Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	373,704	G→A	V262M (GTG→ATG)	mhpF →	acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	373,704	0	G	A	100.0%	162.1 / NA	44	V262M (GTG→ATG)	mhpF	acetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/28);  total (16/28)

TTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCG  >  NC_000913/373557‑373847                                                                                                                                                    |                                                                                                                                                ttGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCCGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGGCAATTTACCGGGCATgg                                                                                                                                              >  1:646485/1‑151 (MQ=255)   gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATggg                                                                                                                                             <  2:266381/150‑1 (MQ=255)     gCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCa                                                                                                                                          <  1:748067/151‑1 (MQ=255)         cgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATtc                                                                                                                                      <  1:8910/151‑1 (MQ=255)           aaGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGGTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCCGGCATGGGGCAATtct                                                                                                                                     >  1:36979/1‑150 (MQ=255)            aGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTcc                                                                                                                                   <  2:146515/151‑1 (MQ=255)                 cacaAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACt                                                                                                                              <  1:677271/151‑1 (MQ=255)                 cacaAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACt                                                                                                                              >  1:558294/1‑151 (MQ=255)                  acaAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTg                                                                                                                             <  2:38272/151‑1 (MQ=255)                        tgatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGGATGGGGCAATTCTCCGGGCTGaaaa                                                                                                                         >  2:625706/1‑149 (MQ=255)                         gatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAg                                                                                                                      <  1:144041/151‑1 (MQ=255)                            atCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCg                                                                                                                    <  2:886826/150‑1 (MQ=255)                            atCGAAGCCTCAATCAATGAAATGGCTGAAGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAg                                                                                                                      <  1:254036/148‑1 (MQ=255)                             tCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGGACCGGGTTATTGCCTGAAACAGCGCGTGCAGGTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCggg                                                                                                                  >  2:565002/1‑150 (MQ=255)                               gAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTct                                                                                                                <  2:646436/151‑1 (MQ=255)                                aaGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTctg                                                                                                               <  1:403913/151‑1 (MQ=255)                                  gCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGGGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGGctgg                                                                                                              >  2:771301/1‑150 (MQ=255)                                      caatcaatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGa                                                                                                         <  1:279845/151‑1 (MQ=255)                                          caatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGt                                                                                                      <  1:728898/150‑1 (MQ=255)                                                 aTGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAGGgcg                                                                                              <  2:244288/151‑1 (MQ=255)                                                 aTGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcg                                                                                              <  1:338384/151‑1 (MQ=255)                                                   ggCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgc                                                                                             <  1:557113/150‑1 (MQ=255)                                                       gAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgcagc                                                                                          <  2:638192/149‑1 (MQ=255)                                                            ggTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTAt                                                                                   <  2:573923/151‑1 (MQ=255)                                                             gTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATc                                                                                  >  1:436035/1‑151 (MQ=255)                                                               gCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctg                                                                                <  1:754281/151‑1 (MQ=255)                                                                   gCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctgcctg                                                                            <  1:725526/151‑1 (MQ=255)                                                                   gCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctgcctg                                                                            <  2:863314/151‑1 (MQ=255)                                                                      tACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctgcctgc                                                                           <  1:53545/149‑1 (MQ=255)                                                                           aCCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCg                                                                    >  2:277325/1‑151 (MQ=255)                                                                            ccGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCg                                                                    <  1:589616/150‑1 (MQ=255)                                                                              gggTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGc                                                                 <  1:735355/151‑1 (MQ=255)                                                                                       ccTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGAGAAACCGGTCAATTTACCGGGCATGAGGCAATTCTCCGGACTGAAAACAGCGGGCTGGCTGGAAGGCGAATGCGCAGCGCATTATCGGCCTGCCTATGCGGGCAACCTcagc                                                        >  2:433224/1‑148 (MQ=255)                                                                                           aaaCAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACAtt                                                     >  1:15311/1‑150 (MQ=255)                                                                                              cAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATg                                                  >  2:627358/1‑150 (MQ=255)                                                                                              cAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATg                                                  >  2:204943/1‑150 (MQ=255)                                                                                                        aGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTgc                                       <  1:627407/151‑1 (MQ=255)                                                                                                        aGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTgc                                       <  2:885267/151‑1 (MQ=255)                                                                                                          tttGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAGAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTgcgc                                     >  2:316177/1‑151 (MQ=255)                                                                                                                gTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCg                                >  2:253419/1‑150 (MQ=255)                                                                                                                                     gTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGt          <  1:597820/151‑1 (MQ=255)                                                                                                                                         aTTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACt      >  1:564019/1‑151 (MQ=255)                                                                                                                                            tACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTgg    <  1:345499/150‑1 (MQ=255)                                                                                                                                              ccGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcg  >  2:900787/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                TTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCG  >  NC_000913/373557‑373847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑