Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,791	T→C	Y153H (TAT→CAT)	ygbK →	FliA‑regulated DUF1537 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,791	0	T	C	100.0%	246.2 / NA	66	Y153H (TAT→CAT)	ygbK	FliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/27);  total (39/27)

TGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTG  >  NC_000913/2862646‑2862937                                                                                                                                                  |                                                                                                                                                   tGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCtt                                                                                                                                               >  2:731535/1‑151 (MQ=255)    tctcGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTccc                                                                                                                                            >  1:819330/1‑151 (MQ=255)     ctcGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTcccc                                                                                                                                           >  2:424507/1‑151 (MQ=255)      tcGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCCACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGGCAGCCATCTTcccc                                                                                                                                           >  2:165843/1‑150 (MQ=255)         acacGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCt                                                                                                                                       <  2:45630/151‑1 (MQ=255)                gTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCTGGGGTCTTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGtt                                                                                                                                   >  2:430909/1‑148 (MQ=255)                    aCGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAgcg                                                                                                                             <  1:1828/150‑1 (MQ=255)                    aCGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAgcg                                                                                                                             <  1:902088/150‑1 (MQ=255)                       gTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAAt                                                                                                                         >  2:686264/1‑151 (MQ=255)                         cttctCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATcc                                                                                                                       >  1:782571/1‑151 (MQ=255)                              ctcCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAgg                                                                                                                  >  2:627642/1‑151 (MQ=255)                                ccGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAgggt                                                                                                                >  2:17112/1‑150 (MQ=255)                                ccGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAg                                                                                                                   <  2:82563/148‑1 (MQ=255)                                     ccTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGtgctgc                                                                                                           >  1:271467/1‑151 (MQ=255)                                        gCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTgcgg                                                                                                         <  1:640832/150‑1 (MQ=255)                                        gCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTgcgg                                                                                                         <  1:258484/150‑1 (MQ=255)                                         ccGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTgcggcg                                                                                                       <  2:639957/151‑1 (MQ=255)                                          cGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGcgt                                                                                                      >  2:759815/1‑151 (MQ=255)                                          cGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGcgt                                                                                                      <  2:9830/151‑1 (MQ=255)                                              cAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGtt                                                                                                  >  1:333055/1‑151 (MQ=255)                                              cAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGtt                                                                                                  >  1:888171/1‑151 (MQ=255)                                                 cGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCg                                                                                               >  1:148572/1‑151 (MQ=255)                                                  ggACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCg                                                                                               <  2:690759/150‑1 (MQ=255)                                                  ggACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCg                                                                                               >  1:690808/1‑150 (MQ=255)                                                   gACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGc                                                                                             >  1:411363/1‑151 (MQ=255)                                                        aCGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATg                                                                                        <  1:331890/151‑1 (MQ=255)                                                        aCGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATg                                                                                        >  1:555397/1‑151 (MQ=255)                                                         cGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGt                                                                                       <  2:475074/151‑1 (MQ=255)                                                          ggTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGtt                                                                                      <  2:897134/151‑1 (MQ=255)                                                               aTCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCg                                                                                  <  1:753181/150‑1 (MQ=255)                                                               aTCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCg                                                                                  >  2:59635/1‑150 (MQ=255)                                                                 cAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCACATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAAc                                                                               >  2:743830/1‑151 (MQ=255)                                                                  aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACa                                                                              <  2:554389/151‑1 (MQ=255)                                                                      gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATTACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAgg                                                                           >  2:145385/1‑150 (MQ=255)                                                                      gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgt                                                                          >  2:6885/1‑151 (MQ=255)                                                                       tATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtg                                                                         >  1:808179/1‑151 (MQ=255)                                                                       tATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtg                                                                         >  1:39/1‑151 (MQ=255)                                                                          ttGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTgg                                                                      >  1:732355/1‑151 (MQ=255)                                                                               cGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGcc                                                                 >  2:391944/1‑151 (MQ=255)                                                                                       aTCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCGTCTTCCCCGTCGGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                                                          <  2:711050/150‑1 (MQ=255)                                                                                       aTCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                                                          >  2:39943/1‑150 (MQ=255)                                                                                          aaCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCa                                                         >  1:405228/1‑148 (MQ=255)                                                                                           aCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAagag                                                     <  1:131157/151‑1 (MQ=255)                                                                                              gctgGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTg                                                  <  2:288023/151‑1 (MQ=255)                                                                                              gctgGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTg                                                  <  1:636093/151‑1 (MQ=255)                                                                                                    cGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGc                                            >  2:412375/1‑151 (MQ=255)                                                                                                     gAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCt                                           >  1:887339/1‑151 (MQ=255)                                                                                                     gAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCt                                           >  2:183598/1‑151 (MQ=255)                                                                                                       aTCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCtt                                          >  1:486916/1‑150 (MQ=255)                                                                                                       aTCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTa                                         <  2:553983/151‑1 (MQ=255)                                                                                                              aTGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCa                                    >  2:563070/1‑149 (MQ=255)                                                                                                                gcgcCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAagag                                <  1:649997/151‑1 (MQ=255)                                                                                                                 cgcCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAgg                               >  2:412175/1‑151 (MQ=255)                                                                                                                  gcCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAggg                              <  1:865038/151‑1 (MQ=255)                                                                                                                       cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                         >  1:871918/1‑151 (MQ=255)                                                                                                                       cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                         >  1:203008/1‑151 (MQ=255)                                                                                                                       cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                         >  1:197679/1‑151 (MQ=255)                                                                                                                          ccGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCt                      <  1:731584/151‑1 (MQ=255)                                                                                                                                        gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGa         <  1:424552/150‑1 (MQ=255)                                                                                                                                        gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGAt        >  2:544266/1‑151 (MQ=255)                                                                                                                                         aCCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGa         <  2:900505/149‑1 (MQ=255)                                                                                                                                         aCCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGa         >  1:900578/1‑149 (MQ=255)                                                                                                                                             aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt   <  2:372960/151‑1 (MQ=255)                                                                                                                                             aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt   <  2:311322/151‑1 (MQ=255)                                                                                                                                             aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt   <  1:777061/151‑1 (MQ=255)                                                                                                                                               aGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTg  >  1:798088/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTG  >  NC_000913/2862646‑2862937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑