Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,986,699	T→C	L285L (TTG→CTG)	yqeG →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,986,699	0	T	C	100.0%	185.4 / NA	50	L285L (TTG→CTG)	yqeG	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/27);  total (23/27)

GCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCATTTAACCGC  >  NC_000913/2986555‑2986845                                                                                                                                                 |                                                                                                                                                   gCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCa                                                                                                                                               >  2:334669/1‑150 (MQ=255)   ttATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCCTGGCTGTCAAtt                                                                                                                                            >  1:664900/1‑151 (MQ=255)     aTCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTc                                                                                                                                          >  1:804901/1‑151 (MQ=255)     aTCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTc                                                                                                                                          >  1:618902/1‑151 (MQ=255)         catcatCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTc                                                                                                                                          <  2:365122/147‑1 (MQ=255)         catcatCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTc                                                                                                                                          <  1:734888/147‑1 (MQ=255)         catcatCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCg                                                                                                                                        <  1:635882/149‑1 (MQ=255)          atcatcTGCATAAGTGTACTGTTCCTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCggg                                                                                                                                      >  1:205043/1‑150 (MQ=255)            catcTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGatt                                                                                                                                   >  1:785050/1‑151 (MQ=255)               cTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGattatt                                                                                                                                >  2:215175/1‑151 (MQ=255)                 gCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTg                                                                                                                               <  1:901215/150‑1 (MQ=255)                         gtACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCagtag                                                                                                                      <  1:909513/151‑1 (MQ=255)                              gTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCg                                                                                                                 <  2:726678/151‑1 (MQ=255)                                   ttGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGt                                                                                                             <  1:12842/150‑1 (MQ=255)                                   ttGTCTTTAGCTGCCTGCTTTCTATTCCACCTGCGTATATTGAAGCTGCTAAAGAAAAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGAt                                                                                                               <  2:243897/148‑1 (MQ=255)                                    tGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTc                                                                                                            <  2:248842/150‑1 (MQ=255)                                    tGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCg                                                                                                           >  1:4081/1‑151 (MQ=255)                                        tttAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAAt                                                                                                       >  1:231166/1‑151 (MQ=255)                                        tttAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAAt                                                                                                       >  1:358361/1‑151 (MQ=255)                                         ttAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAAt                                                                                                       <  1:686311/150‑1 (MQ=255)                                          tAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATc                                                                                                      <  1:134532/150‑1 (MQ=255)                                             ctgcctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCGGTAGTTGCGATGTCGAAATCATTc                                                                                                  >  2:774524/1‑151 (MQ=255)                                               gcctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTc                                                                                                  <  1:192812/149‑1 (MQ=255)                                               gcctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCt                                                                                                >  1:563144/1‑151 (MQ=255)                                               gcctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCt                                                                                                >  2:619572/1‑151 (MQ=255)                                                cctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTg                                                                                               <  1:744553/151‑1 (MQ=255)                                                      ttCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGgtac                                                                                          <  1:826187/150‑1 (MQ=255)                                                          aTTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGgtacgt                                                                                        <  2:570991/148‑1 (MQ=255)                                                             ccACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTg                                                                                  <  1:272226/151‑1 (MQ=255)                                                                        tatTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGa                                                                        >  2:376216/1‑150 (MQ=255)                                                                             aaGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGc                                                                  >  1:423616/1‑151 (MQ=255)                                                                              aGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGcc                                                                 >  2:673121/1‑151 (MQ=255)                                                                               gctgctAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCca                                                                <  1:109421/151‑1 (MQ=255)                                                                                 tgctAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCcac                                                               >  2:267412/1‑150 (MQ=255)                                                                                    tAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACag                                                             >  1:49929/1‑149 (MQ=255)                                                                                    tAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACag                                                             >  2:89125/1‑149 (MQ=255)                                                                                        gaagaagGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGgtcg                                                       <  1:497169/151‑1 (MQ=255)                                                                                                       aTTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTAc                                        <  1:144478/151‑1 (MQ=255)                                                                                                            aTCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTAcagc                                     >  2:628075/1‑149 (MQ=255)                                                                                                              cGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAgg                                  <  1:182853/150‑1 (MQ=255)                                                                                                              cGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAgg                                  <  2:789315/150‑1 (MQ=255)                                                                                                               ggCGCTTTCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGtt                                <  1:440511/151‑1 (MQ=255)                                                                                                                     ttCTATGCTGCCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGgtgt                           >  2:225995/1‑150 (MQ=255)                                                                                                                           gctgcCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGaa                     <  1:166387/150‑1 (MQ=255)                                                                                                                           gctgcCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGaa                     <  2:641341/150‑1 (MQ=255)                                                                                                                              gcCGAACGCCCCAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGaaaa                   <  2:312269/149‑1 (MQ=255)                                                                                                                                      ccccAGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCAtt         <  1:689400/151‑1 (MQ=255)                                                                                                                                          aGCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCATTTAAc     <  2:708423/151‑1 (MQ=255)                                                                                                                                           gCATGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCATTTAAcc    >  1:186443/1‑151 (MQ=255)                                                                                                                                             aTGGCTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCATTTAACcgc  >  1:92148/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCATTCCTGGGTACGTACTTTGGCGTTATTGAAGGTGCCACAGAGGTCGTCAAAACAACACTACAGCAGGTTGGTGTAAAGAAAAGTCGTGCATTTAACCGC  >  NC_000913/2986555‑2986845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑