Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,391	T→C	*311Q (TAA→CAA)	yqeA →	putative amino acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,391	0	T	C	100.0%	191.7 / NA	50	*311Q (TAA→CAA)	yqeA	putative amino acid kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

GGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATA  >  NC_000913/3012244‑3012537                                                                                                                                                    |                                                                                                                                                   ggTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAAt                                                                                                                                                 >  2:767990/1‑151 (MQ=255)    cATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAAttt                                                                                                                                               >  1:453214/1‑150 (MQ=255)     aTTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTaa                                                                                                                                             <  1:207363/151‑1 (MQ=255)              aaGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCgggt                                                                                                                                    >  1:57311/1‑151 (MQ=255)               aGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCgggtg                                                                                                                                   <  1:151017/151‑1 (MQ=255)                      aTGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAAt                                                                                                                             >  2:637207/1‑150 (MQ=255)                       tgctgcCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATAc                                                                                                                           >  1:602812/1‑151 (MQ=255)                         ctgcCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATAc                                                                                                                           >  2:24393/1‑149 (MQ=255)                            ccTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATcc                                                                                                                      >  2:330935/1‑151 (MQ=255)                               aaaGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCgg                                                                                                                    <  1:879077/150‑1 (MQ=255)                               aaaGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCgg                                                                                                                    <  1:758484/150‑1 (MQ=255)                               aaaGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGc                                                                                                                   >  2:876015/1‑151 (MQ=255)                                aaGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGcc                                                                                                                  <  1:133923/151‑1 (MQ=255)                                   tGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCaa                                                                                                                >  2:160610/1‑150 (MQ=255)                                              gccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaagt                                                                                                    <  2:123441/151‑1 (MQ=255)                                              gccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaagt                                                                                                    <  1:276434/151‑1 (MQ=255)                                                cTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaagta                                                                                                   >  2:426522/1‑150 (MQ=255)                                                        ctcgctcTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCtt                                                                                          >  2:418256/1‑151 (MQ=255)                                                          cgctcTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCttt                                                                                         <  1:185839/150‑1 (MQ=255)                                                               tcGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGAt                                                                                   >  2:434718/1‑151 (MQ=255)                                                                      ggTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGAt                                                                            >  1:336073/1‑151 (MQ=255)                                                                       gTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATg                                                                           <  2:176232/151‑1 (MQ=255)                                                                            gAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATgcggc                                                                       <  1:877296/150‑1 (MQ=255)                                                                                         ccGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATc                                                         <  2:883337/151‑1 (MQ=255)                                                                                          cGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATcc                                                        <  2:312345/151‑1 (MQ=255)                                                                                            tATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGt                                                      >  1:418934/1‑151 (MQ=255)                                                                                               tGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTcc                                                    <  1:572018/150‑1 (MQ=255)                                                                                                gAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCt                                                   >  2:853635/1‑150 (MQ=255)                                                                                                 aGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTAc                                                 >  2:781059/1‑151 (MQ=255)                                                                                                       gCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAAc                                            >  1:368458/1‑150 (MQ=255)                                                                                                         gAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATc                                         >  1:31056/1‑151 (MQ=255)                                                                                                            agaagGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGc                                       >  1:484246/1‑150 (MQ=255)                                                                                                            agaagGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCa                                      <  2:528351/151‑1 (MQ=255)                                                                                                                    ttGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATCCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCaa                               >  1:716168/1‑150 (MQ=255)                                                                                                                      gAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAg                            <  1:262199/151‑1 (MQ=255)                                                                                                                       aaGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGa                           <  2:796294/151‑1 (MQ=255)                                                                                                                        aGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGAt                          <  2:223539/151‑1 (MQ=255)                                                                                                                         ggCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGAtt                         <  1:496559/151‑1 (MQ=255)                                                                                                                           cAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGTTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGc                       <  2:76169/151‑1 (MQ=255)                                                                                                                           cAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGc                       <  2:401745/151‑1 (MQ=255)                                                                                                                           cAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGc                       >  2:884569/1‑151 (MQ=255)                                                                                                                            aaaaCAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCa                      <  1:417987/151‑1 (MQ=255)                                                                                                                               aCAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATg                   <  1:144521/151‑1 (MQ=255)                                                                                                                                 aGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCa                 <  1:436686/151‑1 (MQ=255)                                                                                                                                      cGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGt            >  1:188870/1‑151 (MQ=255)                                                                                                                                          gATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAgg         <  1:488404/150‑1 (MQ=255)                                                                                                                                          gATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGc        <  2:909373/151‑1 (MQ=255)                                                                                                                                            tttGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCt      >  1:837450/1‑151 (MQ=255)                                                                                                                                              tGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTg     >  2:370059/1‑150 (MQ=255)                                                                                                                                                 cAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATa  <  1:518106/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATA  >  NC_000913/3012244‑3012537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑