Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,576,775	T→C	N535D (AAC→GAC)	maeB ←	malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,576,775	0	T	C	100.0%	242.3 / NA	67	N535D (AAC→GAC)	maeB	malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/38);  total (29/38)

TGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCC  >  NC_000913/2576631‑2576919                                                                                                                                                 |                                                                                                                                                 tGGCACCTGCTGTGTGAACGCCATCGCGATAACCAAAGACATTTTCCACCCCGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACACTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGa                                                                                                                                             <  2:261032/150‑1 (MQ=255)     aCCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAgc                                                                                                                                        >  1:308329/1‑151 (MQ=255)         gCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAgcgc                                                                                                                                      <  1:9475/149‑1 (MQ=255)            gtgtGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCt                                                                                                                                 >  2:267318/1‑151 (MQ=255)                  aCGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCt                                                                                                                           <  2:614687/151‑1 (MQ=255)                   cGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTg                                                                                                                          <  2:27989/151‑1 (MQ=255)                     ccATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGtt                                                                                                                        <  1:606994/151‑1 (MQ=255)                      cATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTc                                                                                                                       >  2:729491/1‑151 (MQ=255)                         cgcgATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCt                                                                                                                     <  2:210744/150‑1 (MQ=255)                                  cAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGAcgcc                                                                                                           <  2:132973/151‑1 (MQ=255)                                      gACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGa                                                                                                       <  2:803537/151‑1 (MQ=255)                                         aTTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGc                                                                                                    >  1:367551/1‑151 (MQ=255)                                              tcccctcGCTAAAATGTCCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGGTCCTGAGTGACGCCGCGACGCGTCa                                                                                                <  2:198729/144‑1 (MQ=255)                                                accacGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCAt                                                                                               <  1:2577/149‑1 (MQ=255)                                                  cacGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATc                                                                                           >  1:187486/1‑151 (MQ=255)                                                        aaaaTGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCTCGCTGATCCATGATCGCGCCGATCACTGTCTGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGa                                                                                       <  2:866551/149‑1 (MQ=255)                                                        aaaaTGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGa                                                                                       <  2:135061/149‑1 (MQ=255)                                                         aaaTGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGt                                                                                    >  1:230783/1‑151 (MQ=255)                                                            tGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAtt                                                                                 >  1:295837/1‑151 (MQ=255)                                                            tGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAtt                                                                                 >  1:843773/1‑151 (MQ=255)                                                             gTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAtt                                                                                 >  1:729584/1‑150 (MQ=255)                                                             gTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTc                                                                                >  2:289577/1‑151 (MQ=255)                                                             gTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTc                                                                                >  1:711703/1‑151 (MQ=255)                                                              ttCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCg                                                                               >  1:742888/1‑151 (MQ=255)                                                               tCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGGTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCgg                                                                              >  1:185780/1‑151 (MQ=255)                                                                cATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCgg                                                                              <  2:568548/150‑1 (MQ=255)                                                                cATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGt                                                                             >  1:190010/1‑151 (MQ=255)                                                                   gATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTcc                                                                           <  2:267599/150‑1 (MQ=255)                                                                   gATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTcc                                                                           <  2:216573/150‑1 (MQ=255)                                                                      aaTCGCCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGt                                                                        <  2:514700/150‑1 (MQ=255)                                                                      aaTCACCCACCGTACCGTAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTTGGTGACGCCGCGACGATTCATGATCTGGGAGTATTCTGTCCAGt                                                                        <  2:98367/150‑1 (MQ=255)                                                                         cacccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActc                                                                    >  2:460654/1‑151 (MQ=255)                                                                         cacccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTAct                                                                     <  2:315388/150‑1 (MQ=255)                                                                           cccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCtt                                                                  <  1:580601/151‑1 (MQ=255)                                                                            ccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGCTCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCttt                                                                 >  2:727815/1‑151 (MQ=255)                                                                            ccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCttt                                                                 >  1:484758/1‑151 (MQ=255)                                                                             caccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCttt                                                                 >  2:377241/1‑150 (MQ=255)                                                                                 gTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgc                                                            <  1:317128/151‑1 (MQ=255)                                                                                 gTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgc                                                            <  1:79107/151‑1 (MQ=255)                                                                                  tACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcg                                                           >  2:829189/1‑151 (MQ=255)                                                                                    ccGCAAATCATTGCATCGGCTTCCCCACGCTGAACCCTGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGGCGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAg                                                             <  1:483315/147‑1 (MQ=255)                                                                                    ccGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcgc                                                          >  2:191233/1‑150 (MQ=255)                                                                                    ccGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGcgcg                                                         <  1:866111/151‑1 (MQ=255)                                                                                     cGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcgg                                                        <  2:77527/151‑1 (MQ=255)                                                                                      gCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcgga                                                       <  2:641880/151‑1 (MQ=255)                                                                                       cAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggat                                                      <  2:472269/151‑1 (MQ=255)                                                                                       cAAATCATTGAATCGGCTTCCCCACTCTGAACCATGATCGCGCCGAACACTGTCGGGGCACTGATCAGCGCCCGCGACAACTGTTCCTGAGTGACGCCGCAACCCTTCATGAACTGGAAGGATTCCGTTCAGTACTCTTTAAAGCCcgga                                                       >  2:879329/1‑150 (MQ=255)                                                                                         aaTCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcg                                                    >  2:350675/1‑151 (MQ=255)                                                                                         aaTCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcg                                                    <  2:409225/151‑1 (MQ=255)                                                                                         aaTCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcg                                                    <  2:659172/151‑1 (MQ=255)                                                                                          aTCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCGTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcgg                                                   <  2:613705/151‑1 (MQ=255)                                                                                           tCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcgg                                                   >  1:32022/1‑150 (MQ=255)                                                                                             aTTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcggat                                                 >  1:686548/1‑150 (MQ=255)                                                                                                gCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGt                                             <  2:473921/151‑1 (MQ=255)                                                                                                        ttCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACg                                     >  1:196267/1‑151 (MQ=255)                                                                                                          ccccACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACg                                     <  2:187320/149‑1 (MQ=255)                                                                                                             cACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAtctt                                <  2:198462/151‑2 (MQ=255)                                                                                                             cACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAtctc                                <  2:431510/151‑1 (MQ=255)                                                                                                              aCGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAtctc                                >  2:70896/1‑150 (MQ=255)                                                                                                                gCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCaa                              >  1:15558/1‑150 (MQ=255)                                                                                                                   gAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCaaaa                            <  1:544558/149‑1 (MQ=255)                                                                                                                   gAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATc                          <  2:857201/151‑1 (MQ=255)                                                                                                                             cgcgCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCt                >  1:245240/1‑151 (MQ=255)                                                                                                                                      cACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTg        <  2:308303/150‑1 (MQ=255)                                                                                                                                      cACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGc       <  1:168393/151‑1 (MQ=255)                                                                                                                                        cTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCa      >  2:351726/1‑150 (MQ=255)                                                                                                                                            cGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGcc  <  1:588368/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCC  >  NC_000913/2576631‑2576919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑