Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,722	T→C	T135A (ACG→GCG)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,722	0	T	C	100.0%	183.5 / NA	53	T135A (ACG→GCG)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/34);  total (19/34)

CTCCGTCAGCCCCATACTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCG  >  NC_000913/2983576‑2983867                                                                                                                                                   |                                                                                                                                                  cTCCGTCAGCCCCATACTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATgc                                                                                                                                               >  1:654932/1‑151 (MQ=255)   ccGTCAGCCCCATACTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATgcg                                                                                                                                              <  1:859658/150‑1 (MQ=255)           cccATACTCAATTGGCAGGTTTCCAGTGCAGCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTAAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTgc                                                                                                                                       <  2:181243/149‑1 (MQ=255)           cccATACTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTgcgc                                                                                                                                     >  1:764977/1‑151 (MQ=255)            ccATACTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTgcgc                                                                                                                                     >  1:496948/1‑150 (MQ=255)                aCTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAAtt                                                                                                                                 <  2:264368/150‑1 (MQ=255)                aCTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGAGACTGGCGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAAtt                                                                                                                                 <  2:161916/150‑1 (MQ=255)                 cTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGt                                                                                                                               >  1:89352/1‑151 (MQ=255)                  tCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGt                                                                                                                               >  2:117627/1‑150 (MQ=255)                   cAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGta                                                                                                                              >  1:695052/1‑150 (MQ=255)                   cAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGt                                                                                                                               <  1:243496/149‑1 (MQ=255)                     aTTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGAAGATACTTCGTCCGGTTTGACTTTTTTGGTGGGATACGTCGCATGCGCGCGTGCGCAATTGtagt                                                                                                                            <  2:207738/150‑3 (MQ=255)                        ggCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATaaa                                                                                                                         >  1:690738/1‑150 (MQ=255)                         gCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAAc                                                                                                                       >  2:361463/1‑151 (MQ=255)                                  cAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGa                                                                                                              <  2:384564/151‑1 (MQ=255)                                      aCATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTg                                                                                                           <  1:144563/150‑1 (MQ=255)                                      aCATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTg                                                                                                           <  1:585999/150‑1 (MQ=255)                                            gggACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTAt                                                                                                    <  1:651697/151‑1 (MQ=255)                                                  aaaTATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATAc                                                                                              >  1:100913/1‑151 (MQ=255)                                                     tatTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTgg                                                                                           >  2:773881/1‑151 (MQ=255)                                                         tGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCaa                                                                                        >  1:171811/1‑150 (MQ=255)                                                          gTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCaa                                                                                        >  1:51181/1‑149 (MQ=255)                                                          gTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAg                                                                                      <  1:448862/151‑1 (MQ=255)                                                            tAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGAc                                                                                    >  2:756628/1‑151 (MQ=255)                                                              aTCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACa                                                                                   >  2:215030/1‑150 (MQ=255)                                                                cGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAAct                                                                                >  1:513194/1‑151 (MQ=255)                                                                 gTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAActt                                                                               <  1:882666/151‑1 (MQ=255)                                                                   gCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAActtc                                                                              <  1:506811/150‑1 (MQ=255)                                                                      aCGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCtttt                                                                          <  2:631060/151‑1 (MQ=255)                                                                       cGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTg                                                                         <  1:565451/151‑1 (MQ=255)                                                                        ggTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGc                                                                        <  2:246302/151‑1 (MQ=255)                                                                               ggTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTa                                                                 <  1:324844/151‑1 (MQ=255)                                                                                gTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTAc                                                                <  2:900012/151‑1 (MQ=255)                                                                                  gAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTAcc                                                               <  1:649345/150‑1 (MQ=255)                                                                                   aGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCaa                                                             <  1:823559/151‑1 (MQ=255)                                                                                        aTCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATa                                                         <  2:496899/150‑1 (MQ=255)                                                                                          cGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACa                                                       <  1:23604/150‑1 (MQ=255)                                                                                          cGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAg                                                      <  1:1354/151‑1 (MQ=255)                                                                                          cGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGATGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACa                                                       <  2:323671/150‑1 (MQ=255)                                                                                               aaCGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGc                                                   <  1:58867/149‑1 (MQ=255)                                                                                               aaCGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGt                                                 >  2:223250/1‑151 (MQ=255)                                                                                                 cGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTcg                                               >  1:775132/1‑151 (MQ=255)                                                                                                          agaTACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCg                                      >  1:259920/1‑151 (MQ=255)                                                                                                                 tCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTc                                 <  1:579362/149‑1 (MQ=255)                                                                                                                  cGTCCGATGTGTCTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACACCTTCTTTTGCTCCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCAtg                              <  2:405588/151‑2 (MQ=255)                                                                                                                       ggTGTGACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCAtt                         <  1:94798/151‑1 (MQ=255)                                                                                                                           tgACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGcctggg                       <  2:94496/149‑6 (MQ=255)                                                                                                                            gACTTTTTTGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGcc                     <  2:168464/150‑1 (MQ=255)                                                                                                                                tttttGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCg                 <  2:576286/150‑1 (MQ=255)                                                                                                                                tttttGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCg                 <  2:439631/150‑1 (MQ=255)                                                                                                                                  tttGGTGGGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGAc               <  2:167413/150‑1 (MQ=255)                                                                                                                                     ggtggGATACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGt            <  1:365745/150‑1 (MQ=255)                                                                                                                                            tACGTCGCATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTAc    >  1:102350/1‑151 (MQ=255)                                                                                                                                              cgtcgcATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCg  >  2:66952/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CTCCGTCAGCCCCATACTCAATTGGCAGGTTTCCAGTACATCCGGGACAAAATATTTGTTAATCGTGCGACGGTTACTGGTGAGGTTATCGCCTAACGTGACTGGAGATACTTCGTCCGGTGTGACTTTTTTGGTGGGATACGTCGTATGCGCGGGTGCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCG  >  NC_000913/2983576‑2983867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑