Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,707,777	C→T	V9I (GTC→ATC)	rseC ←	SoxR iron‑sulfur cluster reduction factor component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,707,777	0	C	T	100.0%	198.2 / NA	51	V9I (GTC→ATC)	rseC	SoxR iron‑sulfur cluster reduction factor component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/29);  total (22/29)

GCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCC  >  NC_000913/2707630‑2707917                                                                                                                                                    |                                                                                                                                             gCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAgatgac                                                                                                                                           <  2:327585/151‑1 (MQ=255)    catcaCAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGatgacg                                                                                                                                          <  2:279299/149‑1 (MQ=255)     atcaCAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGt                                                                                                                                        >  1:867942/1‑150 (MQ=255)       cacaGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGc                                                                                                                                     <  1:437120/151‑1 (MQ=255)        acaGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGcc                                                                                                                                    <  2:67464/151‑1 (MQ=255)            ggTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCAct                                                                                                                                <  2:722676/151‑1 (MQ=255)               aCGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCtt                                                                                                                             <  2:779804/151‑1 (MQ=255)                cGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCttt                                                                                                                            <  2:740727/151‑1 (MQ=255)                 gACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTg                                                                                                                           <  2:858349/151‑1 (MQ=255)                  aCAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCTGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGCTGACGGTAGCCCACTCTTTg                                                                                                                           >  2:174814/1‑150 (MQ=255)                  aCAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTg                                                                                                                           >  2:107887/1‑150 (MQ=255)                     aTGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCTCTCTTTGATCa                                                                                                                       <  1:522975/151‑1 (MQ=255)                      tGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCAt                                                                                                                      >  1:866133/1‑151 (MQ=255)                          aTGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTgcg                                                                                                                  <  2:761712/151‑1 (MQ=255)                            gCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCt                                                                                                                <  2:797906/151‑1 (MQ=255)                               tGGTTTGCGGGCCAAGATTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGcc                                                                                                             >  2:661724/1‑151 (MQ=255)                                     gCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGaa                                                                                                        <  1:568857/150‑1 (MQ=255)                                             aGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATAtt                                                                                               >  1:88670/1‑151 (MQ=255)                                               tttatttaACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATtct                                                                                             >  1:230295/1‑151 (MQ=255)                                                  atttaACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCgg                                                                                          <  2:230563/151‑1 (MQ=255)                                                    ttaACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGc                                                                                         >  2:551759/1‑150 (MQ=255)                                                      aaCACGCGGCTACCGCAACCGGTGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAAt                                                                                      <  1:87103/151‑1 (MQ=255)                                                      aaCACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAAt                                                                                      <  1:213464/151‑1 (MQ=255)                                                            cgGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGtt                                                                                 >  1:834944/1‑150 (MQ=255)                                                            cgGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGtt                                                                                 <  2:834877/150‑1 (MQ=255)                                                             ggCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTc                                                                               >  2:238238/1‑151 (MQ=255)                                                                 aCCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGc                                                                             <  1:612890/149‑1 (MQ=255)                                                                  ccGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCg                                                                           <  2:442468/150‑1 (MQ=255)                                                                    gCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGttt                                                                        >  1:257659/1‑151 (MQ=255)                                                                    gCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGttt                                                                        <  2:421325/151‑1 (MQ=255)                                                                     cAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTg                                                                       >  2:495908/1‑151 (MQ=255)                                                                      aaCCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTgc                                                                      >  2:768447/1‑151 (MQ=255)                                                                           gcgcGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGcggcgg                                                                 <  1:421994/151‑1 (MQ=255)                                                                              cgTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCggcggc                                                                >  1:320512/1‑149 (MQ=255)                                                                               gTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGt                                                             <  2:786484/151‑1 (MQ=255)                                                                               gTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGt                                                             >  1:539355/1‑151 (MQ=255)                                                                               gTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGt                                                             <  1:439959/151‑1 (MQ=255)                                                                                tGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGtt                                                            >  1:394776/1‑151 (MQ=255)                                                                                 gAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTc                                                           <  1:469937/151‑1 (MQ=255)                                                                                  aaGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGAGTTTCGCCGTTTGCGGCGGCAGTTCa                                                          <  1:173187/151‑1 (MQ=255)                                                                                      gcAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCg                                                       >  2:175800/1‑150 (MQ=255)                                                                                         gctgctGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACa                                                    >  2:831005/1‑150 (MQ=255)                                                                                                          tAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGtt                                   <  2:623348/150‑1 (MQ=255)                                                                                                            aCATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATc                                >  1:107014/1‑151 (MQ=255)                                                                                                                cacaACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGt                            <  2:714879/151‑1 (MQ=255)                                                                                                                cacaACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGt                            <  1:813039/151‑1 (MQ=255)                                                                                                                       gACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCtt                      <  1:873483/150‑1 (MQ=255)                                                                                                                             cgcCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTg                >  2:663186/1‑150 (MQ=255)                                                                                                                             cgcCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTg                >  1:205720/1‑150 (MQ=255)                                                                                                                               ccTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACg             <  2:347685/151‑1 (MQ=255)                                                                                                                                  gCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCACTGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTg                >  2:542920/1‑145 (MQ=255)                                                                                                                                           gCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTcc  <  1:182540/150‑1 (MQ=255)                                                                                                                                           gCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTcc  <  2:88648/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             GCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCC  >  NC_000913/2707630‑2707917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑