Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,714,456	T→C	S289G (AGT→GGT)	yfiE ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,714,456	0	T	C	100.0%	203.7 / NA	57	S289G (AGT→GGT)	yfiE	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/24);  total (33/24)

CGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCA  >  NC_000913/2714311‑2714601                                                                                                                                                  |                                                                                                                                                  cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCtt                                                                                                                                              >  1:715306/1‑151 (MQ=255)   tGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGGGGGATAAGGGGTGCAGGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGGCAAGTTAAATGCCGGGTTTAAGCCCGACATTATTTTTATCCAGCCACAAAACCCTCtt                                                                                                                                              <  2:166881/149‑1 (MQ=37)      cTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAac                                                                                                                                          >  2:748533/1‑150 (MQ=255)               tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAAt                                                                                                                                 >  2:268671/1‑150 (MQ=255)                atatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATa                                                                                                                                >  2:630140/1‑150 (MQ=255)                  atTGAATTTGCACGATCTTGAAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATaaaa                                                                                                                             <  2:765260/151‑1 (MQ=255)                   ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATaaa                                                                                                                              >  1:126405/1‑149 (MQ=255)                     gAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAgt                                                                                                                           <  1:110750/150‑1 (MQ=255)                      aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAgtgt                                                                                                                         >  2:270122/1‑151 (MQ=255)                      aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAgtgt                                                                                                                         >  1:799241/1‑151 (MQ=255)                       aTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGtgtg                                                                                                                        <  2:348449/151‑1 (MQ=255)                          tGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCAt                                                                                                                     >  1:273359/1‑151 (MQ=255)                           gCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCa                                                                                                                      <  1:434063/149‑1 (MQ=255)                           gCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCa                                                                                                                      <  2:739511/149‑1 (MQ=255)                            cACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCg                                                                                                                   <  1:276565/151‑1 (MQ=255)                               gATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCg                                                                                                                >  2:545178/1‑151 (MQ=255)                                aTCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCgg                                                                                                               >  2:586091/1‑151 (MQ=255)                                          ccGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCt                                                                                                     <  2:303727/151‑1 (MQ=255)                                                   gCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGc                                                                                             >  2:297219/1‑150 (MQ=255)                                                     gTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatg                                                                                          >  1:139653/1‑151 (MQ=255)                                                       tCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatgat                                                                                        >  2:843582/1‑151 (MQ=255)                                                        cACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatga                                                                                         <  1:56029/149‑1 (MQ=255)                                                                aTCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGcac                                                                                >  1:219046/1‑150 (MQ=255)                                                                    ggCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGc                                                                           <  1:752085/151‑1 (MQ=255)                                                                      cATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGGTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCg                                                                         >  1:726201/1‑151 (MQ=255)                                                                          aaTAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTaa                                                                      <  1:907587/150‑1 (MQ=255)                                                                          aaTAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAAt                                                                     <  1:554032/151‑1 (MQ=255)                                                                              aaGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTc                                                                 >  1:429410/1‑151 (MQ=255)                                                                                gcgcACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTg                                                               >  2:777046/1‑151 (MQ=255)                                                                                  gcACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCg                                                             <  1:317106/151‑1 (MQ=255)                                                                                      tttGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTg                                                         >  1:708298/1‑151 (MQ=255)                                                                                      tttGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTg                                                         >  2:195955/1‑151 (MQ=255)                                                                                        tGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGtt                                                       >  1:501430/1‑151 (MQ=255)                                                                                         gTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTTAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGtt                                                       <  1:490548/150‑1 (MQ=255)                                                                                            gACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGcc                                                   >  2:727587/1‑151 (MQ=255)                                                                                              cAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCa                                                  >  2:310344/1‑150 (MQ=255)                                                                                                        gCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaattc                                       >  2:491883/1‑151 (MQ=255)                                                                                                        gCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaattc                                       >  2:612027/1‑151 (MQ=255)                                                                                                        gCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaatt                                        <  1:105594/150‑1 (MQ=255)                                                                                                         ccGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaattca                                      <  1:837105/151‑1 (MQ=255)                                                                                                               ttAATCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCaattaat                                >  1:544924/1‑151 (MQ=255)                                                                                                                 aaTCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCaattaatt                               <  1:624784/150‑1 (MQ=255)                                                                                                                 aaTCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCaattaatt                               <  2:811948/150‑1 (MQ=255)                                                                                                                 aaTCCCGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATtc                              <  1:257036/151‑1 (MQ=255)                                                                                                                    cccGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTcc                           >  2:118359/1‑151 (MQ=255)                                                                                                                     ccGACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCg                          <  1:245666/151‑1 (MQ=255)                                                                                                                       gACATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGc                         >  1:416893/1‑150 (MQ=255)                                                                                                                        aCATTATTTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCAc                       <  2:196586/151‑1 (MQ=255)                                                                                                                           ttattTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTcc                    >  1:550189/1‑151 (MQ=255)                                                                                                                            tattTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTcc                    >  2:63283/1‑150 (MQ=255)                                                                                                                             attTTTATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCa                   >  1:423644/1‑150 (MQ=255)                                                                                                                                tttATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAActc               >  1:433607/1‑151 (MQ=255)                                                                                                                                  tATCCAGCCACAAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAActct              <  1:111923/150‑1 (MQ=255)                                                                                                                                   agccAGCCACAAAACCCTCTTCAACCCACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAGTGGTCTGCGACTGTTCGCCAAAACGCAATTCAATTAAGTCTCCGCACTCCAAc                 <  2:61319/144‑1 (MQ=255)                                                                                                                                            caAAACCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGc   >  1:695964/1‑151 (MQ=255)                                                                                                                                             aaaaCCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCa  <  1:630189/151‑1 (MQ=255)                                                                                                                                              aaaCCCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCa  >  1:706969/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATTCAATTAATTCTCCGCACTCCAACTCTTTTGCCACCGCA  >  NC_000913/2714311‑2714601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑