Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,243,790 T→C intergenic (‑138/‑120) folE ← / → yeiG GTP cyclohydrolase I/S‑formylglutathione hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,243,7900TC98.2% 192.8 / NA 56intergenic (‑138/‑120)folE/yeiGGTP cyclohydrolase I/S‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (25/30);  minor base A (1/0);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCTGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCTG  >  NC_000913/2243646‑2243935
                                                                                                                                                |                                                                                                                                                 
aTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCttt                                                                                                                                             >  2:44884/1‑151 (MQ=255)
aTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCttt                                                                                                                                             <  1:410055/151‑1 (MQ=255)
  ggCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtc                                                                                                                                            <  1:704351/150‑1 (MQ=255)
           gATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATcagc                                                                                                                                      >  2:667363/1‑147 (MQ=255)
              ttCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGtt                                                                                                                               >  1:568912/1‑151 (MQ=255)
                  ccTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTaaaa                                                                                                                           <  1:203243/151‑1 (MQ=255)
                  ccTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTaaa                                                                                                                            >  1:11156/1‑150 (MQ=255)
                  ccTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTaaa                                                                                                                            >  2:574812/1‑150 (MQ=255)
                       aGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCg                                                                                                                      >  2:854866/1‑151 (MQ=255)
                       aGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCg                                                                                                                      >  1:594847/1‑151 (MQ=255)
                           gtgACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTa                                                                                                                   <  1:633762/150‑1 (MQ=255)
                             gACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGc                                                                                                                >  1:535836/1‑151 (MQ=255)
                                gCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCaaa                                                                                                             <  1:338829/151‑1 (MQ=255)
                                 ccTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAAt                                                                                                            <  2:288379/151‑1 (MQ=255)
                                 ccTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAAt                                                                                                            <  2:62961/151‑1 (MQ=255)
                                    ccGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATa                                                                                                           <  1:215344/149‑1 (MQ=255)
                                    ccGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATAc                                                                                                          <  1:785674/150‑1 (MQ=255)
                                      gCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGa                                                                                                       <  1:557048/151‑1 (MQ=255)
                                            cGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGaa                                                                                                 <  1:423221/151‑1 (MQ=255)
                                              tGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGaa                                                                                                 >  2:4753/1‑149 (MQ=255)
                                                ggggCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGtt                                                                                             >  1:726797/1‑151 (MQ=255)
                                                ggggCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGtt                                                                                             <  2:153866/151‑1 (MQ=255)
                                                 gggCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGtt                                                                                             >  1:263039/1‑150 (MQ=255)
                                                 gggCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTa                                                                                            <  2:871880/151‑1 (MQ=255)
                                                    cAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACt                                                                                          >  1:451102/1‑150 (MQ=255)
                                                     aaaGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTca                                                                                        <  2:781317/151‑1 (MQ=255)
                                                          tattaAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGc                                                                                    <  1:840678/150‑1 (MQ=255)
                                                             taAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGcaca                                                                                 <  2:263362/150‑1 (MQ=255)
                                                             taAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACAt                                                                                >  2:822188/1‑151 (MQ=255)
                                                              aaGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACAtt                                                                               <  1:301568/151‑1 (MQ=255)
                                                              aaGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACAtt                                                                               <  1:584935/151‑1 (MQ=255)
                                                              aaGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACAt                                                                                <  2:188302/150‑1 (MQ=255)
                                                                  tGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAAt                                                                           >  1:861938/1‑151 (MQ=255)
                                                                     tttACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATaa                                                                         >  2:631972/1‑150 (MQ=255)
                                                                                  tattGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTAtgatga                                                             >  2:768165/1‑149 (MQ=255)
                                                                                  tattGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATgaa                                                            <  2:339635/150‑1 (MQ=255)
                                                                                   attGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTAtgatga                                                             >  1:760498/1‑148 (MQ=255)
                                                                                   attGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCAGCCTTTCATCAGCAAATGTTAAAACATCGTTATGCAAATACGGACGTGACAGTTACTAACAGCACATTGAATAAAAGGTatgatg                                                              >  2:822180/1‑147 (MQ=255)
                                                                                     tgtgCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATgaagaa                                                         >  2:901357/1‑150 (MQ=255)
                                                                                        gCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTg                                                     <  1:649832/151‑1 (MQ=255)
                                                                                           tGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATTAAGAAATTGc                                                    <  2:868562/149‑1 (MQ=255)
                                                                                                 ggcgCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAacaaca                                            <  1:652207/151‑1 (MQ=255)
                                                                                                       caTAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACAcaacaa                                      <  2:352189/151‑1 (MQ=255)
                                                                                                          aaTCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGCTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGa                                   <  2:342899/151‑1 (MQ=255)
                                                                                                              cAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGcc                                >  1:644440/1‑150 (MQ=255)
                                                                                                              cAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCa                               <  1:851768/151‑1 (MQ=255)
                                                                                                              cAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCa                               <  2:594798/151‑1 (MQ=255)
                                                                                                                gTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACg                             <  1:204501/151‑1 (MQ=255)
                                                                                                                   ttCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCAt                          >  1:174157/1‑151 (MQ=255)
                                                                                                                            aTAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCt                 >  2:103684/1‑151 (MQ=255)
                                                                                                                             tAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTc                >  1:196074/1‑151 (MQ=255)
                                                                                                                              aaTTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCg               >  1:782330/1‑151 (MQ=255)
                                                                                                                                  attGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAaga           <  2:177427/151‑1 (MQ=255)
                                                                                                                                      tAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCAc       >  2:118937/1‑151 (MQ=255)
                                                                                                                                       aaTTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCAcc      >  1:67109/1‑151 (MQ=255)
                                                                                                                                       aaTTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCAc       <  1:596945/150‑1 (MQ=255)
                                                                                                                                           gccgccgccTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCTg  >  1:516873/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCTGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCTG  >  NC_000913/2243646‑2243935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑