Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,712,690 A→G C25C (TGT→TGC trmN ← tRNA1(Val) (adenine(37)‑N6)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,712,6900AG98.4% 206.1 / ‑3.8 62C25C (TGT→TGCtrmNtRNA1(Val) (adenine(37)‑N6)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (24/37);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

CAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCGAGAAGCGATATCCATAC  >  NC_000913/2712548‑2712836
                                                                                                                                              |                                                                                                                                                  
caatcaTCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATc                                                                                                                                            <  1:620049/151‑1 (MQ=255)
caatcaTCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATc                                                                                                                                            <  1:322826/151‑1 (MQ=255)
   tcatcaCGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTg                                                                                                                                         >  1:650140/1‑151 (MQ=255)
    catcaCGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTg                                                                                                                                         >  2:304294/1‑150 (MQ=255)
     atcaCGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAg                                                                                                                                       <  1:429011/151‑1 (MQ=255)
      tcaCGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGc                                                                                                                                      >  1:739738/1‑151 (MQ=255)
      tcaCGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGc                                                                                                                                      >  1:893981/1‑151 (MQ=255)
       caCGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGc                                                                                                                                      <  2:157064/150‑1 (MQ=255)
       caCGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGc                                                                                                                                      <  1:465309/150‑1 (MQ=255)
         cGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGcaac                                                                                                                                   <  2:558904/151‑1 (MQ=255)
           cTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGcaaca                                                                                                                                  <  2:71590/150‑1 (MQ=255)
             gTCATCGGTTCGCTGCGCCAGAATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACaaaa                                                                                                                               <  2:896674/151‑1 (MQ=255)
                aTCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAAc                                                                                                                            <  1:332700/151‑1 (MQ=255)
                     ttCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGttt                                                                                                                       >  2:339237/1‑151 (MQ=255)
                       cGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTa                                                                                                                      >  2:196784/1‑150 (MQ=255)
                           gcgcCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAg                                                                                                                  <  1:894423/150‑1 (MQ=255)
                            cgcCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAg                                                                                                                  >  1:600165/1‑149 (MQ=255)
                            cgcCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTa                                                                                                                <  1:88683/151‑1 (MQ=255)
                                  cATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCa                                                                                                          <  2:893910/151‑1 (MQ=255)
                                  cATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCa                                                                                                          <  2:811123/151‑1 (MQ=255)
                                      aaTGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTac                                                                                                      <  2:385021/151‑1 (MQ=255)
                                         gCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTacgac                                                                                                   >  1:7537/1‑151 (MQ=255)
                                         gCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTacgac                                                                                                   <  2:369511/151‑1 (MQ=255)
                                          ccAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTacgacg                                                                                                  >  2:821338/1‑151 (MQ=255)
                                           cAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTacgacg                                                                                                  >  2:21017/1‑150 (MQ=255)
                                            aGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGaa                                                                                                <  1:553645/151‑1 (MQ=255)
                                             gCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTAcgacga                                                                                                 >  1:468290/1‑149 (MQ=255)
                                             gCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAg                                                                                               <  1:233920/151‑1 (MQ=255)
                                                 cccGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACg                                                                                           <  2:822918/151‑1 (MQ=255)
                                                  ccGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACgg                                                                                          >  1:601173/1‑151 (MQ=255)
                                                    gCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGAt                                                                                        <  2:265420/151‑1 (MQ=255)
                                                       aCCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGt                                                                                      <  1:177763/150‑1 (MQ=255)
                                                        cccGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGt                                                                                      >  2:604116/1‑149 (MQ=255)
                                                                gATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGAc                                                                            >  2:486486/1‑151 (MQ=255)
                                                                   atCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACAt                                                                          <  1:818533/150‑1 (MQ=255)
                                                                   atCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACAt                                                                          <  1:677910/150‑1 (MQ=255)
                                                                   atCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACAt                                                                          <  1:463447/150‑1 (MQ=255)
                                                                   atCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATg                                                                         <  1:809257/151‑1 (MQ=255)
                                                                     cAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGa                                                                        >  2:897758/1‑150 (MQ=255)
                                                                      aaGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCGCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGaaa                                                                      <  2:878394/151‑1 (MQ=255)
                                                                       aGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGATATGaaaa                                                                     <  2:674376/151‑1 (MQ=255)
                                                                         gCAACGTTTTCTCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAACAt                                                                    >  2:531258/1‑150 (MQ=255)
                                                                              gTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAAcc                                                              <  1:757388/151‑1 (MQ=255)
                                                                               ttttACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCt                                                             >  1:478098/1‑151 (MQ=255)
                                                                                tttACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCt                                                             <  1:489197/150‑1 (MQ=255)
                                                                                           cACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAAc                                                 >  1:129724/1‑151 (MQ=255)
                                                                                            aCCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCAGCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACg                                                <  1:423435/151‑1 (MQ=255)
                                                                                              cGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGc                                              <  2:236241/151‑1 (MQ=255)
                                                                                               ggTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCg                                             <  1:23507/151‑1 (MQ=255)
                                                                                               ggTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCg                                             >  1:419526/1‑151 (MQ=255)
                                                                                                  gCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATa                                          <  2:907097/151‑1 (MQ=255)
                                                                                                      aTGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCAc                                      >  1:57025/1‑151 (MQ=255)
                                                                                                          gcCCAATAAAATACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCggg                                  >  2:334174/1‑151 (MQ=255)
                                                                                                                   aaTACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACaa                         <  2:871578/151‑1 (MQ=255)
                                                                                                                   aaTACCATCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACaa                         <  1:111454/151‑1 (MQ=255)
                                                                                                                         aTCCGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCg                   <  1:636641/151‑1 (MQ=255)
                                                                                                                           ccGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCg                   >  2:864112/1‑149 (MQ=255)
                                                                                                                            cGTTCCCACTTTCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCgaga                <  1:251/151‑1 (MQ=255)
                                                                                                                                       tCATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCGAGAAGCGATATcc      >  2:702563/1‑150 (MQ=255)
                                                                                                                                        cATCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCGAGAAGCGATATCCAt    >  2:278262/1‑151 (MQ=255)
                                                                                                                                         aTCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCggg                                  <  2:830610/120‑1 (MQ=255)
                                                                                                                                         aTCGCGCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCggg                                  >  1:830677/1‑120 (MQ=255)
                                                                                                                                           cgcgCAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCGAGAAGCGATATCCATAc  >  1:480589/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACCCGAGAAGCGATATCCATAC  >  NC_000913/2712548‑2712836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: