Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,827	C→T	R165C (CGC→TGC)	ygbK →	FliA‑regulated DUF1537 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,827	0	C	T	100.0%	219.0 / NA	59	R165C (CGC→TGC)	ygbK	FliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/28);  total (31/28)

CCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTT  >  NC_000913/2862682‑2862972                                                                                                                                                  |                                                                                                                                                  ccTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGtgctgc                                                                                                                                              >  1:271467/1‑151 (MQ=255)    gCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTgcgg                                                                                                                                            <  1:258484/150‑1 (MQ=255)    gCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTgcgg                                                                                                                                            <  1:640832/150‑1 (MQ=255)     ccGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTgcggcg                                                                                                                                          <  2:639957/151‑1 (MQ=255)      cGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGcgt                                                                                                                                         <  2:9830/151‑1 (MQ=255)      cGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGcgt                                                                                                                                         >  2:759815/1‑151 (MQ=255)          cAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGtt                                                                                                                                     >  1:888171/1‑151 (MQ=255)          cAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGtt                                                                                                                                     >  1:333055/1‑151 (MQ=255)             cGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCg                                                                                                                                  >  1:148572/1‑151 (MQ=255)              ggACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCg                                                                                                                                  >  1:690808/1‑150 (MQ=255)              ggACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCg                                                                                                                                  <  2:690759/150‑1 (MQ=255)               gACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGc                                                                                                                                >  1:411363/1‑151 (MQ=255)                    aCGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATg                                                                                                                           >  1:555397/1‑151 (MQ=255)                    aCGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATg                                                                                                                           <  1:331890/151‑1 (MQ=255)                     cGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGt                                                                                                                          <  2:475074/151‑1 (MQ=255)                      ggTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGtt                                                                                                                         <  2:897134/151‑1 (MQ=255)                           aTCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCg                                                                                                                     <  1:753181/150‑1 (MQ=255)                           aTCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCg                                                                                                                     >  2:59635/1‑150 (MQ=255)                             cAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCACATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAAc                                                                                                                  >  2:743830/1‑151 (MQ=255)                              aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACa                                                                                                                 <  2:554389/151‑1 (MQ=255)                                  gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATTACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAgg                                                                                                              >  2:145385/1‑150 (MQ=255)                                  gTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgt                                                                                                             >  2:6885/1‑151 (MQ=255)                                   tATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtg                                                                                                            >  1:39/1‑151 (MQ=255)                                   tATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtg                                                                                                            >  1:808179/1‑151 (MQ=255)                                      ttGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTgg                                                                                                         >  1:732355/1‑151 (MQ=255)                                           cGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGcc                                                                                                    >  2:391944/1‑151 (MQ=255)                                                   aTCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCGTCTTCCCCGTCGGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                                                                                             <  2:711050/150‑1 (MQ=255)                                                   aTCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTc                                                                                             >  2:39943/1‑150 (MQ=255)                                                      aaCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCa                                                                                            >  1:405228/1‑148 (MQ=255)                                                       aCTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAagag                                                                                        <  1:131157/151‑1 (MQ=255)                                                          gctgGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTg                                                                                     <  2:288023/151‑1 (MQ=255)                                                          gctgGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTg                                                                                     <  1:636093/151‑1 (MQ=255)                                                                cGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGc                                                                               >  2:412375/1‑151 (MQ=255)                                                                 gAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCt                                                                              >  1:887339/1‑151 (MQ=255)                                                                 gAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCt                                                                              >  2:183598/1‑151 (MQ=255)                                                                   aTCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCtt                                                                             >  1:486916/1‑150 (MQ=255)                                                                   aTCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTa                                                                            <  2:553983/151‑1 (MQ=255)                                                                          aTGCGCCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCa                                                                       >  2:563070/1‑149 (MQ=255)                                                                            gcgcCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAagag                                                                   <  1:649997/151‑1 (MQ=255)                                                                             cgcCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAgg                                                                  >  2:412175/1‑151 (MQ=255)                                                                              gcCATCACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAggg                                                                 <  1:865038/151‑1 (MQ=255)                                                                                   cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                                                            >  1:871918/1‑151 (MQ=255)                                                                                   cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                                                            >  1:203008/1‑151 (MQ=255)                                                                                   cACCCGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTAcc                                                            >  1:197679/1‑151 (MQ=255)                                                                                      ccGGTAAATCCCATGACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCt                                                         <  1:731584/151‑1 (MQ=255)                                                                                                    gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGa                                            <  1:424552/150‑1 (MQ=255)                                                                                                    gACCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGAt                                           >  2:544266/1‑151 (MQ=255)                                                                                                     aCCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGa                                            <  2:900505/149‑1 (MQ=255)                                                                                                     aCCGACAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGa                                            >  1:900578/1‑149 (MQ=255)                                                                                                         aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt                                      <  1:777061/151‑1 (MQ=255)                                                                                                         aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt                                      <  2:372960/151‑1 (MQ=255)                                                                                                         aCAGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCt                                      <  2:311322/151‑1 (MQ=255)                                                                                                           aGCCATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTg                                     >  1:798088/1‑150 (MQ=255)                                                                                                                   tCCCCGTCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGa                                <  2:337091/147‑1 (MQ=255)                                                                                                                         tCTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTg                      >  1:899588/1‑151 (MQ=255)                                                                                                                          cTGGTTGAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTgg                     >  2:773405/1‑151 (MQ=255)                                                                                                                                gAAGCGCAATCCACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAAtt                <  2:411010/150‑1 (MQ=255)                                                                                                                                       aaTCCGCAGGGTGGTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGgaga        <  1:564158/151‑1 (MQ=255)                                                                                                                                          ccACAGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCAGCTGGAAATTCAGGGAGAAGc     <  2:12172/151‑1 (MQ=255)                                                                                                                                             caGGGTGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCtt  <  1:516664/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTT  >  NC_000913/2862682‑2862972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑