Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,807,897	A→G	T256A (ACC→GCC)	proX →	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,807,897	0	A	G	100.0%	191.5 / NA	53	T256A (ACC→GCC)	proX	glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/34);  total (19/34)

CACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGG  >  NC_000913/2807752‑2808044                                                                                                                                                  |                                                                                                                                                    cacCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATg                                                                                                                                                <  1:531233/151‑1 (MQ=255)   ccTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGc                                                                                                                                               <  2:563248/150‑1 (MQ=255)          gCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGtt                                                                                                                                       >  1:857546/1‑151 (MQ=255)          gCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGtt                                                                                                                                       >  2:866613/1‑151 (MQ=255)                  gggTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCcaac                                                                                                                                 <  1:557883/149‑1 (MQ=255)                  gggTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCcaac                                                                                                                                 <  1:436304/149‑1 (MQ=255)                        gTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTg                                                                                                                         <  1:399425/151‑1 (MQ=255)                        gTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTg                                                                                                                         <  2:47961/151‑1 (MQ=255)                          aaCGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTggg                                                                                                                       <  1:710219/151‑1 (MQ=255)                          aaCGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTgg                                                                                                                        <  1:663870/150‑1 (MQ=255)                                cTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCg                                                                                                                    >  1:331575/1‑148 (MQ=255)                                  gAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGaaa                                                                                                               <  1:787012/151‑1 (MQ=255)                                  gAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGaaa                                                                                                               <  1:154778/151‑1 (MQ=255)                                  gAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGaa                                                                                                                <  2:555676/150‑1 (MQ=255)                                       cGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAAccc                                                                                                          <  2:571805/151‑1 (MQ=255)                                          gCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGgc                                                                                                       <  1:251121/151‑1 (MQ=255)                                            aaaGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAACCCCGgcag                                                                                                     <  2:315795/151‑1 (MQ=255)                                            aaaGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGgcag                                                                                                     <  1:558584/151‑1 (MQ=255)                                                    cgtcTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGaa                                                                                              <  2:610733/150‑1 (MQ=255)                                                      tcTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACt                                                                                           >  2:386448/1‑151 (MQ=255)                                                         ggTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACt                                                                                           <  1:834737/148‑1 (MQ=255)                                                               aGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCAt                                                                                  >  2:892222/1‑151 (MQ=255)                                                                ggTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCAtt                                                                                 <  1:84666/151‑1 (MQ=255)                                                                  tGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTAt                                                                               >  2:474880/1‑151 (MQ=255)                                                                   gCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTAt                                                                               <  2:680007/150‑1 (MQ=255)                                                                    ccGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAATCTGTTTGCCATTAt                                                                               <  2:520733/149‑1 (MQ=255)                                                                     cGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGc                                                                             >  2:107825/1‑150 (MQ=255)                                                                      gTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAg                                                                           <  2:491154/151‑1 (MQ=255)                                                                       ttCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGt                                                                          >  1:54218/1‑151 (MQ=255)                                                                             gCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAg                                                                    <  2:446392/151‑1 (MQ=255)                                                                              cACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAg                                                                    <  1:309427/150‑1 (MQ=255)                                                                               aCTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAg                                                                    >  2:826397/1‑149 (MQ=255)                                                                                  gCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGTAGTTGCCAGTGGCa                                                               <  1:776390/151‑1 (MQ=255)                                                                                      ggCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGata                                                           <  2:294772/151‑1 (MQ=255)                                                                                               aaCGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGccc                                                  >  1:775500/1‑151 (MQ=255)                                                                                                 cGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGccc                                                  >  2:373979/1‑149 (MQ=255)                                                                                                    cGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAAc                                             <  2:49644/151‑1 (MQ=255)                                                                                                      aTACCAACCTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGc                                           >  1:850989/1‑151 (MQ=255)                                                                                                        aCCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGcc                                          >  2:608078/1‑150 (MQ=255)                                                                                                          cAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCAtt                                       <  1:574135/151‑1 (MQ=255)                                                                                                           aaaCTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCAtt                                       <  1:113408/150‑1 (MQ=255)                                                                                                            aaCTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTAt                                     >  1:531382/1‑151 (MQ=255)                                                                                                            aaCTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTAt                                     >  2:108759/1‑151 (MQ=255)                                                                                                            aaCTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTAt                                     >  2:290165/1‑151 (MQ=255)                                                                                                                 ccGAATGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATg                                >  2:426166/1‑151 (MQ=255)                                                                                                                     aTGGTGCGAATTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACgg                            <  1:466299/151‑1 (MQ=255)                                                                                                                              aTTATGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTc                   >  2:282629/1‑151 (MQ=255)                                                                                                                                 aTGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAg                 <  2:115113/150‑1 (MQ=255)                                                                                                                                 aTGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGa                <  1:642762/151‑1 (MQ=255)                                                                                                                                  tGGCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGa                >  2:493969/1‑150 (MQ=255)                                                                                                                                   ggCTTCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACCGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGaa               <  2:296095/150‑1 (MQ=255)                                                                                                                                       tCCCGGTCAGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGc            <  2:388326/149‑1 (MQ=255)                                                                                                                                               aGCGCCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAgg  <  2:63078/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CACCTGGACGCCGTACTGGGTGAGTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGG  >  NC_000913/2807752‑2808044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑