Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,658	T→C	V238A (GTT→GCT)	yfeH →	putative inorganic ion transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,658	0	T	C	100.0%	208.0 / NA	59	V238A (GTT→GCT)	yfeH	putative inorganic ion transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

TGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGTTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCA  >  NC_000913/2527511‑2527801                                                                                                                                                    |                                                                                                                                                tgtCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGctct                                                                                                                                              >  2:263870/1‑151 (MQ=255)  gtCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGctct                                                                                                                                              <  2:276633/150‑1 (MQ=255)   tCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGctcttc                                                                                                                                            >  1:317672/1‑151 (MQ=255)    cgcgCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCtcttct                                                                                                                                           <  2:348864/151‑1 (MQ=255)      cgcAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTgg                                                                                                                                         >  2:85865/1‑151 (MQ=255)          aTAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTAt                                                                                                                                     <  2:497425/151‑1 (MQ=255)           tAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATc                                                                                                                                    >  1:251625/1‑151 (MQ=255)             aaaaaTGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGt                                                                                                                                  >  2:332274/1‑151 (MQ=255)                aaTGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGAt                                                                                                                               >  2:463311/1‑151 (MQ=255)                 aTGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGGTGGCTGGGGATCATTGCTGATTATCGTGGTGGTCAGCTGCGCACTTCTGGCTATCGTGagt                                                                                                                              <  2:783442/151‑3 (MQ=255)                      ttGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGt                                                                                                                         >  1:449144/1‑151 (MQ=255)                       tGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGt                                                                                                                         <  1:489318/150‑1 (MQ=255)                        gCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGtt                                                                                                                        >  1:390063/1‑150 (MQ=255)                        gCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTGTATACAGCGTTCAGCGAAGCCGTCGTTAATTGTATCTGGCATAAAGTTGGCTTGGGATAATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGt                                                                                                                         <  2:805657/149‑1 (MQ=255)                             aaCTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTc                                                                                                                  <  2:284065/151‑1 (MQ=255)                                 gACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTa                                                                                                              <  1:131160/151‑1 (MQ=255)                                      gACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGc                                                                                                          >  1:322217/1‑150 (MQ=255)                                            cATTCTGTTGGTGGTTTATACAGCGCTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGa                                                                                                   <  2:346036/151‑1 (MQ=255)                                              ttCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCCCTCTTCTAGCTATCGTGATTGTATTTAACCTCTTTATGGCACGCCg                                                                                                    <  2:463113/148‑1 (MQ=255)                                               tCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACt                                                                                                 <  2:791924/150‑1 (MQ=255)                                               tCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACt                                                                                                 <  1:750638/150‑1 (MQ=255)                                                  gTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAg                                                                                              <  1:544773/150‑1 (MQ=255)                                                        ggtTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAAt                                                                                       >  2:760925/1‑151 (MQ=255)                                                         gtTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAAt                                                                                       >  2:906050/1‑150 (MQ=255)                                                            tataCAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAg                                                                                    >  2:815059/1‑150 (MQ=255)                                                               aCAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGc                                                                                  >  2:250883/1‑149 (MQ=255)                                                                cAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGa                                                                               <  1:648377/151‑1 (MQ=255)                                                                 aGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGAt                                                                              <  1:543680/151‑1 (MQ=255)                                                                       cAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAAt                                                                         <  2:501114/150‑1 (MQ=255)                                                                         gCGAAGCCGTTGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTAc                                                                      <  1:251945/151‑1 (MQ=255)                                                                            aaGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGTTGTTTATCGTGGTGGTAAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAGGGCAGATGAAATtattat                                                                   >  1:805978/1‑151 (MQ=255)                                                                                cgtcgtTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTc                                                               >  2:134990/1‑151 (MQ=255)                                                                                 gtcgtTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGCCTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCt                                                              <  2:272150/151‑1 (MQ=255)                                                                                   cgtTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCttt                                                            <  2:178896/151‑1 (MQ=255)                                                                                      tAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTtg                                                          <  2:765723/150‑1 (MQ=255)                                                                                       aaTGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTtgtg                                                        >  1:793538/1‑151 (MQ=255)                                                                                       aaTGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTtgtg                                                        <  1:208462/151‑1 (MQ=255)                                                                                          ggTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGtt                                                     <  2:607932/151‑1 (MQ=255)                                                                                           gTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTc                                                    <  2:516936/151‑1 (MQ=255)                                                                                            tATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCg                                                   >  2:775545/1‑151 (MQ=255)                                                                                             aTCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCg                                                   <  1:775596/150‑1 (MQ=255)                                                                                              tCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGa                                                  >  2:84945/1‑150 (MQ=255)                                                                                              tCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGa                                                  >  1:444202/1‑150 (MQ=255)                                                                                                    aTAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAag                                             >  1:794026/1‑149 (MQ=255)                                                                                                             gCTGTGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCaaa                                  <  1:429470/151‑1 (MQ=255)                                                                                                                ggggATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTCCTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATgg                               <  1:203983/151‑1 (MQ=255)                                                                                                                ggggATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAAt                                 <  2:331923/149‑1 (MQ=255)                                                                                                                     tCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATcc                          >  1:479792/1‑151 (MQ=255)                                                                                                                      cATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATccc                         <  1:289987/151‑1 (MQ=255)                                                                                                                         tGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGAt                      >  2:840368/1‑151 (MQ=255)                                                                                                                          gCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATg                     <  1:450509/151‑1 (MQ=255)                                                                                                                            tGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGc                   >  1:656796/1‑151 (MQ=255)                                                                                                                              tttATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCa                  >  2:258828/1‑150 (MQ=255)                                                                                                                              tttATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCa                  >  2:128132/1‑150 (MQ=255)                                                                                                                               ttATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCaa                 >  2:52048/1‑150 (MQ=255)                                                                                                                               ttATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCa                  >  2:683222/1‑149 (MQ=255)                                                                                                                                 aTCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAAc               >  1:569321/1‑150 (MQ=255)                                                                                                                                    gtggtgGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTc           >  1:696577/1‑151 (MQ=255)                                                                                                                                      ggtggtCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCt          <  1:263896/150‑1 (MQ=255)                                                                                                                                         ggtCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTc      >  1:685122/1‑151 (MQ=255)                                                                                                                                           tCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTccc    >  2:443304/1‑151 (MQ=255)                                                                                                                                             aGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCca  <  2:449097/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                TGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGTTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCA  >  NC_000913/2527511‑2527801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑