Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,937,090	T→C	W503R (TGG→CGG)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,937,090	0	T	C	100.0%	182.2 / NA	52	W503R (TGG→CGG)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/14);  total (38/14)

GTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCTGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTAT  >  NC_000913/2936949‑2937237                                                                                                                                              |                                                                                                                                                    gTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCAc                                                                                                                                            <  1:452127/151‑1 (MQ=255)   cccGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACcg                                                                                                                                          >  1:804516/1‑151 (MQ=255)      gTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCgcgc                                                                                                                                       >  2:445097/1‑151 (MQ=255)       ttCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGcc                                                                                                                                      >  1:350418/1‑151 (MQ=255)       ttCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGcc                                                                                                                                      >  2:665957/1‑151 (MQ=255)         cACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCt                                                                                                                                     <  1:11086/150‑1 (MQ=255)          aCCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTc                                                                                                                                    >  1:371957/1‑150 (MQ=255)             aTGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCg                                                                                                                                >  2:323894/1‑151 (MQ=255)               gACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGt                                                                                                                              >  1:711133/1‑151 (MQ=255)                    gtgtCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAgg                                                                                                                         <  1:89270/151‑1 (MQ=255)                    gtgtCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAgg                                                                                                                         >  2:216489/1‑151 (MQ=255)                        cAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCg                                                                                                                     >  2:456676/1‑151 (MQ=255)                                 caAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGAt                                                                                                            >  1:451173/1‑151 (MQ=255)                                  aaaGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGAtg                                                                                                           >  2:693230/1‑151 (MQ=255)                                   aaGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGAtgt                                                                                                          >  2:436035/1‑151 (MQ=255)                                     ggCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATgtgt                                                                                                        <  2:285991/151‑1 (MQ=255)                                       ccTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTAc                                                                                                      >  2:565327/1‑151 (MQ=255)                                       ccTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTAc                                                                                                      <  1:317376/151‑1 (MQ=255)                                         tGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTc                                                                                                    >  1:657869/1‑151 (MQ=255)                                           ggACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCgg                                                                                                  >  2:111886/1‑151 (MQ=255)                                                ggTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATg                                                                                             <  1:603234/151‑1 (MQ=255)                                                  tACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGc                                                                                           >  2:845046/1‑151 (MQ=255)                                                      gCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAAc                                                                                       >  1:909803/1‑151 (MQ=255)                                                            cgcgGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGgc                                                                                 <  1:155606/151‑1 (MQ=255)                                                             gcgGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGgcg                                                                                <  2:162208/151‑1 (MQ=255)                                                                gAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTa                                                                             <  1:113233/151‑1 (MQ=255)                                                                             tGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCt                                                                >  2:181219/1‑151 (MQ=255)                                                                             tGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCt                                                                >  1:634846/1‑151 (MQ=255)                                                                                 aTTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGAcc                                                            <  1:686892/151‑1 (MQ=255)                                                                                      cGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCgg                                                       >  1:380242/1‑151 (MQ=255)                                                                                       gAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGc                                                      >  2:172648/1‑151 (MQ=255)                                                                                           gATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACg                                                  >  2:800206/1‑151 (MQ=255)                                                                                            aTGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGt                                                 >  1:674547/1‑151 (MQ=255)                                                                                                gCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGgc                                             >  1:337999/1‑151 (MQ=255)                                                                                                   tGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGgcgc                                           >  2:187836/1‑150 (MQ=255)                                                                                                   tGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGgcgc                                           >  1:258077/1‑150 (MQ=255)                                                                                                     aCATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGgcgc                                           >  2:34237/1‑148 (MQ=255)                                                                                                      cATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGAc                                       >  1:380052/1‑151 (MQ=255)                                                                                                      cATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGAGCTAACCATGGGGTTCTTACCATCGGCCACGTTGGCGCAGa                                        <  2:651405/150‑1 (MQ=255)                                                                                                       aTCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACt                                      >  1:900192/1‑151 (MQ=255)                                                                                                         ccTCAACAAACGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACttt                                    >  1:579599/1‑151 (MQ=255)                                                                                                                aaaCGCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCActc                             >  2:568954/1‑151 (MQ=255)                                                                                                                    gCACCAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGc                         >  2:177366/1‑151 (MQ=255)                                                                                                                      aCCAACTCACCCTGGCCAACCGCCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACGCGGTAATGGCGATCTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCt                       <  2:846946/151‑1 (MQ=255)                                                                                                                        cAACTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTcc                     >  2:794229/1‑151 (MQ=255)                                                                                                                          aCTCAACCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCAt                   >  2:722576/1‑151 (MQ=255)                                                                                                                              aaCCTTGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGACGAACTGGGGCGCTAATCATGGCGTTCTGACCATCGGCGACGTTGGCGCAGACTTTATCACTCTCGCCTCCAtgc                 >  2:452809/1‑149 (MQ=255)                                                                                                                              aaCCTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCAtgctg               >  1:153658/1‑151 (MQ=255)                                                                                                                                ccTGGCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCg             >  1:329857/1‑151 (MQ=255)                                                                                                                                   ggCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGt            <  2:337973/149‑1 (MQ=255)                                                                                                                                   ggCCAACCACCCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTa           <  1:602974/150‑1 (MQ=255)                                                                                                                                           accCGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGgtat  >  2:644600/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    GTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCTGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTAT  >  NC_000913/2936949‑2937237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑