Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,116	G→A	D93N (GAT→AAT)	nfo →	endonuclease IV with intrinsic 3'‑5' exonuclease activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,116	0	G	A	100.0%	322.0 / NA	85	D93N (GAT→AAT)	nfo	endonuclease IV with intrinsic 3'‑5' exonuclease activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/35);  total (50/35)

GCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACA  >  NC_000913/2250968‑2251262                                                                                                                                                     |                                                                                                                                                   gCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAAt                                                                                                                                                  >  2:446670/1‑151 (MQ=255)    cACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGaa                                                                                                                                               <  1:727618/151‑1 (MQ=255)    cACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGa                                                                                                                                                >  1:636759/1‑150 (MQ=255)      cgacgCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAAt                                                                                                                                             <  1:360917/151‑1 (MQ=255)          gCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGc                                                                                                                                           >  2:303383/1‑149 (MQ=255)          gCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAg                                                                                                                                         <  2:315835/151‑1 (MQ=255)           cAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGc                                                                                                                                        >  1:146560/1‑151 (MQ=255)           cAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGc                                                                                                                                        >  1:19319/1‑151 (MQ=255)                   cGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATACCTTTATAAATGAAATGCAGCGTTGCGa                                                                                                                                 >  1:584882/1‑150 (MQ=255)                    gATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGaa                                                                                                                                >  2:572653/1‑150 (MQ=255)                        aaTTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCt                                                                                                                           >  2:698471/1‑151 (MQ=255)                        aaTTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCt                                                                                                                           >  2:495545/1‑151 (MQ=255)                            cAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTgggg                                                                                                                       <  2:787563/151‑1 (MQ=255)                               aGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGctt                                                                                                                    >  1:744238/1‑151 (MQ=255)                                  cgccTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGctttct                                                                                                                 >  2:315003/1‑151 (MQ=255)                                     cTGTGAAAAATATCACTACGCAGCGGCGCAAAGTCATCCCCACGACAGTTATCTGATTAACATCCGACATCCGGTCACTGAAGCTCTGCAAAAATCGCGCGATGCCTATATAAATGAAAAGCAGCGTTGCGAACAGCTGGGGctttcttt                                                                                                               <  2:823867/150‑1 (MQ=255)                                     cTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTg                                                                                                              >  2:23590/1‑151 (MQ=255)                                       gtgAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCt                                                                                                            >  1:899947/1‑151 (MQ=255)                                       gtgAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGAGTTGCGAACAGCTGGGGCTTTCTTTGCt                                                                                                            >  2:597415/1‑151 (MQ=255)                                         gAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCa                                                                                                          <  2:784236/151‑1 (MQ=255)                                          aaaaaTATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCa                                                                                                          >  2:176137/1‑150 (MQ=255)                                             aaTATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATACCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACtt                                                                                                      >  2:604037/1‑151 (MQ=255)                                             aaTATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCACGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGTGCTTTCTTTGCTCAACtt                                                                                                      <  1:765347/151‑1 (MQ=255)                                                atCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTcc                                                                                                    >  2:29545/1‑150 (MQ=255)                                                  cACTCCACATCGGCGCAACTTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCAcc                                                                                                 >  2:705454/1‑151 (MQ=255)                                                     tACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTg                                                                                              >  2:335279/1‑151 (MQ=255)                                                      acacATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTgg                                                                                             <  2:557180/151‑1 (MQ=255)                                                      acacATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTgg                                                                                             <  2:169228/151‑1 (MQ=255)                                                      acacATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTg                                                                                              <  1:37364/150‑1 (MQ=255)                                                       cacaTCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGc                                                                                            <  1:590298/151‑1 (MQ=255)                                                       cacaTCGGCGCAAATTCTTCCACAAGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCt                                                                                               >  2:227536/1‑148 (MQ=255)                                                         caTCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGc                                                                                            >  2:866221/1‑149 (MQ=255)                                                         caTCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGc                                                                                            >  1:864612/1‑149 (MQ=255)                                                            cGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGcc                                                                                        >  2:770503/1‑150 (MQ=255)                                                            cGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGcc                                                                                        >  1:716453/1‑150 (MQ=255)                                                               cgcAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCt                                                                                    >  1:327237/1‑151 (MQ=255)                                                                  aaaTTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGAt                                                                                 >  2:390954/1‑151 (MQ=255)                                                                    aTTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGc                                                                               >  1:380857/1‑151 (MQ=255)                                                                    aTTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGc                                                                               <  2:386032/151‑1 (MQ=255)                                                                     ttcttcCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGc                                                                               >  2:821047/1‑150 (MQ=255)                                                                        ttcCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGc                                                                               >  2:552472/1‑147 (MQ=255)                                                                         tcCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAg                                                                             <  1:503012/148‑1 (MQ=255)                                                                         tcCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGa                                                                            <  1:182275/149‑1 (MQ=255)                                                                          ccccACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGAttt                                                                         >  1:870026/1‑151 (MQ=255)                                                                            ccACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTc                                                                        >  2:543825/1‑150 (MQ=255)                                                                                gACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCaga                                                                     >  2:617491/1‑149 (MQ=255)                                                                                   aGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAgg                                                                <  1:314092/151‑1 (MQ=255)                                                                                   aGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAgg                                                                <  1:543875/151‑1 (MQ=255)                                                                                   aGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAgg                                                                >  2:433039/1‑151 (MQ=255)                                                                                       aTCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGAtt                                                             <  2:858958/150‑1 (MQ=255)                                                                                           gATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTg                                                            <  2:400178/147‑1 (MQ=255)                                                                                           gATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCtt                                                        <  1:181824/151‑1 (MQ=255)                                                                                           gATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCt                                                         <  2:162096/150‑1 (MQ=255)                                                                                              tAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTgcg                                                     >  1:365616/1‑151 (MQ=255)                                                                                               aaCCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTgcgc                                                    >  2:303866/1‑151 (MQ=255)                                                                                               aaCCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTgcgc                                                    >  2:580414/1‑151 (MQ=255)                                                                                               aaCCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTgcgc                                                    >  2:419686/1‑151 (MQ=255)                                                                                                  cTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGt                                                  >  2:719428/1‑150 (MQ=255)                                                                                                     ggACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAAGGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTg                                              <  2:660522/151‑1 (MQ=255)                                                                                                       aCATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGcc                                            <  1:27650/151‑1 (MQ=255)                                                                                                        cATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCg                                           <  1:124587/151‑1 (MQ=255)                                                                                                           ccGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAAt                                        >  2:796396/1‑151 (MQ=255)                                                                                                            cGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATc                                       <  1:749992/151‑1 (MQ=255)                                                                                                              gTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCa                                     <  2:401839/151‑1 (MQ=255)                                                                                                                cACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCAt                                    <  2:646101/150‑1 (MQ=255)                                                                                                                  cTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCa                                  >  1:232825/1‑150 (MQ=255)                                                                                                                     aaGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACAt                              <  1:809586/151‑1 (MQ=255)                                                                                                                        ctctGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTgc                           >  2:78657/1‑151 (MQ=255)                                                                                                                          ctGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTgc                           <  2:407739/149‑1 (MQ=255)                                                                                                                            ggAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTgcg                          <  2:284879/148‑1 (MQ=255)                                                                                                                            ggAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCt                        <  1:714923/150‑1 (MQ=255)                                                                                                                                aaaTCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCTCCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGGCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGaga                   <  2:107764/151‑3 (MQ=255)                                                                                                                                aaaTCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGAt                    >  2:12237/1‑150 (MQ=255)                                                                                                                                  aTCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATaaa                 <  1:650111/151‑1 (MQ=255)                                                                                                                                      cgcgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTc             <  1:715463/151‑1 (MQ=255)                                                                                                                                      cgcgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTc             >  2:653602/1‑151 (MQ=255)                                                                                                                                           tGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAgg         >  2:5029/1‑150 (MQ=255)                                                                                                                                           tGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAgg         >  2:543476/1‑150 (MQ=255)                                                                                                                                             ccTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATaaa                 >  1:222670/1‑140 (MQ=255)                                                                                                                                             ccTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATaaa                 <  2:222644/140‑1 (MQ=255)                                                                                                                                               tttATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTTCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGtgtg     >  2:354723/1‑150 (MQ=255)                                                                                                                                                 tataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGAc   >  2:749056/1‑150 (MQ=255)                                                                                                                                                 tataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGAc   >  2:108994/1‑150 (MQ=255)                                                                                                                                                  ataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGAc   >  1:744629/1‑149 (MQ=255)                                                                                                                                                  ataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACa  <  1:317828/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACA  >  NC_000913/2250968‑2251262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑