Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,634	T→C	Q511R (CAG→CGG)	nuoG ←	NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,634	0	T	C	100.0%	158.6 / NA	47	Q511R (CAG→CGG)	nuoG	NADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/19);  total (28/19)

GCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGG  >  NC_000913/2398486‑2398781                                                                                                                                                     |                                                                                                                                                    gcgTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGa                                                                                                                                                   <  2:430902/151‑1 (MQ=255)   gTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAAt                                                                                                                                                 <  2:817922/151‑1 (MQ=255)   gTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAAt                                                                                                                                                 >  2:299141/1‑151 (MQ=255)   gTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAAt                                                                                                                                                 >  2:250632/1‑151 (MQ=255)   gTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAG‑CCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATc                                                                                                                                                >  2:522999/1‑151 (MQ=255)    tCCGGTTTCCAGTTCGGTTAACGCTTCTTCTAGCGTACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGCCGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTATCCGCCGCCCGAATc                                                                                                                                                >  2:147574/1‑151 (MQ=255)     ccGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATc                                                                                                                                                >  1:885325/1‑150 (MQ=255)       ggTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCAcc                                                                                                                                             >  1:105569/1‑151 (MQ=255)          ttCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACctc                                                                                                                                           <  2:335765/150‑1 (MQ=255)           tCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACctct                                                                                                                                          >  2:62745/1‑150 (MQ=255)           tCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACctct                                                                                                                                          >  1:567567/1‑150 (MQ=255)                ttCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCt                                                                                                                                     >  1:534171/1‑150 (MQ=255)                  cGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTAcc                                                                                                                                  >  2:395356/1‑151 (MQ=255)                      tAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCg                                                                                                                              >  1:306631/1‑151 (MQ=255)                          gCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCg                                                                                                                          >  1:758301/1‑151 (MQ=255)                             tcttcAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTcc                                                                                                                       >  1:800478/1‑151 (MQ=255)                                 cAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGga                                                                                                                   <  1:507095/151‑1 (MQ=255)                                      gAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATaa                                                                                                              <  2:479916/151‑1 (MQ=255)                                               cccATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGtt                                                                                                     >  1:308926/1‑151 (MQ=255)                                                           aGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTg                                                                                         >  1:515933/1‑151 (MQ=255)                                                                  tGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCa                                                                                      >  2:491041/1‑147 (MQ=255)                                                                         gACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACg                                                                           <  1:283239/151‑1 (MQ=255)                                                                            ggAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATg                                                                        >  1:692940/1‑151 (MQ=255)                                                                               aCGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACg                                                                     >  1:265753/1‑151 (MQ=255)                                                                                 gggCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTc                                                                   >  1:543467/1‑151 (MQ=255)                                                                                  ggCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCg                                                                  <  1:313991/151‑1 (MQ=255)                                                                                   gCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACg                                                                           >  1:696955/1‑141 (MQ=255)                                                                                   gCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACg                                                                           <  2:696906/141‑1 (MQ=255)                                                                                    cAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGAt                                                                <  2:745993/151‑1 (MQ=255)                                                                                      aTCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGAttt                                                              >  1:320775/1‑151 (MQ=255)                                                                                          tGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttgct                                                          >  1:97268/1‑151 (MQ=255)                                                                                               aTACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttgctttgc                                                      >  1:510322/1‑150 (MQ=255)                                                                                                 aCCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAg                                                    <  2:306605/150‑1 (MQ=255)                                                                                                         cAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTc                                           <  1:166860/151‑1 (MQ=255)                                                                                                           gcgcCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGa                                         <  2:124705/151‑1 (MQ=255)                                                                                                                  gACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTc                                  >  1:434272/1‑151 (MQ=255)                                                                                                                       ttCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAAcc                              <  1:712389/150‑1 (MQ=255)                                                                                                                             gCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTg                       <  2:110224/151‑1 (MQ=255)                                                                                                                                    cgACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGtt                >  2:236782/1‑151 (MQ=255)                                                                                                                                          tAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCa           <  1:150654/150‑1 (MQ=255)                                                                                                                                           aGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAg          <  2:203771/150‑1 (MQ=255)                                                                                                                                                ccgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATg    >  1:663204/1‑151 (MQ=255)                                                                                                                                                ccgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATg    <  1:644937/151‑1 (MQ=255)                                                                                                                                                 cgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATgg   >  1:336121/1‑151 (MQ=255)                                                                                                                                                 cgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATgg   <  2:544188/151‑1 (MQ=255)                                                                                                                                                 cgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATgg   >  2:798294/1‑151 (MQ=255)                                                                                                                                                  gccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATggg  <  1:417685/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGG  >  NC_000913/2398486‑2398781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑