Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	359,143	T→C	H115H (CAT→CAC)	cynT →	carbonic anhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	359,143	0	T	C	100.0%	168.5 / NA	45	H115H (CAT→CAC)	cynT	carbonic anhydrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/13);  total (32/13)

GTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAC  >  NC_000913/358997‑359283                                                                                                                                                   |                                                                                                                                             gTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgc                                                                                                                                          >  1:402176/1‑151 (MQ=255) gTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgc                                                                                                                                          >  2:703314/1‑151 (MQ=255)     cTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgcctgc                                                                                                                                      >  1:669884/1‑151 (MQ=255)        ccTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGt                                                                                                                                   >  1:180245/1‑151 (MQ=255)        ccTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGt                                                                                                                                   >  2:439277/1‑151 (MQ=255)            cGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGTTGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTcc                                                                                                                               >  1:60577/1‑151 (MQ=255)            cGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTcc                                                                                                                               >  2:129553/1‑151 (MQ=255)            cGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTcc                                                                                                                               >  2:577606/1‑151 (MQ=255)                 cGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTg                                                                                                                          >  2:605370/1‑151 (MQ=255)                   gAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGc                                                                                                                        >  1:105243/1‑151 (MQ=255)                        cGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGt                                                                                                                   >  1:620970/1‑151 (MQ=255)                            ggCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATg                                                                                                               >  2:793266/1‑151 (MQ=255)                                  tCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGAtt                                                                                                         >  1:142775/1‑151 (MQ=255)                                             ggAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGcgt                                                                                               <  1:269988/150‑1 (MQ=255)                                                 tATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGtt                                                                                           >  1:34160/1‑150 (MQ=255)                                                   tGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTaa                                                                                         >  1:399368/1‑150 (MQ=255)                                                   tGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAAt                                                                                        >  2:86649/1‑151 (MQ=255)                                                    gCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATg                                                                                       >  2:287100/1‑151 (MQ=255)                                                      cGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATg                                                                                       >  2:537181/1‑149 (MQ=255)                                                             gcgcTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGcc                                                                              >  1:24800/1‑151 (MQ=255)                                                                cTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGc                                                                           >  1:786736/1‑151 (MQ=255)                                                                   cGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCAt                                                                         <  1:111720/150‑1 (MQ=255)                                                                     ggTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCAATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCGCTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTcc                                                                      <  1:846437/151‑1 (MQ=255)                                                                                 tgtgATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTc                                                           >  1:621912/1‑150 (MQ=255)                                                                                  gtgATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCaa                                                         <  2:19064/151‑1 (MQ=255)                                                                                        tgtgGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCt                                                    <  1:628442/150‑1 (MQ=255)                                                                                               aTTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGAt                                            >  1:556982/1‑151 (MQ=255)                                                                                               aTTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGCCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGAt                                            >  2:834838/1‑151 (MQ=255)                                                                                                   cAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGt                                         >  1:794703/1‑150 (MQ=255)                                                                                                       tgtgGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGACTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTg                                    >  1:336704/1‑151 (MQ=255)                                                                                                        gtgGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTg                                    >  1:406075/1‑150 (MQ=255)                                                                                                                aTGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACCGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCa                           <  2:849061/151‑1 (MQ=255)                                                                                                                  gACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCAtt                         >  1:712355/1‑151 (MQ=255)                                                                                                                    ccGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGc                       >  1:361191/1‑151 (MQ=255)                                                                                                                         aTTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCa                    <  1:712624/149‑1 (MQ=255)                                                                                                                         aTTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGt                  <  1:371559/151‑1 (MQ=255)                                                                                                                            gCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTgg               <  1:101912/151‑1 (MQ=255)                                                                                                                              cAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCt             <  2:24778/151‑1 (MQ=255)                                                                                                                                     cAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGc      >  1:464281/1‑151 (MQ=255)                                                                                                                                      aGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCa     <  1:847066/151‑1 (MQ=255)                                                                                                                                       gTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCaa    <  2:4052/151‑1 (MQ=255)                                                                                                                                       gTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCa     >  1:385964/1‑150 (MQ=255)                                                                                                                                        tGCATGGACCACATGCATGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCaa    >  2:560925/1‑150 (MQ=255)                                                                                                                                         gCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAc  >  2:423821/1‑151 (MQ=255)                                                                                                                                         gCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAc  <  2:759188/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                             GTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAC  >  NC_000913/358997‑359283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑