| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,355,663 | T→C | L48P (CTG→CCG) | glpC → | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,355,663 | 0 | T | C | 98.1% | 180.6 / ‑3.8 | 52 | L48P (CTG→CCG) | glpC | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (21/30); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGT > NC_000913/2355515‑2355808 | ccAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGt > 1:499905/1‑151 (MQ=255) caaTGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATg > 1:499602/1‑150 (MQ=255) tgaatgaCACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACg > 1:416283/1‑150 (MQ=255) acacCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCt > 1:108616/1‑151 (MQ=255) cacCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCt < 1:240584/150‑1 (MQ=255) cacCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTg < 2:260954/151‑1 (MQ=255) cacCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTg < 1:477827/151‑1 (MQ=255) ccAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTATGACGAGGCGCTGa > 1:409642/1‑150 (MQ=255) ccAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGaa < 1:367656/151‑1 (MQ=255) cAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGaa > 1:729455/1‑150 (MQ=255) cAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGaa > 2:310724/1‑150 (MQ=255) aGCTTCGAAAACTGCATTAAGTGCACCGTCTGAACCCCCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGaaa < 2:128263/150‑1 (MQ=255) aGCTTCGAAAACTGCATTAAGGGCACCGTCGGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAat < 2:334545/151‑1 (MQ=255) gCTTCGAAAACTGCATTAAGTGCACCTTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAat < 1:713671/150‑1 (MQ=255) gCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAata < 1:882059/151‑1 (MQ=255) gCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAata < 2:728064/151‑1 (MQ=255) ttCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATAtt > 1:320507/1‑151 (MQ=255) ttCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATAtt > 2:568502/1‑151 (MQ=255) tCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTg > 1:214798/1‑151 (MQ=255) gAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTg < 1:412526/149‑1 (MQ=255) aaaaCTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCAt > 1:199832/1‑151 (MQ=255) gCATTAAGTGCACCGTCTGCCCCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACt < 2:428702/150‑1 (MQ=255) gCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTg < 1:408867/151‑1 (MQ=255) cATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGc < 1:600532/151‑1 (MQ=255) gTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGt < 2:828079/151‑1 (MQ=255) tGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGtt > 2:7841/1‑151 (MQ=255) tGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCg > 2:159768/1‑151 (MQ=255) accaccGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGcc < 1:512958/150‑1 (MQ=255) accGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGcc > 2:721152/1‑151 (MQ=255) ccGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGccc > 1:515901/1‑151 (MQ=255) ccGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGcc < 1:274243/150‑1 (MQ=255) cGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCg > 2:823015/1‑151 (MQ=255) cccGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGAt < 2:339393/151‑1 (MQ=255) ccGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGAtg < 2:514657/151‑1 (MQ=255) ccGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGAtt > 1:769176/1‑151 (MQ=255) gAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGat > 2:870526/1‑151 (MQ=255) gTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCa < 2:584836/151‑1 (MQ=255) tATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAg < 2:689946/150‑1 (MQ=255) ccAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAgcgcg < 1:351285/151‑1 (MQ=255) aGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAgcgcgcg < 2:43930/151‑1 (MQ=255) cAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGc > 1:437895/1‑151 (MQ=255) aaaaCAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGGATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCg > 2:848954/1‑151 (MQ=255) aaCAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCTCGCGGGCGa < 1:773740/150‑1 (MQ=255) aCAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGaaa < 2:425144/151‑1 (MQ=255) aGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAatat < 2:659197/151‑1 (MQ=255) gggccggATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGaca < 2:215859/151‑1 (MQ=255) cGAGCGTCTGCGTTTGAAAGATGGCGAACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACgcgc > 1:52844/1‑148 (MQ=255) tGAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAAtt < 1:196325/150‑1 (MQ=255) gAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAAtttt > 2:181560/1‑151 (MQ=255) gAAAGATGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAAtttt < 1:417731/151‑1 (MQ=255) aTGGCGCACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGtt < 2:879512/151‑1 (MQ=255) cgcACCGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGt < 1:88145/151‑1 (MQ=255) | CCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTGCACCACCGCCTGCCCGGTGAGCCGGGTGAATCCCGGTTATCCAGGGCCAAAACAAGCCGGGCCGGATGGCGAGCGTCTGCGTTTGAAAGATGGCGCACTGTATGACGAGGCGCTGAAATATTGCATCAACTGCAAACGTTGTGAAGTCGCCTGCCCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGT > NC_000913/2355515‑2355808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |