Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,577	A→G	V812A (GTT→GCT)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,577	0	A	G	100.0%	187.2 / NA	53	V812A (GTT→GCT)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

GGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCC  >  NC_000913/2819430‑2819720                                                                                                                                                    |                                                                                                                                                 ggTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcct                                                                                                                                               <  2:635829/151‑1 (MQ=255)  gTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcctt                                                                                                                                              <  1:221095/151‑1 (MQ=255)           cagcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGAc                                                                                                                                     >  1:593634/1‑151 (MQ=255)            agcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACt                                                                                                                                    >  1:841402/1‑151 (MQ=255)            agcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACt                                                                                                                                    >  2:327252/1‑151 (MQ=255)                tCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCg                                                                                                                                >  1:473514/1‑151 (MQ=255)                tCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCg                                                                                                                                >  2:761379/1‑151 (MQ=255)                  cctACCACCGCATTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTCCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATATGTTACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGtt                                                                                                                              >  2:432648/3‑151 (MQ=255)                  cGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGtt                                                                                                                              <  1:699396/151‑1 (MQ=255)                     accaccGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGcc                                                                                                                           <  1:330834/151‑1 (MQ=255)                           ggCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACg                                                                                                                     <  2:210351/151‑1 (MQ=255)                                     aTGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTc                                                                                                            <  2:723239/150‑1 (MQ=255)                                          aGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGAcccc                                                                                                      <  1:758197/151‑1 (MQ=255)                                               gTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTg                                                                                                              <  2:730504/138‑1 (MQ=255)                                               gTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTg                                                                                                              >  1:730553/1‑138 (MQ=255)                                                tCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTg                                                                                                 >  2:669270/1‑150 (MQ=255)                                                      accCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtttttt                                                                                          <  1:668984/151‑1 (MQ=255)                                                            gccgccCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATc                                                                                     <  1:177264/150‑1 (MQ=255)                                                            gccgccCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCg                                                                                    <  1:558073/151‑1 (MQ=255)                                                                 ccACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAc                                                                               >  1:594191/1‑151 (MQ=255)                                                                 ccACCTGCTGACCGACCATCCCAATCAATTCCCCTGCTTTCACACGATCTGTGACTTCCTTATATACGCCTGCAATCAGAGAAGCCTTATCTTCGTCTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCg                                                                                    <  2:894322/146‑1 (MQ=255)                                                                    ccTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CAt                                                                           >  2:282443/1‑151 (MQ=255)                                                                      tgctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTTTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATgg                                                                         >  1:263604/1‑151 (MQ=255)                                                                       gctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATg                                                                          <  1:45443/149‑1 (MQ=255)                                                                       gctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGt                                                                        <  2:220084/151‑1 (MQ=255)                                                                          gAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTa                                                                       <  2:738941/149‑1 (MQ=255)                                                                          gAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACg                                                                     >  2:329071/1‑151 (MQ=255)                                                                           aGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGc                                                                    >  1:719498/1‑151 (MQ=255)                                                                             cGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCa                                                                   >  1:220167/1‑150 (MQ=255)                                                                              gACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAAc                                                                 >  1:88792/1‑151 (MQ=255)                                                                                   tACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACAtttt                                                            >  2:260508/1‑151 (MQ=255)                                                                                    aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACAtttt                                                            <  2:393730/150‑1 (MQ=255)                                                                                    aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTc                                                           <  2:720286/151‑1 (MQ=255)                                                                                    aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTc                                                           <  2:49778/151‑1 (MQ=255)                                                                                     ccAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCCACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTc                                                           <  2:431000/150‑1 (MQ=255)                                                                                      cAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCgg                                                         <  2:494197/151‑1 (MQ=255)                                                                                       aaTCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGc                                                        <  1:351746/151‑1 (MQ=255)                                                                                              tCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAacac                                                 >  2:232812/1‑151 (MQ=255)                                                                                               ccccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACAcc                                                <  1:706940/151‑1 (MQ=255)                                                                                                 ccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGc                                              >  1:429776/1‑151 (MQ=255)                                                                                                 ccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGc                                              >  1:721993/1‑151 (MQ=255)                                                                                                    gCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTa                                            >  2:645622/1‑150 (MQ=255)                                                                                                       ttCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCt                                        >  1:168276/1‑151 (MQ=255)                                                                                                       ttCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCt                                        >  1:152988/1‑151 (MQ=255)                                                                                                            acGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTa                                   <  2:909867/151‑1 (MQ=255)                                                                                                                   gtgACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCa                               <  1:874448/148‑1 (MQ=255)                                                                                                                    tgacgggcatAGATACGCATGCAATCAGAGAAGCCTTATCTTCGACGGCCGTTGCCAGCACGATAATTGTCGGCTCCCGTTGATTTTTTAACTCGTCACCTTATGG‑ACGCACCATTGTCGGATCAACACCGCCAAGCTCGCTacc                                 >  2:778292/10‑143 (MQ=255)                                                                                                                    tgACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAAc                             >  2:114843/1‑149 (MQ=255)                                                                                                                       cGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCtt                        <  2:577647/151‑1 (MQ=255)                                                                                                                        gTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCtt                        >  1:94913/1‑150 (MQ=255)                                                                                                                         tCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTa                       >  1:96802/1‑150 (MQ=255)                                                                                                                          ccTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAac                     >  2:494751/1‑151 (MQ=255)                                                                                                                                aTACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCAtt               <  2:98272/151‑1 (MQ=255)                                                                                                                                  aCGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTaa             <  2:499583/151‑1 (MQ=255)                                                                                                                                              agagAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGcc  >  2:624335/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCC  >  NC_000913/2819430‑2819720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑