Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,445,152	G→A	R137C (CGC→TGC)	yfcA ←	TauE/TSUP family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,445,152	0	G	A	100.0%	215.2 / NA	55	R137C (CGC→TGC)	yfcA	TauE/TSUP family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

GCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATG  >  NC_000913/2445004‑2445297                                                                                                                                                     |                                                                                                                                                  gCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACt                                                                                                                                                 <  2:252099/151‑1 (MQ=255)  cGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTg                                                                                                                                                >  1:511092/1‑151 (MQ=255)   gTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTgg                                                                                                                                               <  1:632313/151‑1 (MQ=255)       gCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGt                                                                                                                                           <  1:685267/151‑1 (MQ=255)           tGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGtctt                                                                                                                                        >  2:340533/1‑150 (MQ=255)            ggCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGtcttc                                                                                                                                       <  1:171153/150‑1 (MQ=255)                 tGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCAcc                                                                                                                                 >  2:382985/1‑151 (MQ=255)                  ggCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCAcc                                                                                                                                 >  2:621071/1‑150 (MQ=255)                   gCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTa                                                                                                                               <  2:471787/151‑1 (MQ=255)                     gAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGc                                                                                                                             <  1:280285/151‑1 (MQ=255)                       ggTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCCCCTAGcat                                                                                                                           >  2:395761/1‑149 (MQ=255)                       ggTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCtt                                                                                                                           <  2:812257/151‑1 (MQ=255)                          tAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTgg                                                                                                                        >  1:419256/1‑151 (MQ=255)                           aaaTCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGc                                                                                                                       >  1:391821/1‑151 (MQ=255)                                     aGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCa                                                                                                                >  1:292562/1‑148 (MQ=255)                                         tAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCCCGTTTTGGCATCAGccatcaa                                                                                                           >  2:743106/1‑143 (MQ=255)                                          aaCGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATa                                                                                                         >  2:551777/1‑150 (MQ=255)                                           aCGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGaaa                                                                                                           >  1:384127/1‑147 (MQ=255)                                             gAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAgg                                                                                                     <  2:338790/151‑1 (MQ=255)                                              aaGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGc                                                                                                    <  1:750875/151‑1 (MQ=255)                                               aGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAgg                                                                                                     >  2:328417/1‑149 (MQ=255)                                                  ccAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGAt                                                                                                <  2:474089/151‑1 (MQ=255)                                                         gTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAAtt                                                                                         <  2:92022/151‑1 (MQ=255)                                                            aaaCGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTAcc                                                                                      <  2:822580/151‑1 (MQ=255)                                                               cGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGa                                                                                   <  1:730678/151‑1 (MQ=255)                                                                    cGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATggg                                                                              >  1:34612/1‑151 (MQ=255)                                                                       cAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCaa                                                                           <  1:643263/151‑1 (MQ=255)                                                                         gCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCaaaa                                                                         <  2:879477/151‑1 (MQ=255)                                                                             ggCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTg                                                                     <  2:812772/151‑1 (MQ=255)                                                                                cAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGcc                                                                   >  2:412645/1‑150 (MQ=255)                                                                                cAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCg                                                                  >  1:807091/1‑151 (MQ=255)                                                                                    gAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCACCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGcc                                                                   >  2:355665/1‑146 (MQ=255)                                                                                          atcatAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCa                                                        <  1:153416/151‑1 (MQ=255)                                                                                          atcatAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCa                                                        <  1:567245/151‑1 (MQ=255)                                                                                            catAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAg                                                       >  1:635807/1‑150 (MQ=255)                                                                                               aaaaaCCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTg                                                   <  1:367057/151‑1 (MQ=255)                                                                                                  aaCCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGaa                                                 >  2:401579/1‑150 (MQ=255)                                                                                                       aCGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTAtt                                           <  2:878002/151‑1 (MQ=255)                                                                                                       aCGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTAtt                                           <  2:641646/151‑1 (MQ=255)                                                                                                           aGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTAtt                                           >  2:235603/1‑147 (MQ=255)                                                                                                           aGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCAc                                       >  1:418439/1‑151 (MQ=255)                                                                                                              cACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACcag                                    <  1:49325/151‑1 (MQ=255)                                                                                                                 cGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACcagcag                                 <  1:526849/151‑1 (MQ=255)                                                                                                                    cTATTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTgc                              >  1:643619/1‑151 (MQ=255)                                                                                                                      aTTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTgcgc                            <  1:617238/151‑1 (MQ=255)                                                                                                                      aTTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTgc                              <  1:635251/149‑1 (MQ=255)                                                                                                                      aTTAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGGATTGCACCAACAGTgc                              >  2:474072/1‑149 (MQ=255)                                                                                                                        tAACGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGcgcc                           >  2:52921/1‑150 (MQ=255)                                                                                                                          aCGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCt                        >  2:20719/1‑151 (MQ=255)                                                                                                                          aCGCGAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCt                        <  1:513874/151‑1 (MQ=255)                                                                                                                            gcgAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCa                      <  1:642113/151‑1 (MQ=255)                                                                                                                              gAAGGGTAAACCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCAtt                    <  1:208931/151‑1 (MQ=255)                                                                                                                                     aaaCCGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGa             <  1:404841/151‑1 (MQ=255)                                                                                                                                        ccGTACATCCGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACaa          <  2:291452/151‑1 (MQ=255)                                                                                                                                                ccGGCACTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATg  >  2:226191/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATG  >  NC_000913/2445004‑2445297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑