Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,185	T→C	L139P (CTC→CCC)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,185	0	T	C	100.0%	208.8 / NA	59	L139P (CTC→CCC)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/38);  total (21/38)

CGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAT  >  NC_000913/2811037‑2811327                                                                                                                                                     |                                                                                                                                               cgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAAccct                                                                                                                                              >  2:171016/1‑151 (MQ=255)  gTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTg                                                                                                                                             >  2:655978/1‑151 (MQ=255)       gCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCcg                                                                                                                                        <  2:567964/151‑1 (MQ=255)        ccGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCcgc                                                                                                                                       <  1:849570/151‑1 (MQ=255)            tGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCt                                                                                                                                    >  1:156032/1‑150 (MQ=255)             gAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTc                                                                                                                                   <  1:420334/150‑1 (MQ=255)             gAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCa                                                                                                                                  <  1:893735/151‑1 (MQ=255)                  ggAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGcac                                                                                                                              <  1:308205/150‑1 (MQ=255)                    aaaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAACTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTACGCGCTCAGcacagc                                                                                                                           >  2:488474/1‑149 (MQ=255)                     aaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCacaac                                                                                                                           >  2:711354/1‑150 (MQ=255)                     aaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCacaac                                                                                                                           >  2:283706/1‑150 (MQ=255)                      aaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAg                                                                                                                         <  2:864517/151‑1 (MQ=255)                       aaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCacaac                                                                                                                           >  1:165295/1‑148 (MQ=255)                       aaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCacaac                                                                                                                           >  2:260660/1‑148 (MQ=255)                         cgcgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTGTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaa                                                                                                                      <  2:900929/151‑1 (MQ=255)                           cgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaaa                                                                                                                     <  2:542596/150‑1 (MQ=255)                             gTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGa                                                                                                                  <  1:361822/151‑1 (MQ=255)                             gTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGa                                                                                                                  <  2:903053/151‑1 (MQ=255)                               tGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGAt                                                                                                                 <  2:71922/150‑1 (MQ=255)                                ggATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCa                                                                                                               <  2:209315/151‑1 (MQ=255)                                    cGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTc                                                                                                           <  2:120834/151‑1 (MQ=255)                                    cGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTc                                                                                                           <  2:289045/151‑1 (MQ=255)                                     gAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCg                                                                                                          >  2:198686/1‑151 (MQ=255)                                      aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGa                                                                                                         <  2:69087/151‑1 (MQ=255)                                      aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGa                                                                                                         <  2:543358/151‑1 (MQ=255)                                        cgtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGc                                                                                                       <  1:510979/151‑1 (MQ=255)                                         gtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGc                                                                                                       >  1:159554/1‑150 (MQ=255)                                         gtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCa                                                                                                      <  2:355502/151‑1 (MQ=255)                                             tGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATc                                                                                                  >  1:563951/1‑151 (MQ=255)                                              gAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCa                                                                                                 <  1:856968/151‑1 (MQ=255)                                                        aCGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAAtt                                                                                       <  2:850109/151‑1 (MQ=255)                                                             cgccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGctct                                                                                  <  2:412088/151‑1 (MQ=255)                                                               ccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTcc                                                                                >  1:440439/1‑151 (MQ=255)                                                                cgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTccc                                                                               <  1:361262/151‑1 (MQ=255)                                                                    gcctgcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtct                                                                           >  2:287865/1‑151 (MQ=255)                                                                     cctgcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtctc                                                                          <  2:94259/151‑1 (MQ=255)                                                                     cctgcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtctc                                                                          <  1:167822/151‑1 (MQ=255)                                                                     cctgcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtctc                                                                          <  2:866150/151‑1 (MQ=255)                                                                              gCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCa                                                                     <  1:783323/147‑1 (MQ=255)                                                                              gCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGAt                                                                  <  1:285505/150‑1 (MQ=255)                                                                               cAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGGTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATgg                                                                >  2:570027/1‑151 (MQ=255)                                                                               cAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCa                                                                     <  1:248620/146‑1 (MQ=255)                                                                                    aaCGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACa                                                            >  2:111064/1‑150 (MQ=255)                                                                                       ggAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGAc                                                        >  1:350348/1‑151 (MQ=255)                                                                                         aaaaaGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg                                                      >  2:609266/1‑151 (MQ=255)                                                                                           aaaGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGgtg                                                    >  1:798457/1‑151 (MQ=255)                                                                                                 cACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtc                                              >  1:471406/1‑151 (MQ=255)                                                                                                 cACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtc                                              <  1:155048/151‑1 (MQ=255)                                                                                                    gAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCg                                           <  1:695736/151‑1 (MQ=255)                                                                                                       tttttGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGa                                          >  1:84145/1‑149 (MQ=255)                                                                                                             cgcgAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATg                                   >  1:280376/1‑150 (MQ=255)                                                                                                                      ttACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAAcgc                          <  2:756407/150‑1 (MQ=255)                                                                                                                        accaccGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTc                       <  2:333444/151‑1 (MQ=255)                                                                                                                         ccaccGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTc                       >  1:803051/1‑150 (MQ=255)                                                                                                                                 gcaTAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTc              <  1:326553/151‑1 (MQ=255)                                                                                                                                  caTAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCa             <  2:563901/151‑1 (MQ=255)                                                                                                                                         gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAAt        <  2:733099/149‑1 (MQ=255)                                                                                                                                         gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAAt        <  2:442754/149‑1 (MQ=255)                                                                                                                                         gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAAt        <  2:421110/149‑1 (MQ=255)                                                                                                                                             gcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAt  <  1:495096/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               CGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAT  >  NC_000913/2811037‑2811327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑