| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,265,230 | A→G | G22G (GGT→GGC) | yeiW ← | UPF0153 cysteine cluster protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,265,230 | 0 | A | G | 95.1% | 111.5 / ‑5.2 | 42 | G22G (GGT→GGC) | yeiW | UPF0153 cysteine cluster protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/14); minor base T (2/0); total (28/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
TGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACC > NC_000913/2265087‑2265377 | tgctgACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGGCggg > 1:611712/1‑149 (MQ=255) tACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGATAGGGGTGGgaa > 2:131585/1‑150 (MQ=255) aCCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCCTCTGGCATGCCGGGGATAGGGGTGGgaa > 2:698430/1‑149 (MQ=255) ccGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTg < 2:340169/151‑1 (MQ=255) cacaTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGATAGGGCTGGGAATTGaa > 1:683784/1‑150 (MQ=255) tttCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGATAGGGCTGGGAATTGAAgggg > 1:177174/1‑150 (MQ=255) cAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGt < 1:147480/151‑1 (MQ=255) cAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGt < 2:191788/151‑1 (MQ=255) aGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTg < 1:315366/151‑1 (MQ=255) cgcTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCa < 2:376784/151‑1 (MQ=255) cTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGaaa < 1:447903/133‑1 (MQ=255) tGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGCCATCTGGCCAGCCGGGGCTAGGGGTGGGAATTTAAGGGGCCGGGCaa > 2:39315/1‑149 (MQ=255) aGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGATAGGGCTGGGAATTTAAGGGGCAGCGCAACACGcccccc > 2:713178/1‑148 (MQ=255) gCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCATCTGGCATGCCGGGGATAGGGCTGGGAATTGAAGGGGCAGTGCAACACGCCCCGCAAccc > 1:286919/1‑150 (MQ=255) aCTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTCGCCTCTGGGTTTACGGGGATTGGGGTGGGGATTTAAGGGGGCGGGCACCACCCCCCGCAAccccggccg > 2:240092/1‑144 (MQ=255) ttCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGGATGCCGGGGATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑Att > 1:877228/1‑151 (MQ=255) cAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACgt < 1:250948/151‑1 (MQ=255) aGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCCTCTGGCATGCCGGGGATAGGGGTGGGAATTGAAGGGGCAGTGCAAAACGCCCCGCAACCCGGGCGGC‑ATTCCATACgtt > 1:507919/1‑151 (MQ=255) cGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCGATCCGGGGATAGGGGTGGAAATTGTAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTTCCTACgttgt > 1:877691/1‑148 (MQ=255) gTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAAAAGGGCTGGAAATTGAAGGGGCAGTGCAAAACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCtt > 2:718157/1‑150 (MQ=255) gAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCATCTGGCATGCCGGGGATAGGGGTGGGAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCtt > 2:838014/1‑148 (MQ=255) aaaTTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGt < 1:776057/151‑1 (MQ=255) cAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGCATTGTCCACTCTGAGCCCGGGGAAAGGGCTGGAAATTTAAGGGGGGGCGCAAAACGCCCCGCAACCCGGACGGC‑ATTACATATGTTCTTTTCTTAAgggtta > 2:207816/1‑142 (MQ=255) gTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGGAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGAt > 1:78652/1‑151 (MQ=255) gCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCCTCTGGCATGCCGGGGAAAGGGCCGGAAATTGAAGGGGCAGGGCAACAAGCCCCCGCACCCGGCAGGCTATTCC‑TTCGTTGTTTTCCTTAGGGTAACCACCCGAAtg > 1:651641/1‑150 (MQ=255) gCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATtga < 2:877157/151‑1 (MQ=255) tGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGTCCTCTGGCATGCCGGGGATAGGGCTGGGAAATGAAGGGGCAGTGCAAAACGCCCCGCAACCCGGACGGG‑CTTCCATACGGTGTTCTCTTAAGGGTAACCACCGGAtt > 2:738797/1‑147 (MQ=255) tGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGGTAACCACCGGATtgatg > 1:302555/1‑151 (MQ=255) ttCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGGTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGATAACCACCGGATtgatg > 2:163464/1‑149 (MQ=255) catcaAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCATCTGGCATGCCGGGAATAGGGGTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCCACCCGGGCGGC‑ATTCCATACGTTGGTCTTTTAAGGTTAACCACCGGATtgat > 2:182614/1‑146 (MQ=255) caAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAgcgcg < 1:92071/150‑1 (MQ=255) tGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGcaca > 1:140805/1‑149 (MQ=255) gAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAg < 2:434701/149‑1 (MQ=255) gCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTacca < 1:596155/150‑1 (MQ=255) aGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTaccacc > 1:415188/1‑150 (MQ=255) gTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCtttttt < 1:898680/151‑1 (MQ=255) aTTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTg < 2:762340/150‑1 (MQ=255) ggCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGc > 2:23034/1‑150 (MQ=255) gCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAg > 2:178051/1‑150 (MQ=255) aTCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCg > 2:492138/1‑151 (MQ=255) tGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTACACCAGCAAAAGTGCGAAt > 1:438000/1‑151 (MQ=255) cATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATAcc > 1:71378/1‑151 (MQ=255) | TGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGC‑ATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACC > NC_000913/2265087‑2265377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |