255)                       aTGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGggagg                                                                                                                          >  2:892045/1‑151 (MQ=255)                       aTGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGggag                                                                                                                           <  1:276295/150‑1 (MQ=255)                        tGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGggagg                                                                                                                          >  1:500932/1‑150 (MQ=255)                         gCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGAt                                                                                                                        <  2:556740/151‑1 (MQ=255)                         gCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGAt                                                                                                                        <  1:550305/151‑1 (MQ=255)                            gATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCg                                                                                                                     >  2:9164/1‑151 (MQ=255)                             aTCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCgg                                                                                                                    >  2:524579/1‑151 (MQ=255)                               cGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCt                                                                                                                  >  1:375998/1‑151 (MQ=255)                                gTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg                                                                                                                 <  1:486542/151‑1 (MQ=255)                                gTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg                                                                                                                 <  2:586620/151‑1 (MQ=255)                                  tAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg                                                                                                                 >  2:894124/1‑149 (MQ=255)                                   aaCGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAg                                                                                                               <  2:72154/150‑1 (MQ=255)                                    aCGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGtt                                                                                                             >  1:380367/1‑151 (MQ=255)                                      ggAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGtttt                                                                                                           >  1:842856/1‑151 (MQ=255)                                          aaTCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTAtt                                                                                                       >  1:395524/1‑151 (MQ=255)                                          aaTCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCAATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTAtt                                                                                                       >  1:408760/1‑151 (MQ=255)                                               aGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTc                                                                                                   >  2:855601/1‑150 (MQ=255)                                                  aTTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGgcg                                                                                               >  1:776180/1‑151 (MQ=255)                                                     tCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCgca                                                                                             <  1:91054/150‑1 (MQ=255)                                                      ccGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCgca                                                                                             <  2:432846/149‑1 (MQ=255)                                                       cGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCgcagc                                                                                           >  2:789121/1‑150 (MQ=255)                                                        ggATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCaa                                                                                         <  1:192636/151‑1 (MQ=255)                                                         gATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCaa                                                                                         >  1:607557/1‑150 (MQ=255)                                                          atatTCCGGAAAAGCGGTAAAACCGATCGCCCTGCGAATCATTAAGCAAATGAGAACCCATCCAGAACTGCGCGATTTCCCAATCATAGATATCGGCGGCATAGAAACCTGGGGGGAATCGGTTGAGTTTTTATTGCCCGGCGCAGCaa                                                                                         >  2:778083/1‑149 (MQ=255)                                                            atTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGtt                                                                                     >  2:842188/1‑151 (MQ=255)                                                                    aaaGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGt                                                                               >  2:685164/1‑149 (MQ=255)                                                                      aGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGacc                                                                           >  1:265911/1‑151 (MQ=255)                                                                              aaCCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGcat                                                                   <  1:685213/151‑1 (MQ=255)                                                                                   aTCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGc                                                              >  1:110496/1‑151 (MQ=255)                                                                                      gCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAg                                                            >  1:675778/1‑150 (MQ=255)                                                                                        ccTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTAc                                                         <  2:894000/151‑1 (MQ=255)                                                                                          tGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACgg                                                       >  1:222291/1‑151 (MQ=255)                                                                                           gcgcTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGCTTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACggg                                                      >  1:92696/1‑151 (MQ=255)                                                                                           gcgcTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACggg                                                      >  1:111484/1‑151 (MQ=255)                                                                                           gcgcTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACggg                                                      >  1:346046/1‑151 (MQ=255)                                                                                           gcgcTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACggg                                                      >  2:523002/1‑151 (MQ=255)                                                                                                tcattcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCg                                                 >  2:535898/1‑151 (MQ=255)                                                                                                 cattcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCg                                                 <  2:90486/150‑1 (MQ=255)                                                                                                     caGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGAt                                              >  1:279302/1‑149 (MQ=255)                                                                                                      aGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGt                                           <  2:897524/151‑1 (MQ=255)                                                                                                      aGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGt                                           <  2:891236/151‑1 (MQ=255)                                                                                                        cAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTgg                                         <  2:279388/151‑1 (MQ=255)                                                                                                           aTGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCCTTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCCGCAACGTTACAGGGGACCACCGGCATCATGCAGGACGGGTATCGGGTAGTgg                                         >  2:479077/1‑148 (MQ=255)                                                                                                             gcgcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGa                                        >  1:839298/1‑147 (MQ=255)                                                                                                              cgcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGata                                   <  2:435654/151‑1 (MQ=255)                                                                                                               gcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGCAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCCGCGCAGCAACGTTACAGGCGACCACCGGCATCATGCAGTACGGGTATCGGCTAGTGGAAGatat                                  >  2:269383/1‑151 (MQ=255)                                                                                                               gcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGatat                                  >  2:750400/1‑151 (MQ=255)                                                                                                                           aaCTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGtt                      >  1:699678/1‑151 (MQ=255)                                                                                                                            aCTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTg                     <  1:34158/151‑1 (MQ=255)                                                                                                                                  cgATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCAt               <  1:295568/151‑1 (MQ=255)                                                                                                                                    aTTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTa             <  1:736586/151‑1 (MQ=255)                                                                                                                                               aGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGATc  >  2:395950/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGATC  >  NC_000913/2235891‑2236183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑