Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,837,983	T→C	L41L (TTA→TTG)	hydN ←	formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,837,983	0	T	C	100.0%	165.3 / NA	49	L41L (TTA→TTG)	hydN	formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/29);  total (20/29)

GCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGC  >  NC_000913/2837839‑2838118                                                                                                                                                 |                                                                                                                                        gCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCaaaaaa                                                                                                                                   <  2:666975/151‑1 (MQ=255) gCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCaaaaaa                                                                                                                                   <  2:645424/151‑1 (MQ=255)       ttCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTcc                                                                                                                             >  2:322787/1‑151 (MQ=255)         ccTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCgg                                                                                                                           <  1:22270/151‑1 (MQ=255)          cTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCggg                                                                                                                          >  2:874767/1‑151 (MQ=255)             cATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTc                                                                                                                       <  1:427136/151‑1 (MQ=255)                cacaTGAACAAACCCTTTATCACGGCTGATAGCCCCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACGCCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCggg                                                                                                                          <  2:535445/145‑1 (MQ=255)                cacaTGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAg                                                                                                                     <  2:647794/150‑1 (MQ=255)                 acaTGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGc                                                                                                                    <  2:508995/150‑1 (MQ=255)                     gAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGc                                                                                                               >  1:116910/1‑151 (MQ=255)                      aaCAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGc                                                                                                               >  1:887538/1‑150 (MQ=255)                       aCAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGcac                                                                                                             <  2:127430/151‑1 (MQ=255)                           aCCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGTTGCATCTTCACACTGACGGCAAACTGTAGCCGTGGAAATGTTCACACCTTTAATGACATGGAAACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGt                                                                                                          >  2:151495/1‑150 (MQ=255)                               tttATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTg                                                                                                      >  1:870952/1‑150 (MQ=255)                                   tCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGTAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGAtttt                                                                                                 >  1:553621/1‑151 (MQ=255)                                    cACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTc                                                                                                <  1:461897/151‑1 (MQ=255)                                     aCGGCTGATAGCGCCAAGCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAATTTTCCGGGGTCAGCGATGCACAGTCCTGAtt                                                                                                   <  2:145591/147‑1 (MQ=255)                                               gCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGaa                                                                                      >  2:688936/1‑150 (MQ=255)                                                cACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAAc                                                                                    <  2:456633/151‑1 (MQ=255)                                                        gggCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcacgcacg                                                                            >  2:639725/1‑151 (MQ=255)                                                          gCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATATTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCt                                                                          <  2:280341/151‑1 (MQ=255)                                                           cAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCt                                                                          <  1:495672/150‑1 (MQ=255)                                                            aGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGTTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTAc                                                                        <  2:824746/151‑1 (MQ=255)                                                              aCGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt                                                                      <  2:583051/151‑1 (MQ=255)                                                                      gcACGGTGCATCTTCACACTGCCGGCAAACCGTCGCCGTGGAAATGTTCACACCTTTAATGCCATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAACCCACGCACGCTACTTCACAGGTa                                                              <  2:381700/151‑1 (MQ=255)                                                                         cGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACgg                                                           <  1:17671/151‑1 (MQ=255)                                                                           gTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc                                                          >  1:330619/1‑150 (MQ=255)                                                                           gTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCa                                                         <  2:224524/151‑1 (MQ=255)                                                                                    cacaCTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATAc                                                 <  1:597381/150‑1 (MQ=255)                                                                                     acacTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAt                                               >  2:39902/1‑151 (MQ=255)                                                                                          gACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc                                           <  1:753992/150‑1 (MQ=255)                                                                                               cAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt                                     <  1:858353/151‑1 (MQ=255)                                                                                                   cTGTCGCCGTGGAAATGTTCACACCTTCAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc                                 >  1:37574/1‑151 (MQ=255)                                                                                                     gTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa                                >  1:272689/1‑150 (MQ=255)                                                                                                     gTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa                                <  1:618115/150‑1 (MQ=255)                                                                                                          cGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgatg                          <  2:539454/151‑1 (MQ=255)                                                                                                             ggAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaa                        >  2:457648/1‑150 (MQ=255)                                                                                                                  tGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt                  >  1:439170/1‑151 (MQ=255)                                                                                                                      cacaCCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat               <  2:617975/150‑1 (MQ=255)                                                                                                                        cacCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaa            <  2:781399/151‑1 (MQ=255)                                                                                                                         acCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGTCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa           <  1:692663/151‑1 (MQ=255)                                                                                                                           cTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt         >  1:693608/1‑151 (MQ=255)                                                                                                                            tttAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGATACTTAACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACTGTTCATCAAAt          >  2:782065/1‑149 (MQ=255)                                                                                                                             ttAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc        <  2:801737/150‑1 (MQ=255)                                                                                                                              tAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct       <  2:888400/150‑1 (MQ=255)                                                                                                                                aTGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTcc     >  1:572008/1‑150 (MQ=255)                                                                                                                                aTGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTcc     >  1:667901/1‑150 (MQ=255)                                                                                                                                aTGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCa    <  1:252014/151‑1 (MQ=255)                                                                                                                                  gACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc  >  1:545891/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                        GCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGC  >  NC_000913/2837839‑2838118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑