Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,488,952	T→C	S73G (AGT→GGT)	yfdE ←	acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,488,952	0	T	C	100.0%	213.4 / NA	59	S73G (AGT→GGT)	yfdE	acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

CGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAA  >  NC_000913/2488806‑2489093                                                                                                                                                   |                                                                                                                                              cgGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACAccctct                                                                                                                                           <  2:301033/151‑1 (MQ=255)  gggTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCtt                                                                                                                                          <  1:381873/151‑1 (MQ=255)  gggTTGATTTCGTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGATTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCtt                                                                                                                                          <  2:606471/151‑1 (MQ=255)         tttCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATg                                                                                                                                   >  2:845239/1‑151 (MQ=255)           tCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGa                                                                                                                                  <  2:98996/150‑1 (MQ=255)           tCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGa                                                                                                                                  <  1:523170/150‑1 (MQ=255)              tGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGAtt                                                                                                                                <  1:192141/149‑1 (MQ=255)              tGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGAt                                                                                                                                 <  1:371147/148‑1 (MQ=255)                   cGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATaaa                                                                                                                          >  2:423155/1‑150 (MQ=255)                    gTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAAc                                                                                                                        <  1:236482/151‑1 (MQ=255)                     tttCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACt                                                                                                                       >  2:666367/1‑151 (MQ=255)                     tttCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACt                                                                                                                       >  2:391258/1‑151 (MQ=255)                      ttCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTg                                                                                                                      >  2:62963/1‑151 (MQ=255)                        cccATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGt                                                                                                                     <  2:293584/150‑1 (MQ=255)                               aaaCCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATagag                                                                                                              >  1:686796/1‑150 (MQ=255)                                     cAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGt                                                                                                       >  1:831598/1‑151 (MQ=255)                                      aGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGtc                                                                                                      <  1:666416/151‑1 (MQ=255)                                        tttttCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGtcc                                                                                                     >  2:678694/1‑150 (MQ=255)                                             ccATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCcac                                                                                               >  1:370695/1‑151 (MQ=255)                                              cATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCcac                                                                                               >  2:790999/1‑150 (MQ=255)                                              cATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGAAATAGAGTGACTGTCCATCcac                                                                                               >  2:745399/1‑150 (MQ=255)                                                ttGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACAt                                                                                             >  2:64295/1‑150 (MQ=255)                                                    aCCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAggg                                                                                         <  1:494774/150‑1 (MQ=255)                                                                  tctcAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGt                                                                          >  1:383725/1‑151 (MQ=255)                                                                     cAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCTCACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTTCGGGTat                                                                        <  2:821537/150‑1 (MQ=255)                                                                      aGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTa                                                                         <  2:843113/148‑1 (MQ=255)                                                                          aaTACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTAtcatca                                                                   <  1:573404/150‑1 (MQ=255)                                                                          aaTACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCAc                                                                  >  1:98543/1‑151 (MQ=255)                                                                          aaTACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCAc                                                                  <  1:58345/151‑1 (MQ=255)                                                                           aTACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCAcc                                                                 >  1:286270/1‑151 (MQ=255)                                                                                   gCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAGGAACCACACCCTCTTTTCCATGATTAATAAACCTGTAATAGAGTGACTGTCCATCCACCTAGGGACCAAATGTGCGGGTTTCATCTCCATGACCcg                                                         <  2:764363/151‑1 (MQ=255)                                                                                       gTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCcggcgg                                                     >  1:835720/1‑151 (MQ=255)                                                                                       gTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCcggcg                                                      <  1:197534/150‑1 (MQ=255)                                                                                        tttGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCggcggc                                                    >  2:386590/1‑151 (MQ=255)                                                                                            aGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCaa                                                <  2:316761/151‑1 (MQ=255)                                                                                             gCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAAc                                               <  1:308387/151‑1 (MQ=255)                                                                                              cATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACt                                              <  1:391284/151‑1 (MQ=255)                                                                                                 atTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACttt                                            <  1:825094/150‑1 (MQ=255)                                                                                                  tttATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTa                                           >  2:728216/1‑150 (MQ=255)                                                                                                       aaaTATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTAc                                      <  2:840350/150‑1 (MQ=255)                                                                                                           ataCTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCtt                                  <  1:696331/150‑1 (MQ=255)                                                                                                            taCTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTgc                                >  1:465460/1‑151 (MQ=255)                                                                                                                 ttATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGccc                            <  2:831531/150‑1 (MQ=255)                                                                                                                   aTCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCata                         <  1:156316/151‑1 (MQ=255)                                                                                                                   aTCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCata                         <  1:63219/151‑1 (MQ=255)                                                                                                                   aTCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCat                          <  2:639132/150‑1 (MQ=255)                                                                                                                   aTCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCat                          >  1:47246/1‑150 (MQ=255)                                                                                                                      gTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTa                      <  2:708221/151‑1 (MQ=255)                                                                                                                        gATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTAc                     <  1:114656/150‑1 (MQ=255)                                                                                                                        gATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACa                    <  2:516876/151‑1 (MQ=255)                                                                                                                         aTCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACa                    >  2:431884/1‑150 (MQ=255)                                                                                                                           cATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACaa                   >  1:760827/1‑149 (MQ=255)                                                                                                                            aTTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTgcg                               >  1:356490/1‑136 (MQ=255)                                                                                                                            aTTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTgcg                               <  2:356464/136‑1 (MQ=255)                                                                                                                                    aaTCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGt        >  1:896064/1‑151 (MQ=255)                                                                                                                                      tCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTc      >  1:521973/1‑151 (MQ=255)                                                                                                                                      tCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTc      >  1:179700/1‑151 (MQ=255)                                                                                                                                       cAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTcc     >  1:491136/1‑151 (MQ=255)                                                                                                                                        aaGAACCACACCATCTTTGCCATGATTAATAAAACTGTAATAGAGTAACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCg    >  1:817978/1‑151 (MQ=255)                                                                                                                                            aCCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGaa  >  2:414658/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                              CGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAA  >  NC_000913/2488806‑2489093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑