Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,287,209 A→G pseudogene (1706/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,287,2090AG97.5% 124.9 / NA 40pseudogene (1706/2525 nt)yejOpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (20/19);  minor base C (0/1);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCACGGGAGAAACGGCTATCGATACCGAGCGTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTACCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTATCCAGCAAGGTA  >  NC_000913/2287065‑2287356
                                                                                                                                                |                                                                                                                                                   
ttcACGGGAGAAACGGCTATCGATACCGAGCGTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCa                                                                                                                                                  >  2:163808/1‑148 (MQ=255)
   aCGGGAGAAACGGCTATCGATACCGAGCGTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGc                                                                                                                                            >  2:885432/1‑151 (MQ=255)
     gggAGAAACGGCTATCGATACCGAGCGTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTc                                                                                                                                          >  2:686170/1‑151 (MQ=255)
             cGGCTATCGATACCGAGCGTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACgg                                                                                                                                  >  2:162177/1‑151 (MQ=255)
               gCTATCGATACCGAGCGTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGgtg                                                                                                                                <  2:519015/151‑1 (MQ=255)
                               gTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCa                                                                                                                 >  2:208610/1‑150 (MQ=255)
                                  aGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATa                                                                                                             <  2:442929/151‑1 (MQ=255)
                                    gCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATAcc                                                                                                           >  2:280487/1‑151 (MQ=255)
                                          cAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCgg                                                                                                      <  1:789018/150‑1 (MQ=255)
                                                     cAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGgccgc                                                                                          >  2:511706/1‑151 (MQ=255)
                                                           cAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATAtt                                                                                    <  1:38415/151‑1 (MQ=255)
                                                            aGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATAGCAGCGGTTGTGCGGCCGCCATAtt                                                                                    >  2:265226/1‑150 (MQ=255)
                                                             gCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTc                                                                                   >  2:332579/1‑150 (MQ=255)
                                                                   gCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACAt                                                                            >  2:462519/1‑151 (MQ=255)
                                                                        aGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCCCATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGt                                                                        <  1:660375/150‑1 (MQ=255)
                                                                        aGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTg                                                                       >  1:68721/1‑151 (MQ=255)
                                                                                 gttgttGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAggg                                                              >  2:529288/1‑151 (MQ=255)
                                                                                  ttgttgCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAgggg                                                             >  1:887305/1‑151 (MQ=255)
                                                                                    gttgCGTGTGTTAATTGCCAAACTCCCACTGTCCGTACCGTAACGACCTCCTATGACGCTGCCAAGACGCTCACGCACGTTGTCCAGTTCTGCATCAAATACCGGCTGTTGGGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGt                                                            <  2:724091/150‑1 (MQ=255)
                                                                                    gttgCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGt                                                            <  1:157840/150‑1 (MQ=255)
                                                                                        cGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAAttt                                                       >  2:153860/1‑151 (MQ=255)
                                                                                          ggTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTAACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCGGGGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTgc                                                     <  2:632372/151‑1 (MQ=255)
                                                                                                   tGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGAtt                                             <  1:851301/150‑1 (MQ=255)
                                                                                                     ccGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCCCGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATtct                                           <  2:380737/150‑1 (MQ=255)
                                                                                                     ccGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATtc                                            <  2:887976/149‑1 (MQ=255)
                                                                                                      cGAACTCCACATCGCCGTATCGTAACTACCGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGGGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTctct                                         <  2:628883/151‑1 (MQ=255)
                                                                                                       gagcTCCACATCGCCGTATCTTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTctct                                         <  2:240486/147‑1 (MQ=255)
                                                                                                            ccACATCGCCGTATTGTAACTAACTCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCAGCAAATACCAGCGTTTGTGCGACCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGa                                   <  1:353842/151‑1 (MQ=255)
                                                                                                            ccACATCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGa                                   <  2:418510/151‑1 (MQ=255)
                                                                                                              acaTCGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACt                                 >  1:592699/1‑151 (MQ=255)
                                                                                                                  cGCCGTATCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTcc                               >  2:440156/1‑149 (MQ=255)
                                                                                                                        aTCGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATgg                       >  2:345848/1‑151 (MQ=255)
                                                                                                                          cGTAACTAACGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGAACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGtt                     >  1:244760/1‑151 (MQ=255)
                                                                                                                             aactaacGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATCCCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGc                   <  1:465163/150‑1 (MQ=255)
                                                                                                                                 aacGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTa              <  1:390156/151‑1 (MQ=255)
                                                                                                                                  acGCCTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTAt             >  1:759526/1‑151 (MQ=255)
                                                                                                                                     ccTTTTACGCTCCCAAGACGCTCACGCAGGGGGTCCAGTTCTGCAGCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTATcc           <  2:62481/150‑1 (MQ=255)
                                                                                                                                      cTTTTACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTATcc           >  2:444081/1‑149 (MQ=255)
                                                                                                                                        tttACGCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTATCCAg         <  1:280513/149‑1 (MQ=255)
                                                                                                                                             gCTGCCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTATCCAGCAAGGta  <  1:492805/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TTCACGGGAGAAACGGCTATCGATACCGAGCGTCAGGCCCGTCAATGTTTGCTCAAAACCAGCTCCCGCATCAGTGGTCACGTTGTTGCGGGTGTTAATTGCCGAACTCCACATCGCCGTATCGTAACTAACGCCTTTTACGCTACCAAGACGCTCACGCACGGTGTCCAGTTCTGCATCAAATACCAGCGGTTGTGCGGCCGCCATATTCAGCACATCAGTGGTTGAAGGGGTAATTTGCGCGCGATTCTCTGCCAGACTCCAGCTATGGTTGCCATTATCCAGCAAGGTA  >  NC_000913/2287065‑2287356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: