Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,401,795	G→A	R86R (CGC→CGT)	nuoE ←	NADH:ubiquinone oxidoreductase, chain E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,401,795	0	G	A	100.0%	172.9 / NA	46	R86R (CGC→CGT)	nuoE	NADH:ubiquinone oxidoreductase, chain E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/17);  total (29/17)

CCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAG  >  NC_000913/2401648‑2401935                                                                                                                                                    |                                                                                                                                             ccccAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACg                                                                                                                                            <  1:447979/150‑1 (MQ=255)       gcagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAAcc                                                                                                                                     >  1:592403/1‑151 (MQ=255)               ttGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCg                                                                                                                             >  2:422578/1‑151 (MQ=255)                 ggCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCgg                                                                                                                            <  1:475419/150‑1 (MQ=255)                  gcagcaGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGaa                                                                                                                          <  2:592354/151‑1 (MQ=255)                  gcagcaGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGa                                                                                                                           >  2:130756/1‑150 (MQ=255)                    agcagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAg                                                                                                                         <  2:484393/150‑1 (MQ=255)                          gTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGAc                                                                                                                  >  2:857921/1‑151 (MQ=255)                           tAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACt                                                                                                                 >  2:389740/1‑151 (MQ=255)                            aaaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTg                                                                                                                <  2:364719/151‑1 (MQ=255)                             aaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGt                                                                                                               >  2:434407/1‑151 (MQ=255)                             aaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGt                                                                                                               <  2:199381/151‑1 (MQ=255)                                        aaaTGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGcc                                                                                                    >  1:45753/1‑151 (MQ=255)                                         aaTGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGcc                                                                                                    >  1:679611/1‑150 (MQ=255)                                          aTGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCcac                                                                                                  >  2:40757/1‑151 (MQ=255)                                              cGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACAcc                                                                                               <  2:227116/150‑1 (MQ=255)                                               gTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCtt                                                                                             >  2:462018/1‑151 (MQ=255)                                                tttGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGTCCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTc                                                                                            >  1:193241/1‑151 (MQ=255)                                                           ttGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTg                                                                                 >  1:764912/1‑151 (MQ=255)                                                            tGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGc                                                                                >  1:46030/1‑151 (MQ=255)                                                                 ttCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGa                                                                            >  1:9432/1‑150 (MQ=255)                                                                   cAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAAt                                                                          >  2:463476/1‑150 (MQ=255)                                                                       tttttCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATAccc                                                                      >  2:564344/1‑150 (MQ=255)                                                                       tttttCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATAccc                                                                      >  1:494215/1‑150 (MQ=255)                                                                              cGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGcac                                                                 <  2:133175/148‑1 (MQ=255)                                                                              cGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACAt                                                               <  1:396408/150‑1 (MQ=255)                                                                                   gccgccTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCg                                                          >  2:421497/1‑150 (MQ=255)                                                                                     cgccTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATc                                                       >  2:383098/1‑151 (MQ=255)                                                                                          gAATGCCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATcgcg                                                    <  2:737640/149‑1 (MQ=255)                                                                                          gAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGt                                                   <  2:316908/150‑1 (MQ=255)                                                                                            aTACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGa                                                <  1:881100/151‑1 (MQ=255)                                                                                                    aTAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCa                                        <  1:800844/151‑1 (MQ=255)                                                                                                       aCCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATcc                                     >  1:81262/1‑151 (MQ=255)                                                                                                        ccGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCg                                    <  1:346995/151‑1 (MQ=255)                                                                                                             gATATGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCAcc                               >  1:893130/1‑151 (MQ=255)                                                                                                                atGACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCAccc                              >  1:467792/1‑149 (MQ=255)                                                                                                                  gACAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGcc                          >  1:792457/1‑151 (MQ=255)                                                                                                                   aCAGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCa                         <  1:7567/151‑1 (MQ=255)                                                                                                                     aGACCACGCTGTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACg                       >  1:676954/1‑151 (MQ=255)                                                                                                                               gTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCt               <  1:383124/149‑1 (MQ=255)                                                                                                                               gTCACAATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCt               <  2:893059/149‑1 (MQ=255)                                                                                                                                 cacaATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAAc           >  1:575259/1‑151 (MQ=255)                                                                                                                                 cacaATAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAAc           >  2:775820/1‑151 (MQ=255)                                                                                                                                    aaTAACGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGAt        >  2:85120/1‑151 (MQ=255)                                                                                                                                         cGGATCACATGACGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTc    >  2:66003/1‑150 (MQ=255)                                                                                                                                           gATCACATGACGACCCACCGGCAGGCGGAAGATATGACTGAAGAACGTTGCAACACCTTCGACGGCGCTTGCCGGAATACCCAGCACATCAGCGATCGCGAGGATCGCACCATCCGGCCCCCAGCCACGCTGCTTCTGAACGATTTTCAg  >  2:638267/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                             CCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGATCACATGGCGACCAACCGGCTGGCGGAAGATCTGACTGTAGAACGTTGCCACACCTTCGACGTCGCTTGCCGGAATACCCAGCACATCGGCGATCGCGTGGATCGCACCATCCGGCACCCAGCCACGCTGCTTCTGAACGATTTTCAG  >  NC_000913/2401648‑2401935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑